Reporter Assay Kits

Reporter assay kits are available from a number of suppliers to detect and measure levels of reporter genes, which serve as markers for various elements of gene expression. Reporters are genetically constructed along with a regulatory sequence of a gene of interest and introduced into a host cell. A common reporter, luciferase, reacts with the substrate luciferin to produce luminescence, which can be quantified by a luminometer. Fluorescent proteins, such as GFP and RFP, produce fluorescence upon expression. Other reporter systems utilize beta-galactosidase, beta-glucuronidase (GUS), and secreted embryonic alkaline phosphatase (SEAP). Visit the supplier page for more information, including assay principle and components.