Luciferase Assay System, 10-Pack E1501 from Promega

Product Specs
ItemLuciferase Assay System, 10-Pack
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE1501
Quantity1,000 assays
ReactivityMammalian, Bacteria, Plant
SensitivityTo 10–20 moles of luciferase.
MethodLuciferase
Sample TypeMammalian cell. Bacteria&,Plant cells
Detection TargetLuciferase
SpeciesMammalian. Bacteria,Plant
Assay Time5 minutes

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Lipid Nanoparticle Surface Engineering with Heparosan Polysaccharides for Safe and Effective mRNA Delivery In Vitro and In VivoACS Appl Mater InterfacesMay 27, 2026Yuxin He, Lin Wang, Rameswari Velayutham, Trisha I. Valerio, Samantha D. Ricketts, Yuanhong Sun, Anand C. Annan, James Bowman, Thao Tran, Mobina Mohammadnejad, et al.(5-moUTP) (APExBIO, R1016, Houston, TX, United States); Luciferase Assay System (Promega Products, E1501, Madison, WI, United States); d-luciferin potassium salt [Gold Biotechnology (U.S. Registration No 3,257,927)]. Cell Culture

(2) WAT-to-BAT communication facilitates the sustained activation of BAT thermogenesis during cold exposureCell DiscovMay 26, 2026Jieyuan Xue, Ding Chen, Chenfeng Wang, Jue Wang, Jiayu Zhou, Wenyan Li, Junxi Qian, Yuanji Huang, Liang Fang, Hongyong Song, et al.cells were washed with PBS. Luciferase activity was measured using a Luciferase Assay System (Promega, E1501) following the manufacturer’s instructions. Establishment of stable overexpression and knockdown cell

(3) Redirection of SARS-CoV-2 to phagocytes by intranasal sACE2-Fc as a universal decoy confers complete prophylactic protectionElifeMay 18, 2026Jingyi Wang, Jiangchuan Li, Alex WH Chin, Bin Luo, Junkang Wei, Jiale Qiu, Jianwei Ren, Yin Xia, Thomas Braun, Leo LM Poon, et al.cells in the presence of 8 µg/ml polybrene. Infectivity was assessed via a luciferase assay (Promega #E1501). Protein production and purification 293T and Expi293F cells were transfected with HDM-CMV-sACE2(-Fc)(-LALA)-his

(4) Chimeric autoantibody receptor T cells for targeted depletion of myeloperoxidase-specific anti-neutrophil cytoplasmic antibody-producing B cellsFront ImmunolMay 8, 2026Heather M. Sosnoski, William Aguilar, Shawna K. Brookens, David A. Degaramo, John T. Keane, Hyeon-Gyu S. Lewis, Damodar Gullipalli, Wenchao Song, Avery D. Poseyintracellular CBG luciferase. First, cells were washed and lysed with Reporter Lysis 5× Buffer (Promega, #E1501) and frozen at −80°C. Plates were thawed and 40 μl of supernatant combined with 50 μl of luciferase

(5) Allogeneic CRISPR-Engineered CAR-T Cells Drive Potent Antitumor Activity in Solid TumorsbioRxivMay 5, 2026Mingyu Huo, Dan Li, Nan Li, Alex Quan, Tianyuzhou Liang, Dan Henderson, Jason Sagert, Minh Pham, Luke Hanley, Kelly Maeng, et al.lysed by the passive lysis buffer (E1941, Promega) at room temperature. Add luciferase assay reagent (E1501, Promega) to each sample and then measure the light produced using the SpectraMax (Molecular Devices)

(6) Discovery and Preclinical Validation of a Clinically Optimized Mitochondrial Complex I Modulator for Alzheimer’s DiseasebioRxivApril 17, 2026Sergey Trushin, Thi Kim Oanh Nguyen, Mark Ostroot, Alexander Galkin, Toshihiko Nambara, Wenyan Lu, Takahisa Kanekiyo, Graham Johnson, Eugenia Trushinang/ml) for 6 h. NF-κB luciferase activity was measured using the Luciferase Assay System (Promega, E1501). n = 4 independent experiments. e C273 restores mitochondrial biogenesis, expression of ETC complexes,

(7) Targeting Melanoma-Associated Fibroblasts to Overcome Cancer Stem Cell-Driven Drug ResistanceJournal of the American College of SurgeonsApril 1, 2026Hongwei Shao, Mecker Moller, Nga Le, Yulexi Y Ortiz, Omaida C Velazquez, Zhao-Jun Liubovine serum. Cells were stably transduced with DsRed (Clontech, 632496) or firefly luciferase (Promega, E1501). Cells were maintained in DMEM with 10% fetal bovine serum and 1% penicillin-streptomycin. Three-dimensional

(8) ILC3s mediate intestinal immune-epithelial interactions via TGF-β1 activationMucosal ImmunologyApril 1, 2026Diana Coman, John W. Bassett, Isabelle Coales, Ainize Peña-Cearra, Emily Read, Emma H. Kromann, Daniel Brice, Zuzanna Łukasik, Helena Paidassi, Matthew R. Hepworth, et al.DMEM medium (Table 2). After 20 hours of incubation at 37 °C and 5 % CO2, a luciferase assay (Promega E1501) was performed and luminescence measured, for further details, see online supplemental materials and

(9) MMP14 is a safe target of CAR-T therapy against liver cancer and metastasisJ Transl MedMarch 26, 2026Ye Yu, Shirui Zou, Jiawei Fan, Dong Yang, Nan Liu, Zhiyong Bai, Yongjie Zhu, Lihong Li, Dan Cao, Xudong Zhao, et al.mixtures were incubated for 24 h. Luciferase activity was measured via a Luciferase Assay System (Promega, E1501). The release of TNF-α and IFN-γ in culture medium was measured via a specific ELISA kit in accordance

(10) Computational Design of Broad-Spectrum Ebola Antibodies through Framework and Complementarity-Determining Region Synergistic OptimizationResearch (Wash D C)March 23, 2026Xinhui Zhang, Xiuying Liu, Jingya Zhou, Peixiang Gao, Shengnan Pan, Xuehua Yang, Huarui Duan, Yi Liao, Fangyuan Zhang, Xuemeng Dong, et al.activity was measured by incubating 50 μl of lysate with 50 μl of luciferase assay substrate (Promega, E1501) according to the manufacturer’s instructions [39,40]. All studies with infectious pseudotyped particles

(11) Virus-based IFNγ gene delivery and photodynamic therapy cooperate to remodel the tumor microenvironment and suppress breast cancerMolecular Therapy OncologyMarch 19, 2026Ksenija Korotkaja, Zhanna Rudevica, Darija Lapina, Dace Skrastina, Juris Jansons, Olga Nilova, Karina Spunde, Anna Zajakinaof SFV/Luc was assessed based on the luciferase activity measured with a luciferase assay (Cat. No. E1501; Promega, Madison, WI, USA). For the luciferase assay (Cat. No. E1501; Promega, Madison, WI, USA), the

(12) CXCR4-tropic HIV-1 infection in an immunocompetent monkey modelNature CommunicationsMarch 9, 2026Nicholas R. Meyerson, Vanessa L. Bauer, Will T. Fattor, Cody J. Warren, Arturo Barbachano-Guerrero, Melisa J. Weiss, Obaiah Dirasantha, Bridget L. Burris, Adetunji S. Adesina, Joseph L. Timpona, et al.temperature for 15 min. Luciferase signal was activated using the Promega luciferase assay system (Promega #E1501) and detected using an Agilent BioTek Synergy HTX multi-mode microplate reader (Agilent #S1LFA). We

(13) A multi-glycoprotein synergized recombinant virus system resolved the research dilemma of Epstein-Barr virus entry and facilitated the development of entry-specific drugs or vaccinesCurrent Research in Microbial SciencesMarch 9, 2026Tianyu Lu, Shufeng Tian, Jiapeng Luo, Zhe Zhou, Yanying Liang, Wei Xu, Shenglei Yu, Lingyun Shao, Huang Huang, Lu Lu, et al.infection of EBV-pNL4-3 was similar, and its infection was measured by Luciferase Assay System (Promega, E1501). 2.4 Entry-inhibition assay based on rEBV-VSV For the entry-inhibition assay evaluating neutralizing

(14) A small‑molecule HDAC/PDE modulator activates human adipocyte UCP1 and resolves inflammatory signalingJ Transl MedMarch 5, 2026Mayyasa Rammah, Atilio Reyes Romero, Najeeb Halabi, Sirin Abuaqel, Andrea Guennoun, Alexander Dömling, Jingxuan Shan, Lotfi Chouchanewas added for 24 hours, followed by a luciferase assay using the Luciferase Assay System (Promega, E1501) according to the manufacturer’s protocol. Luciferase activity was measured using a CLARIOstar plate

(15) Cardiomyocyte-derived TGFB3 attenuates cardiac fibrosis and preserves cardiac function in heart failureScientific ReportsMarch 2, 2026Jizhong Xuan, Jiayu Zhou, Yuanji Huang, Mengyu Huang, Shengyan Pu, Liang Fang, Yuxuan Chen, Jieyuan Xue(R&D Systems, 8420-B3) for 16 h. Luciferase activity was determined using the Luciferase Assay System (Promega, E1501) following the manufacturer’s instructions. Relative luciferase activity was calculated as the luminescence

(16) Targeting Melanoma-Associated Fibroblasts to Overcome Cancer Stem Cell-Driven Drug ResistanceJ Am Coll SurgFebruary 23, 2026Hongwei Shao, Mecker Moller, Nga Le, Yulexi Y Ortiz, Omaida C Velazquez, Zhao-Jun Liubovine serum. Cells were stably transduced with DsRed (Clontech, 632496) or firefly luciferase (Promega, E1501). Cells were maintained in DMEM with 10% fetal bovine serum and 1% penicillin-streptomycin. Three-dimensional

(17) Targeted capture and cloning of Rps6 reveal redundancy in soybean resistance genesBMC Plant BiologyFebruary 17, 2026Yanick Asselin, Adèle Deshaies, Lesley Milnes, Sangeeta Parthasarathy, Parthasarathy Santhanam, James Houghton, Vanessa Tremblay, Caroline Labbé, François Belzile, Kamil Witek, et al.al. [40], using Promega cell lysis solution (catalogue number E1531) and luciferin (catalogue number E1501), in a standard white 96-well plate. Measurements were taken using a ThermoFisher Scientific Varioskan

(18) Env-antibody coevolution identifies B cell priming as the principal bottleneck to HIV V2 apex broadly neutralizing antibody developmentSci ImmunolFebruary 13, 2026Rumi Habib, Ryan S. Roark, Hui Li, Andrew Jesse Connell, Michael P. Hogarty, Kshitij Wagh, Shuyi Wang, Lorie Marchitto, Ashwin N. Skelly, John W. Carey, et al.luciferase activity levels were measured using the Promega luciferase assay system (Promega, catalog no. E1501) on a BioTek Synergy Neo2 plate reader (Agilent Technologies). Neutralization IC50/ID50 values were

(19) Rapidly acquired HIV-1 neutralization breadth in a rhesus V2 apex knockin mouse model after a single bolus immunizationSci ImmunolFebruary 13, 2026Amrit Raj Ghosh, Rumi Habib, Nitesh Mishra, Ryan S. Roark, Madhav Akauliya, Ali A. Albowaidey, Joel D. Allen, Khaled Amereh, Gabriel Avillion, Maria Bottermann, et al.PBS and luciferase activity levels measured using the Promega luciferase assay system (Promega Cat# E1501) on a BioTek Synergy Neo2 plate reader (Agilent Technologies). Neutralization IC50 and ID50 values were

(20) The kinetics and transmission-blocking dynamics of P. falciparum sexual-stage antibody responses in a six-year cohort of Ugandan children and adultsResearch SquareFebruary 10, 2026Sara Lynn Bousema, Sara Lynn Nayebare, Patience Nayabare, Jonathan Briese, Gerine Nijman, Karina Teelen, Geert-Jan van Gemert, Geert-Jan van van Gemert, Wouter Graumans, Catriona Patterson, et al.an by addition of luciferase assay substrate using a Luciferase assay kit (Luciferase assay system (E1501), Promega, USA). TRA of plasma samples was defined relative to the control groups in every SMFA. For

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3/15/2017Firefly Luciferase Assay Kit Nina FlerinWe are interested in enhancing the killing capacity of HIV-specific CD8 T cells by reengineering TCRs expressed by these cells. We evaluate TCRs cloned from CTLs of infected patients for their ability... Read Review
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2/9/2016Luciferase Assay System from Promega Haiquan LuWe used this kit for the measurement of luciferase activities after transfection with a luciferase reporter.

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Luciferase Assay System, 10-Pack

Luciferase Assay System, 10-Pack from Promega

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Promega

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Luciferase Assay System by Promega

Luciferase Assay System From Promega

Luciferase Assay System From Promega

Luciferase Assay System, 10-Pack from Promega

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