Luciferase Assay System, 10-Pack E1501 from Promega

Product Specs
ItemLuciferase Assay System, 10-Pack
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE1501
ReactivityMammalian, Bacteria, Plant
Detection TargetLuciferase
SpeciesMammalian. Bacteria,Plant
Quantity1,000 assays
SensitivityTo 10–20 moles of luciferase.
Assay Time5 minutes
MethodLuciferase
Sample TypeMammalian cell. Bacteria&,Plant cells

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Targeting Melanoma-Associated Fibroblasts to Overcome Cancer Stem Cell-Driven Drug ResistanceJournal of the American College of SurgeonsApril 1, 2026Hongwei Shao, Mecker Moller, Nga Le, Yulexi Y Ortiz, Omaida C Velazquez, Zhao-Jun Liubovine serum. Cells were stably transduced with DsRed (Clontech, 632496) or firefly luciferase (Promega, E1501). Cells were maintained in DMEM with 10% fetal bovine serum and 1% penicillin-streptomycin. Three-dimensional

(2) MMP14 is a safe target of CAR-T therapy against liver cancer and metastasisJ Transl MedMarch 26, 2026Ye Yu, Shirui Zou, Jiawei Fan, Dong Yang, Nan Liu, Zhiyong Bai, Yongjie Zhu, Lihong Li, Dan Cao, Xudong Zhao, et al.mixtures were incubated for 24 h. Luciferase activity was measured via a Luciferase Assay System (Promega, E1501). The release of TNF-α and IFN-γ in culture medium was measured via a specific ELISA kit in accordance

(3) Computational Design of Broad-Spectrum Ebola Antibodies through Framework and Complementarity-Determining Region Synergistic OptimizationResearch (Wash D C)March 23, 2026Xinhui Zhang, Xiuying Liu, Jingya Zhou, Peixiang Gao, Shengnan Pan, Xuehua Yang, Huarui Duan, Yi Liao, Fangyuan Zhang, Xuemeng Dong, et al.activity was measured by incubating 50 μl of lysate with 50 μl of luciferase assay substrate (Promega, E1501) according to the manufacturer’s instructions [39,40]. All studies with infectious pseudotyped particles

(4) Virus-based IFNγ gene delivery and photodynamic therapy cooperate to remodel the tumor microenvironment and suppress breast cancerMolecular Therapy OncologyMarch 19, 2026Ksenija Korotkaja, Zhanna Rudevica, Darija Lapina, Dace Skrastina, Juris Jansons, Olga Nilova, Karina Spunde, Anna Zajakinaof SFV/Luc was assessed based on the luciferase activity measured with a luciferase assay (Cat. No. E1501; Promega, Madison, WI, USA). For the luciferase assay (Cat. No. E1501; Promega, Madison, WI, USA), the

(5) A multi-glycoprotein synergized recombinant virus system resolved the research dilemma of Epstein-Barr virus entry and facilitated the development of entry-specific drugs or vaccinesCurrent Research in Microbial SciencesMarch 9, 2026Tianyu Lu, Shufeng Tian, Jiapeng Luo, Zhe Zhou, Yanying Liang, Wei Xu, Shenglei Yu, Lingyun Shao, Huang Huang, Lu Lu, et al.infection of EBV-pNL4-3 was similar, and its infection was measured by Luciferase Assay System (Promega, E1501). 2.4 Entry-inhibition assay based on rEBV-VSV For the entry-inhibition assay evaluating neutralizing

(6) CXCR4-tropic HIV-1 infection in an immunocompetent monkey modelNature CommunicationsMarch 9, 2026Nicholas R. Meyerson, Vanessa L. Bauer, Will T. Fattor, Cody J. Warren, Arturo Barbachano-Guerrero, Melisa J. Weiss, Obaiah Dirasantha, Bridget L. Burris, Adetunji S. Adesina, Joseph L. Timpona, et al.temperature for 15 min. Luciferase signal was activated using the Promega luciferase assay system (Promega #E1501) and detected using an Agilent BioTek Synergy HTX multi-mode microplate reader (Agilent #S1LFA). We

(7) A small‑molecule HDAC/PDE modulator activates human adipocyte UCP1 and resolves inflammatory signalingJ Transl MedMarch 5, 2026Mayyasa Rammah, Atilio Reyes Romero, Najeeb Halabi, Sirin Abuaqel, Andrea Guennoun, Alexander Dömling, Jingxuan Shan, Lotfi Chouchanewas added for 24 hours, followed by a luciferase assay using the Luciferase Assay System (Promega, E1501) according to the manufacturer’s protocol. Luciferase activity was measured using a CLARIOstar plate

(8) Cardiomyocyte-derived TGFB3 attenuates cardiac fibrosis and preserves cardiac function in heart failureScientific ReportsMarch 2, 2026Jizhong Xuan, Jiayu Zhou, Yuanji Huang, Mengyu Huang, Shengyan Pu, Liang Fang, Yuxuan Chen, Jieyuan Xue(R&D Systems, 8420-B3) for 16 h. Luciferase activity was determined using the Luciferase Assay System (Promega, E1501) following the manufacturer’s instructions. Relative luciferase activity was calculated as the luminescence

(9) Targeted capture and cloning of Rps6 reveal redundancy in soybean resistance genesBMC Plant BiologyFebruary 17, 2026Yanick Asselin, Adèle Deshaies, Lesley Milnes, Sangeeta Parthasarathy, Parthasarathy Santhanam, James Houghton, Vanessa Tremblay, Caroline Labbé, François Belzile, Kamil Witek, et al.al. [40], using Promega cell lysis solution (catalogue number E1531) and luciferin (catalogue number E1501), in a standard white 96-well plate. Measurements were taken using a ThermoFisher Scientific Varioskan

(10) Rapidly acquired HIV-1 neutralization breadth in a rhesus V2 apex knockin mouse model after a single bolus immunizationSci ImmunolFebruary 13, 2026Amrit Raj Ghosh, Rumi Habib, Nitesh Mishra, Ryan S. Roark, Madhav Akauliya, Ali A. Albowaidey, Joel D. Allen, Khaled Amereh, Gabriel Avillion, Maria Bottermann, et al.PBS and luciferase activity levels measured using the Promega luciferase assay system (Promega Cat# E1501) on a BioTek Synergy Neo2 plate reader (Agilent Technologies). Neutralization IC50 and ID50 values were

(11) Env-antibody coevolution identifies B cell priming as the principal bottleneck to HIV V2 apex broadly neutralizing antibody developmentSci ImmunolFebruary 13, 2026Rumi Habib, Ryan S. Roark, Hui Li, Andrew Jesse Connell, Michael P. Hogarty, Kshitij Wagh, Shuyi Wang, Lorie Marchitto, Ashwin N. Skelly, John W. Carey, et al.luciferase activity levels were measured using the Promega luciferase assay system (Promega, catalog no. E1501) on a BioTek Synergy Neo2 plate reader (Agilent Technologies). Neutralization IC50/ID50 values were

(12) Virus glycoprotein nanodisc platform for vaccine analyticsNature CommunicationsFebruary 10, 2026Kimmo Rantalainen, Alessia Liguori, Gabriel Ozorowski, Claudia Flynn, Jon M. Steichen, Olivia M. Swanson, Patrick J. Madden, Sabyasachi Baboo, Swastik Phulera, Anant Gharpure, et al.at RT. Neutralization was measured by adding 30 μl luciferase reagent (substrate) per well (Promega #E1501) and measuring luminescence. IC50 was calculated using a one-site Fit Log IC50 nonlinear regression

(13) The kinetics and transmission-blocking dynamics of P. falciparum sexual-stage antibody responses in a six-year cohort of Ugandan children and adultsResearch SquareFebruary 10, 2026Sara Lynn Bousema, Sara Lynn Nayebare, Patience Nayabare, Jonathan Briese, Gerine Nijman, Karina Teelen, Geert-Jan van Gemert, Geert-Jan van van Gemert, Wouter Graumans, Catriona Patterson, et al.an by addition of luciferase assay substrate using a Luciferase assay kit (Luciferase assay system (E1501), Promega, USA). TRA of plasma samples was defined relative to the control groups in every SMFA. For

(14) Impaired host shutoff is a fitness cost associated with baloxavir marboxil resistance mutations in influenza A virus PA/PA-X nuclease domainPLoS PathogFebruary 9, 2026Jack R. Case, Denys A. Khaperskyypost-transfection using CLARIOstar Plus imager (BMG Labtech, Cary, NC, USA) with Promega Luciferase Assay System (E1501, Promega, Madison, WI, USA) according to the manufacturer’s protocol. At least 3 independent biological

(15) ISX9 activates the Wnt/β-catenin signaling pathway and exerts neuroprotective effects in Alzheimer’s diseaseAlzheimer's Research & TherapyFebruary 5, 2026Jianhong Xiao, Yi Liu, Mingli Peng, Jiaying Ma, Shujing Lei, Yuexuan Chen, Shanshan Liu, Xibao Zhao, Desheng Lu, Qi Sunh. Cells were lysed and luciferase activity was measured using a luciferase assay kit (Promega, Cat#E1501, Madison, WI, USA). The luciferase values were standardized according to the difference in transfection efficiency

(16) Regulation of virion production by the ORF8 signal peptide across SARS-CoV-2 variantsbioRxivFebruary 3, 2026Mir M. Khalid, Irene P. Chen, Frank S. Soveg, Taha Y. Taha, Takako Tabata, Rahul K. Suryawanshi, Abdullah M. Syed, Alison Ciling, Maria McCavitt-Malvido, Ursula Schulze-Gahmen, et al.passive lysis buffer (E1941) for 15 minutes. After that, 50 μl of luciferase assay buffer (Promega, E1501) was added and luminescence was read using the TECAN plate reader (Infinite® 200 PRO) with auto attenuation,

(17) Origin of class B J-domain proteins involved in amyloid transactionsProceedings of the National Academy of SciencesJanuary 13, 2026Przemyslaw Domanski, Milena Stolarska, Katarzyna Kalinowska, Dominik Purzycki, Brenda A. Schilke, Hubert Wyszkowski, Marcin Pitek, Aneta Szymanska, Katarzyna Bury, Jacek Czub, et al.1.5 μM; Hsp105, 0.15 μM, and JDP, 1 μM. FLuc activity was measured using the Luciferase Assay Kit (E1501, Promega) with a GloMax® 20/20 Luminometer (Promega). Statistical analyses of chaperone activities were

(18) Modelling severe COVID-19 in TLR3-mutated hiPSCs-derived lung organoidsCell Death DiscoveryDecember 26, 2025Andrea Latini, Paola Spitalieri, Federica Centofanti, Barbara Rizzacasa, Donatella Amatore, Giorgia Grilli, Riccardo De Santis, Lorenzo Vaccaro, Vito Luigi Colona, Giulio Puleri, et al.quantitatively measured using the Promega Luciferase Assay System (Promega, Madison, Wisconsin, WI, USA, E1501), and luminescence was evaluated with a luminometer, following the manufacturer’s instructions. SARS-CoV-2

(19) Structural insights into cationic amino acid transport and viral receptor engagement by CAT1Nature CommunicationsDecember 18, 2025Lingyun Xia, Bingqian Lin, Rongfeng Zou, Yanyan Wu, Jiaying Xu, Yan Pan, Yuan Yuan, Shuo Li, Yang Yang, Xuemin Chencultured for another 48 h. Luciferase activity was measured using the Luciferase Assay System (Promega, E1501) on a Varioskan LUX plate reader (Thermo Fisher Scientific). All assays were performed in triplicate. Immunofluorescence

(20) Testing antimicrobial peptides inhibiting protein synthesis in living E. coli and K. pneumoniae using bio-orthogonal non-canonical amino-acid taggingFront MicrobiolDecember 16, 2025Luigi de Pascale, Agnese D’Amore, Adriana Di Stasi, Martino Morici, Sabrina Pacor, Alessandro Tossi, Thuy Duong Pham, Attilio Fabbretti, Daniel N. Wilson, Mario Mardirossian, et al.chimney plates). Finally, 40 μL of luciferase assay substrate solution were added to the wells (Promega, E1501), and the luminescence measured using a plate reader (Tecan Infinite 200 PRO). The negative control

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(1)

3/15/2017Firefly Luciferase Assay Kit Nina FlerinWe are interested in enhancing the killing capacity of HIV-specific CD8 T cells by reengineering TCRs expressed by these cells. We evaluate TCRs cloned from CTLs of infected patients for their ability... Read Review
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2/9/2016Luciferase Assay System from Promega Haiquan LuWe used this kit for the measurement of luciferase activities after transfection with a luciferase reporter.

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Luciferase Assay System, 10-Pack

Luciferase Assay System, 10-Pack from Promega

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Promega

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Luciferase Assay System by Promega

Luciferase Assay System From Promega

Luciferase Assay System From Promega

Luciferase Assay System, 10-Pack from Promega

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