Luciferase Assay System, 10-Pack E1501 from Promega

Product Specs
ItemLuciferase Assay System, 10-Pack
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE1501
ReactivityMammalian, Bacteria, Plant
Detection TargetLuciferase
SpeciesMammalian. Bacteria,Plant
Quantity1,000 assays
SensitivityTo 10–20 moles of luciferase.
Assay Time5 minutes
MethodLuciferase
Sample TypeMammalian cell. Bacteria&,Plant cells

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) A cell-impermeable kinase inhibitor uncovers outside-in signaling pathways that promote HIV-1 infectionJournal of VirologyJune 23, 2026Kelsey Vinzant, Natalia Cheshenko, Heng Pan, Ronald Cutler, Joshua N. Buckler, Andreas Luxenburger, Lawrence D. Harris, Jeffrey R. Johnson, Steven C. Almo, Betsy C. Heroldbuffer (Promega, #E1941), and luciferase activity was measured using the Promega assay system (Promega, #E1501). PBMCs (2 × 106 cells) were inoculated with 50 ng p24 in the presence of alkyl-CIMSS or DMSO. Every

(2) Alternol-Induced Oxidative Modification of SQSTM1/p62 Is Associated with Nrf2 Signaling and Autophagy-Related Responses in Prostate Cancer CellsAntioxidants (Basel)June 22, 2026Wang Liu, Jiang Zhao, Changlin Li, Haixia Xu, Ruibao Chen, Xing Zeng, Jun Yang, Cuncong Zhong, Xiangwei Wang, Benyi LiAuranofin, and rapamycin were obtained from Cayman Chemicals (Arbor, MI, USA). The Luciferase assay system (E1501) and TMR-conjugated Halo ligand (G8251) were purchased from Promega (Madison, WI, USA). Lyso-Tracker

(3) Quantitative limits of host-driven HIV transcription and host gene control by the viral transactivator TatNucleic Acids ResJune 22, 2026Chuan Li, Yuan Ma, Yi Wang, Huiming Yang, Susana T Valente(Promega, #E194A). Luciferase activity was quantified using the Luciferase Assay System (Promega, #E1501) on a Berthold luminometer and normalized to total protein content determined by Bradford assay (Bio-Rad

(4) Induction of broadly neutralizing HIV antibodies by a two-step mechanism informs vaccine designScienceJune 18, 2026Ashwin N. Skelly, Harry B. Gristick, Hui Li, Edem Gavor, Andrew J. Connell, Edward F. Kreider, Lorie Marchitto, Michael P. Hogarty, Maddy L. Newby, Joel D. Allen, et al.Triton X-100 in PBS and luciferase activity was quantified using a luciferase assay reagent (Promega E1501) and a BioTek Synergy Neo2 plate reader (Agilent). Animals were considered as having developed a bNAb

(5) Amelioration of Vascular Hyperpermeability Mediated by Targeted LNP Delivery of VE-Cadherin mRNA In VivoBlood AdvancesJune 9, 2026Lanfranco Leo, Tyler Ellis Papp, Chia-Ching Chen, Jennifer Pham, Soomin Jeong, Faris Halilovic, Jane Thompson, Awurama Akyianu, Jianhao Zeng, Panteleimon Rompolas, et al.detection with the Varioskan LUX Multimode Microplate Reader with the Luciferase Assay System (Promega, E1501), respectively. In vivo imaging system signals were quantified as photon flux using LivingImage software

(6) Effector Vδ1 γδ T cells mediate anti-tumor immunity and are reprogrammed by imatinib in human gastrointestinal stromal tumorResearchSquareJune 1, 2026Ronald DeMatteo, Jonathan H. Sussman, Jonathan Sussman, Montana Morris, Juan Esteban Perez, Jiazhen Perez, Katherine Tardy, Katherine Tardy, Hyunjee Hartlein, Shujing Hartlein, et al.for 24h. Cells were then lysed, and cytotoxicity was quantified using the Luciferase Assay System (E1501, Promega) on a GloMax Discover Microplate Reader, according to manufacturer’s instructions. For three-dimensional

(7) Lipid Nanoparticle Surface Engineering with Heparosan Polysaccharides for Safe and Effective mRNA Delivery In Vitro and In VivoACS Appl Mater InterfacesMay 27, 2026Yuxin He, Lin Wang, Rameswari Velayutham, Trisha I. Valerio, Samantha D. Ricketts, Yuanhong Sun, Anand C. Annan, James Bowman, Thao Tran, Mobina Mohammadnejad, et al.(5-moUTP) (APExBIO, R1016, Houston, TX, United States); Luciferase Assay System (Promega Products, E1501, Madison, WI, United States); d-luciferin potassium salt [Gold Biotechnology (U.S. Registration No 3,257,927)]. Cell Culture

(8) WAT-to-BAT communication facilitates the sustained activation of BAT thermogenesis during cold exposureCell DiscovMay 26, 2026Jieyuan Xue, Ding Chen, Chenfeng Wang, Jue Wang, Jiayu Zhou, Wenyan Li, Junxi Qian, Yuanji Huang, Liang Fang, Hongyong Song, et al.cells were washed with PBS. Luciferase activity was measured using a Luciferase Assay System (Promega, E1501) following the manufacturer’s instructions. Establishment of stable overexpression and knockdown cell

(9) Redirection of SARS-CoV-2 to phagocytes by intranasal sACE2-Fc as a universal decoy confers complete prophylactic protectionElifeMay 18, 2026Jingyi Wang, Jiangchuan Li, Alex WH Chin, Bin Luo, Junkang Wei, Jiale Qiu, Jianwei Ren, Yin Xia, Thomas Braun, Leo LM Poon, et al.cells in the presence of 8 µg/ml polybrene. Infectivity was assessed via a luciferase assay (Promega #E1501). Protein production and purification 293T and Expi293F cells were transfected with HDM-CMV-sACE2(-Fc)(-LALA)-his

(10) Chimeric autoantibody receptor T cells for targeted depletion of myeloperoxidase-specific anti-neutrophil cytoplasmic antibody-producing B cellsFront ImmunolMay 8, 2026Heather M. Sosnoski, William Aguilar, Shawna K. Brookens, David A. Degaramo, John T. Keane, Hyeon-Gyu S. Lewis, Damodar Gullipalli, Wenchao Song, Avery D. Poseyintracellular CBG luciferase. First, cells were washed and lysed with Reporter Lysis 5× Buffer (Promega, #E1501) and frozen at −80°C. Plates were thawed and 40 μl of supernatant combined with 50 μl of luciferase

(11) Allogeneic CRISPR-Engineered CAR-T Cells Drive Potent Antitumor Activity in Solid TumorsbioRxivMay 5, 2026Mingyu Huo, Dan Li, Nan Li, Alex Quan, Tianyuzhou Liang, Dan Henderson, Jason Sagert, Minh Pham, Luke Hanley, Kelly Maeng, et al.lysed by the passive lysis buffer (E1941, Promega) at room temperature. Add luciferase assay reagent (E1501, Promega) to each sample and then measure the light produced using the SpectraMax (Molecular Devices)

(12) Discovery and Preclinical Validation of a Clinically Optimized Mitochondrial Complex I Modulator for Alzheimer’s DiseasebioRxivApril 17, 2026Sergey Trushin, Thi Kim Oanh Nguyen, Mark Ostroot, Alexander Galkin, Toshihiko Nambara, Wenyan Lu, Takahisa Kanekiyo, Graham Johnson, Eugenia Trushinang/ml) for 6 h. NF-κB luciferase activity was measured using the Luciferase Assay System (Promega, E1501). n = 4 independent experiments. e C273 restores mitochondrial biogenesis, expression of ETC complexes,

(13) Hydroxylation of the HIV-1 antisense protein promotes immune evasion of HIV-1 via modulation of TBK1Nature CommunicationsApril 13, 2026Xinguang Lin, Wenrui Zhang, Manman Li, Chuanzhen Zhang, Jingna Xun, Wendong Han, Jun Chen, Yihua Zhang, Dapeng Yanmanufacturer’s protocol. Viral titers were determined in TZM-bl cells using a Luciferase Assay System (E1501, Promega), in accordance with the manufacturer’s instructions. Replication-competent HIV-1, HIV-1Δasp

(14) Targeting Melanoma-Associated Fibroblasts to Overcome Cancer Stem Cell-Driven Drug ResistanceJournal of the American College of SurgeonsApril 1, 2026Hongwei Shao, Mecker Moller, Nga Le, Yulexi Y Ortiz, Omaida C Velazquez, Zhao-Jun Liubovine serum. Cells were stably transduced with DsRed (Clontech, 632496) or firefly luciferase (Promega, E1501). Cells were maintained in DMEM with 10% fetal bovine serum and 1% penicillin-streptomycin. Three-dimensional

(15) ILC3s mediate intestinal immune-epithelial interactions via TGF-β1 activationMucosal ImmunologyApril 1, 2026Diana Coman, John W. Bassett, Isabelle Coales, Ainize Peña-Cearra, Emily Read, Emma H. Kromann, Daniel Brice, Zuzanna Łukasik, Helena Paidassi, Matthew R. Hepworth, et al.DMEM medium (Table 2). After 20 hours of incubation at 37 °C and 5 % CO2, a luciferase assay (Promega E1501) was performed and luminescence measured, for further details, see online supplemental materials and

(16) MMP14 is a safe target of CAR-T therapy against liver cancer and metastasisJ Transl MedMarch 26, 2026Ye Yu, Shirui Zou, Jiawei Fan, Dong Yang, Nan Liu, Zhiyong Bai, Yongjie Zhu, Lihong Li, Dan Cao, Xudong Zhao, et al.mixtures were incubated for 24 h. Luciferase activity was measured via a Luciferase Assay System (Promega, E1501). The release of TNF-α and IFN-γ in culture medium was measured via a specific ELISA kit in accordance

(17) Computational Design of Broad-Spectrum Ebola Antibodies through Framework and Complementarity-Determining Region Synergistic OptimizationResearch (Wash D C)March 23, 2026Xinhui Zhang, Xiuying Liu, Jingya Zhou, Peixiang Gao, Shengnan Pan, Xuehua Yang, Huarui Duan, Yi Liao, Fangyuan Zhang, Xuemeng Dong, et al.activity was measured by incubating 50 μl of lysate with 50 μl of luciferase assay substrate (Promega, E1501) according to the manufacturer’s instructions [39,40]. All studies with infectious pseudotyped particles

(18) Virus-based IFNγ gene delivery and photodynamic therapy cooperate to remodel the tumor microenvironment and suppress breast cancerMolecular Therapy OncologyMarch 19, 2026Ksenija Korotkaja, Zhanna Rudevica, Darija Lapina, Dace Skrastina, Juris Jansons, Olga Nilova, Karina Spunde, Anna Zajakinaof SFV/Luc was assessed based on the luciferase activity measured with a luciferase assay (Cat. No. E1501; Promega, Madison, WI, USA). For the luciferase assay (Cat. No. E1501; Promega, Madison, WI, USA), the

(19) CXCR4-tropic HIV-1 infection in an immunocompetent monkey modelNature CommunicationsMarch 9, 2026Nicholas R. Meyerson, Vanessa L. Bauer, Will T. Fattor, Cody J. Warren, Arturo Barbachano-Guerrero, Melisa J. Weiss, Obaiah Dirasantha, Bridget L. Burris, Adetunji S. Adesina, Joseph L. Timpona, et al.temperature for 15 min. Luciferase signal was activated using the Promega luciferase assay system (Promega #E1501) and detected using an Agilent BioTek Synergy HTX multi-mode microplate reader (Agilent #S1LFA). We

(20) A multi-glycoprotein synergized recombinant virus system resolved the research dilemma of Epstein-Barr virus entry and facilitated the development of entry-specific drugs or vaccinesCurrent Research in Microbial SciencesMarch 9, 2026Tianyu Lu, Shufeng Tian, Jiapeng Luo, Zhe Zhou, Yanying Liang, Wei Xu, Shenglei Yu, Lingyun Shao, Huang Huang, Lu Lu, et al.infection of EBV-pNL4-3 was similar, and its infection was measured by Luciferase Assay System (Promega, E1501). 2.4 Entry-inhibition assay based on rEBV-VSV For the entry-inhibition assay evaluating neutralizing

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3/15/2017Firefly Luciferase Assay Kit Nina FlerinWe are interested in enhancing the killing capacity of HIV-specific CD8 T cells by reengineering TCRs expressed by these cells. We evaluate TCRs cloned from CTLs of infected patients for their ability... Read Review
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2/9/2016Luciferase Assay System from Promega Haiquan LuWe used this kit for the measurement of luciferase activities after transfection with a luciferase reporter.

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Luciferase Assay System, 10-Pack

Luciferase Assay System, 10-Pack from Promega

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Promega

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Luciferase Assay System by Promega

Luciferase Assay System From Promega

Luciferase Assay System From Promega

Luciferase Assay System, 10-Pack from Promega

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