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Luciferase Assay System, 10-Pack
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Luciferase Assay System, 10-Pack
Luciferase Assay System, 10-Pack
Luciferase Assay System, 10-Pack from Promega
(2)
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Citations:
(572)
Figures:
(3)
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Promega for
Luciferase Assay System, 10-Pack
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Description
An extremely sensitive reagent for rapid quantitation of firefly luciferase. Can be used for assays in single-tube luminometers or in multiwell-plate luminometers with auto-injectors
Product Specs
Company Info
Citations
Reviews
Product Reviews
Product Specs
Item
Luciferase Assay System, 10-Pack
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
E1501
Reactivity
Mammalian, Bacteria, Plant
Detection Target
Luciferase
Species
Mammalian. Bacteria,Plant
Quantity
1,000 assays
Sensitivity
To 10–20 moles of luciferase.
Assay Time
5 minutes
Method
Luciferase
Sample Type
Mammalian cell. Bacteria&,Plant cells
Add to Compare List
Promega
2800 Woods Hollow Rd
Madison
,
WI
53711
United States
Phone
: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516
Email: custserv@promega.com
techserv@promega.com
Company Profile
Website:
http://www.promega.com
Citations (572)
(1)
Segmented poly(A) tails with microRNA target sites confer tissue-specific regulation for mRNA therapeutics
Molecular Therapy Nucleic Acids
December 8, 2026
Rui Qi, Hua Chen, Rui Chen, Hui Bao, Ying Sun, Ruiwen Xu, Lu Han, Yingmei Xu, Juan Li, Na Li,
et al.
performed according to the manufacturer’s instructions of
Luciferase Assay System
kit (
Promega
, Cat. #
E1501
) and
luminescence
signal was measured using Centro Microplate Luminometer (Bethold). Luciferase
assay
(2)
Monitoring Py8119 breast cancer cell proliferation in direct cocultures with osteoblasts and osteoclasts
Journal of Biological Chemistry
November 1, 2026
Yanmei Yang, Bin Wang
Viability/Cytotoxicity assay kit (Cat. No. L32250) were purchased from
Invitrogen
.
Luciferase
detection kit (Cat. No.
E1501
) was obtained
Promega
. Anti-mouse RANKL-neutralizing monoclonal antibody (RANKL-Ab, Cat. No.
BE0191
)
(3)
Structural basis for broad orthopoxvirus neutralization by two antibodies targeting distinct L1R epitopes
Cell Reports Medicine
October 20, 2026
Li Chen, Guanying Zhang, Haotian Lin, Yue Zhang, Jingfei Li, Linfeng Shu, Xiaolin Wang, Ruihua Li, Fangxin Zhao, Xiaofan Zhao,
et al.
,
ThermoFisher Scientific
,Cat#
23227
Expi293™ Expression System Kit
,
ThermoFisher Scientific
,Cat#
A14635
Luciferase Assay System
,
Promega
,Cat#
E1501
RNeasy Mini Kit,
Qiagen
,Cat#
74104
One-step QuantiNova Probe
RT-qPCR
Kit,
Qiagen
,Cat#
208354
Genomic
(4)
A Cilia–Centered Nicotinamide Adenine Dinucleotide (NAD +) Signaling Network Regulates Hypothalamic Lysosomal Homeostasis and Metabolic Aging
Aging Cell
October 20, 2026
Se Eun Park, Eun Roh, Jae Woo Park, Seong Jun Kim, So Yeon Jeong, Chae Beom Park, Hyo Sun Lim, Ji Soo Kim, Mun Gyeom Kim, Myeong Ju Kim,
et al.
indicated. After 24–48 h, luciferase activity was measured using a
luciferase reporter assay
(
Promega
, #
E1501
), and values were normalized to β–galactosidase activity to adjust for
transfection efficiency
. Results
(5)
Protocol for scalable production of orally deliverable R-spondin1-loaded small extracellular vesicles for WNT-signaling activation and tissue regeneration
STAR Protoc
October 8, 2026
Lingyan Yang, Ting Jiang, Shixiang Wang, Yue Yang, Zhiping Qiao, Yun-Shen Chan
commercial
assays
,Critical commercial
assays
,, Dual-Luciferase Report
Assay
System,
Promega
,Cat.
E1501
RSPO1
enzyme-linked immunosorbent assay
kit,
R&D Systems
,Cat.
DY4645-05
High
Sensitivity
ECL Substrate
(6)
Deletions in variable region 1 of the HIV envelope accelerate V3-glycan bNAb induction in SHIV-infected rhesus macaques
Proceedings of the National Academy of Sciences
October 6, 2026
Michael P. Hogarty, Hui Li, Chengyan Zhao, Christian L. Martella, Ashwin N. Skelly, Rumi Habib, Andrew Jesse Connell, Frederic Bibollet-Ruche, Weimin Liu, John W. Carey,
et al.
Triton X-100 in PBS and luciferase activity was quantified using a luciferase
assay
reagent (
Promega
,
E1501
) and a
BioTek
Synergy Neo2
plate reader (Agilent). The same 16 heterologous
virus
screening
panel was
(7)
Lipid Nanoparticle Surface Engineering with Heparosan Polysaccharides Enhances mRNA Efficacy via Reduced Endosomal Retention
ACS Nano
October 6, 2026
Yuxin He, Lin Wang, Abigael P. Williams, Trisha I. Valerio, Yuanhong Sun, James Bowman, Samantha D. Ricketts, Savannah Hammond, Aidan Sison, Jezan Alexandre,
et al.
(5-moUTP) (APExBIO, R1016, United States, Houston, Texas).
Luciferase Assay System
(
Promega
Products,
E1501
, United States, Madison, Wisconsin). D-Luciferin, Potassium Salt (Gold Biotechnology (U.S. Registration
(8)
Synergistic antitumor immune activation in melanoma using LA-PegPI polymeric gene vaccine in conjunction with PD-1 inhibition
iScience
September 18, 2026
WeiKe Li, Ling Liu, XiaoXuan Yao, Manzama-Esso Abi, ChunLei Ge, DongYan Bian, XiaoHui Ding, XiaoRui Pang, RuiLei Li, JiaXing Cheng,
et al.
μL lysis buffer, and luciferase activity was measured using
Promega
’s luciferase
assay
system (Cat#
E1501
) according to the manufacturer’s instructions. Protein concentration was determined by BCA
assay
(Beyotime
(9)
RFC5 and STAT3 form a transcriptional complex to drive NSCLC progression via c-Myc
iScience
September 18, 2026
Jinhua Yan, Tao Yi, Wentao Tian, Jinzheng Wu, Rongji Yu, Shumiao Zhang, Zekun Cheng, Xiaotang Di, Hao Jiang, Doudou Wen,
et al.
were lysed, and luciferase activities were measured by the luciferase
assay
system kit(
Promega
, Cat#
E1501
, China) following the manufacturer’s standard instructions. Each experiment was repeated three times. Quantification
(10)
Discovery and preclinical validation of a translationally optimized mitochondrial complex I modulator for Alzheimer's disease
NPJ Drug Discov
September 2, 2026
Sergey Trushin, Thi Kim Oanh Nguyen, Mark Ostroot, Jarred J. Nesbitt, Tetiana Kovalenko, Alexander Galkin, Toshihiko Nambara, Wenyan Lu, Takahisa Kanekiyo, Graham Johnson,
et al.
ng/ml) for 6 h. NF-κB luciferase activity was measured using the
Luciferase Assay System
(
Promega
,
E1501
), n = 4 independent experiments. e C273 restores mitochondrial biogenesis, expression of ETC complexes,
(11)
NF-κB-dependent and independent inflammatory responses during acute HIV-1 infection in a model of human microglia
Front Immunol
August 14, 2026
Aracelly Gaete-Argel, Catarina Ananías-Sáez, Cecilia Rojas-Fuentes, Sebastian Giraldo-Ocampo, Daniela Jara, Tomás Hernández-Díaz, Camila Ortega-Orellana, Delia López-Palma, Hisashi Akiyama, Suryaram Gummuluru,
et al.
Firefly luciferase activity was measured using 25 μL of the
Luciferase Assay System
substrate (Cat
E1501
,
Promega
). 2.3
Pseudotyped virus
production, quantification and
infections
Plasmids used to produce
(12)
The transmission-blocking dynamics of Plasmodium falciparum sexual-stage antibody responses in a six-year cohort of Ugandan children and adults
Nature Communications
August 13, 2026
Sara Lynn Blanken, Patience Nayebare, Jonathan Briese, Gerine Nijman, Karina Teelen, Rianne Stoter, Geert-Jan van Gemert, Wouter Graumans, Tate Oulton, Catriona Patterson,
et al.
and by addition of luciferase
assay
substrate using a Luciferase assay kit (
Luciferase assay system
(
E1501
),
Promega
, USA). TRA of plasma samples was defined relative to the
control groups
in every SMFA. For
(13)
Vaccination elicits HIV broadly neutralizing antibodies in primates
Nature
June 30, 2026
Jon M. Steichen, Patrick J. Madden, Claudia T. Flynn, Swastik Phulera, Monolina Shil, Oleksandr Kalyuzhniy, Alessia Liguori, Carolyne Kifude, Leigh M. Sewall, Christopher A. Cottrell,
et al.
temperature. Neutralization was measured by adding 30 μl luciferase reagent (substrate) per well (
Promega
,
E1501
) and measuring the
luminescence
. ID50 or
IC50
values were calculated using a one-site fit (log[
IC50
])
(14)
Lead Optimization of TgCDPK1 Inhibitors for the Treatment of Toxoplasmosis
Journal of Medicinal Chemistry
June 25, 2026
Michael P. Mannino, Anusha Gokanapalle, Shrushti Patil, Anoopjit Singh Kooner, Chhuttan Lal Meena, Matthew Medcalf, Igi Vilza, Jennifer Barks, Yong Fu, Jing Xia,
et al.
replicate for 72 h and luciferase activity was monitored using the
Luciferase Assay System
(
Promega
,
E1501
). Plates were read with a Cytation3 Imaging Multimode plate reader (Biotek), as described previously.27
(15)
A cell-impermeable kinase inhibitor uncovers outside-in signaling pathways that promote HIV-1 infection
Journal of Virology
June 23, 2026
Kelsey Vinzant, Natalia Cheshenko, Heng Pan, Ronald Cutler, Joshua N. Buckler, Andreas Luxenburger, Lawrence D. Harris, Jeffrey R. Johnson, Steven C. Almo, Betsy C. Herold
buffer (
Promega
, #
E1941
), and luciferase activity was measured using the Promega
assay
system (
Promega
, #
E1501
). PBMCs (2 × 106 cells) were inoculated with 50 ng p24 in the presence of alkyl-CIMSS or DMSO. Every
(16)
Mutant KRAS-driven selective mRNA translation reveals mechanisms and therapeutic vulnerabilities in cancer
Cell Reports
June 23, 2026
Ankita Shrivastava, Eric Nels Pederson, Trang Uyen Nguyen, Jacky Chuen, Prathibha Mohan, Shivangi Pande, Ana Ruiz Toribio, Nicolas Lecomte, Jerry Melchor, John C. McAuliffe,
et al.
was measured following indicated drug treatment using the
Luciferase Reporter Assay
System (
Promega
E1501
), following the manufacturer’s instructions. QUANTIFICATION AND STATISTICAL ANALYSIS Statistical
(17)
Alternol-Induced Oxidative Modification of SQSTM1/p62 Is Associated with Nrf2 Signaling and Autophagy-Related Responses in Prostate Cancer Cells
Antioxidants (Basel)
June 22, 2026
Wang Liu, Jiang Zhao, Changlin Li, Haixia Xu, Ruibao Chen, Xing Zeng, Jun Yang, Cuncong Zhong, Xiangwei Wang, Benyi Li
Auranofin
, and rapamycin were obtained from
Cayman Chemicals
(Arbor, MI, USA). The
Luciferase assay system
(
E1501
) and TMR-conjugated Halo ligand (
G8251
) were purchased from
Promega
(Madison, WI, USA). Lyso-Tracker
(18)
Quantitative limits of host-driven HIV transcription and host gene control by the viral transactivator Tat
Nucleic Acids Res
June 22, 2026
Chuan Li, Yuan Ma, Yi Wang, Huiming Yang, Susana T Valente
(Promega, #E194A). Luciferase activity was quantified using the
Luciferase Assay System
(
Promega
, #
E1501
) on a Berthold luminometer and normalized to total protein content determined by Bradford
assay
(
Bio-Rad
(19)
Induction of broadly neutralizing HIV antibodies by a two-step mechanism informs vaccine design
Science
June 18, 2026
Ashwin N. Skelly, Harry B. Gristick, Hui Li, Edem Gavor, Andrew J. Connell, Edward F. Kreider, Lorie Marchitto, Michael P. Hogarty, Maddy L. Newby, Joel D. Allen,
et al.
Triton X-100 in PBS and luciferase activity was quantified using a luciferase
assay
reagent (
Promega
E1501
) and a
BioTek
Synergy Neo2
plate reader (Agilent). Animals were considered as having developed a bNAb
(20)
Development of bispecific antibodies with enhanced neutralization activity against tested SARS-CoV-2 Omicron subvariants
Frontiers in Immunology
June 17, 2026
Ching-Hsuan Hsu, Ting-Yi Chang, Ya-Min Chang, Shin-Han Lin, Tzu-Ning Chen, Keng-Hao Hsu, Jhong-Jhe You
cells were lysed and luciferase activity was measured using the
Luciferase Assay System
(
Promega
, #
E1501
) on a
Biotek
Synergy LX
Multi-Mode Reader. The percentage of neutralization was calculated as the reduction
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Reviews
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(1)
3/15/2017
Firefly Luciferase Assay Kit
Nina Flerin
We are interested in enhancing the killing capacity of HIV-specific CD8 T cells by reengineering TCRs expressed by these cells. We evaluate TCRs cloned from CTLs of infected patients for their ability...
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(1)
2/9/2016
Luciferase Assay System from Promega
Haiquan Lu
We used this kit for the measurement of luciferase activities after transfection with a luciferase reporter.
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Luciferase Assay System by Promega
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Luciferase Assay System, 10-Pack from Promega
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