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Dual-Glo Luciferase Assay System
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Dual-Glo Luciferase Assay System
Dual-Glo Luciferase Assay System
Dual-Glo Luciferase Assay System from Promega
(1)
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Citations:
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Figures:
(3)
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Dual-Glo Luciferase Assay System
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Description
A homogeneous reagent system that enables fast, simple quantitation of both firefly and Renilla luciferase luminescence signals from cells that have not been preconditioned or prelysed
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Dual-Glo Luciferase Assay System
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
E2920
Reactivity
Mammalian
Detection Target
Luciferase
Species
Mammalian
Sample Type
Mammalian cell culture media
Method
Luciferase
Quantity
10ml
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Promega
2800 Woods Hollow Rd
Madison
,
WI
53711
United States
Phone
: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516
Email: custserv@promega.com
techserv@promega.com
Company Profile
Website:
http://www.promega.com
Citations (1023)
(1)
The mRNA architecture of the translation termination site primes programmed stop codon readthrough events in Drosophila
RNA Biol
December 31, 2026
Laveena Kansara, Georg Wolfstetter, Daniel Wintermayr, Ioannis Alexopoulos, Alejandra Escos, Marc Friedländer, Ylva Engström
assay
Luciferase
assays
were performed using the Dual-Glo®
Luciferase Reporter Assay
System (
Promega
, #
E2920
) following the manufacturer’s instructions.
Luminescence
was measured with an EnSpire 2300 Multi-mode
(2)
Microsomal triglyceride transfer protein restricts steroid production in Leydig cells by regulating SREBP2
iScience
June 19, 2026
Atrayee Chattopadhyay, Munichandra Babu Babu Tirumalasetty, Thomas Palaia, Bhargavi Gangula, Rachel Ruoff, Qing Robert Miao, M. Mahmood Hussain
A12216
CellTiter 96® AQueous One Solution,
Promega
,Cat#
G3582
Dual-Glo® Luciferase Assay System
,
Promega
,Cat#
E2920
Progesterone ELISA Kit,
Cayman Chemical
,Cat#
582601
Testosterone ELISA Kit,
Cayman Chemical
,Cat#
582701
Pierce BCA Protein Assay Kit
(3)
Rare Truncating HAND2 Variants Predispose to Atrial Fibrillation
Genes (Basel)
June 17, 2026
Hong Zhang, Xiao-Qing Hu, Ning Li, Dao-Liang Zhang, Xing-Biao Qiu, Chen-Xi Yang, Ying-Jia Xu, Yi-Qing Yang, Juan Wang
dual-luciferase activities were successively analyzed by exploiting a dual-
reporter assay
kit (Cat.#
E2920
;
Promega
, Madison, WI, USA). The activities of the HCN4 and NPPA promoters were shown as the ratios
(4)
miR-151-5p regulates neural stem cell fate by targeting APH1A to modulate Notch signaling gradients
Stem Cell Reports
June 9, 2026
Xinrun Wang, Li Li, Zhuo Chen, Yi Zeng, Pengcheng Shu, Lin Hou, Bin Yin, Wei Liu, Xiaozhong Peng
DP214
EndoFree Plasmid Maxi Kit (10
),
QIAGEN
,Cat#
12362
Dual-Glo Luciferase Assay System
,
Promega
,Cat#
E2920
,, Deposited data,Deposited data,Deposited data ,,
RNA-seq
Raw and analyzed data,This paper,GEO:GSE327779 ,,
Experimental models
(5)
A multiplex hextuple luciferase assay for advancing cellular signaling analysis in plant cells
Plant Communications
June 8, 2026
Shanshan Song, Saloni Darji, Nathaniel Carter, Koen J.T. Venken, Santiago Navarro
All
luminescence measurements
were performed using the
Dual-Glo® Luciferase Assay System
(
Promega
,
E2920
). After overnight (∼18 h) post-transformation
incubation
, 35 μl of
protoplast
suspension (∼8000 cells)
(6)
Disrupting Notch signalling by a small molecule inhibiting dihydroorotate dehydrogenase activity
Sci Rep
June 6, 2026
Eike-Benjamin Braune, Dirk Wienke, Anita Seshire, Timo Heinrich, Martin Haraldsson, Sonia Lain, Urban Lendahl
clones
. Luciferase activity was assessed using the dual-GLO luciferase detection kit from
Promega
(cat #
E2920
), following the manufacturer’s instructions. A minimum of two technical replicates were measured per
(7)
Integrative genomics and single-cell CRISPRi screening dissect Alzheimer’s GWAS non-coding variants regulating TSPAN14
The American Journal of Human Genetics
June 4, 2026
Shannon Laub, Natalia Tulina, Matthew Hoffman, Joe Bormeh Faryean, Sandhya Ramachandran, Khanh Trang, Stephanie M. Lewkiewicz, Alessandra Chesi
Nano-Glo Dual-luciferase reporter (
Promega
, cat#
N1610
) and Dual-Glo luciferase reporter (
Promega
, cat#
E2920
) kits. These
assays
rely on the simultaneous expression of two distinct luciferase-encoding plasmids,
(8)
The hsa_circ_0070354/miR-4322/RAB3B Axis Facilitates the Carcinogenesis and Development of Non-Small Cell Lung Cancer (NSCLC)
Cancer Med
June 2, 2026
Yuejiao Huang, Shiyi Qin, Yu Zhang, Xinliang Gu, Ming Zheng, Guojie Ling, Lei Yang, Shaoqing Ju
California, USA).
Luciferase
activity was measured 48 h post‐
transfection
using a Dual‐
Luciferase
Kit (Cat.
E2920
,
Promega
, Wisconsin, USA), normalized to
Renilla
activity. 4.8 Xenograft Model BALB/c nude
mice
(4–5
(9)
Synthesis and Development of Novel Small-Molecule MEIS2 Inhibitors That Induce Cell Death in Breast Cancer Cells by Targeting the Homeobox Domain
Pharmaceuticals (Basel)
June 1, 2026
Fatih Kocabaş, Birkan Girgin, Merve Uslu, Pınar Siyah, Arif Mermer
activity was measured using the
Dual-Glo Luciferase Assay System
(
Promega
, Madison, WI, USA, Cat. No.
E2920
) on a
Luminoskan Ascent Microplate Luminometer
(
Thermo
Lab System, Helsinki, Finland). Firefly luciferase
(10)
The cooperative regulation of miR ‐221 by APE1 and AUF1 impacts p27 Kip1 defining a miR signature relevant for cervical cancer
FEBS Open Bio
June 1, 2026
Matilde Clarissa Malfatti, Nicolò Gualandi, Gianluca Tell, Giulia Antoniali
activity was measured 24 h after
transfection
using the Dual‐GLO
luciferase reporter assay
system (
E2920
;
Promega
, Madison, WI, USA). RNA immunoprecipitation (RIP)
HeLa cells
were seeded in 10‐cm plates
(11)
Protease-activated receptor 1 as an endogenous model of peptidergic Gαq-Gα12-biased G protein signaling
Frontiers in Molecular Biosciences
May 25, 2026
Braden S. Fallon, Robert A. Campbell, Justin G. English
on a
PHERAstar FSX
(BMG Labtech, RRID:SCR_027001) using the Dual-Glo Luciferase
Assay
(
Promega
, Cat#
E2920
) according to the manufacturer’s instructions.
Firefly
luminescence
was normalized to
Renilla
luminescence
(12)
KIF5A downregulation in spinal muscular atrophy links axonal regeneration defects with ALS
JCI Insight
May 22, 2026
Tetsuya Akiyama, Yi Zeng, Caiwei Guo, Olivia Gautier, Lauren Koepke, Heankel Lyons, Elana Molotsky, Juliane S. Bombosch, Odilia Sianto, Jay P. Ross,
et al.
normalized to Renilla luciferase activity using
Nano-Glo Dual-Luciferase Reporter Assay System
(
Promega
E2920
).
SMN
truncation and mutant constructs. A series of
SMN
truncation and mutant constructs was generated
(13)
Redundant and distinct mechanisms suppress innate immune activation during SARS-CoV-2 infection
PLoS Biol
May 20, 2026
Fuchun Zhou, Sivakumar Periasamy, Nathaniel D. Jackson, Wan Sze Cheng, Ruben Soto Acosta, Aarti Tripathi, Kritika Kedarinath, Philipp A. Ilinykh, Chengjin Ye, Shailendra Chauhan,
et al.
after the treatment, the
reporter assay
was performed using Dual-Glo Luciferase System (
Promega
, Cat#
E2920
) according to the manufacturer’s instructions. The
luminescence
in the wells of the 96-well plates was
(14)
Myeloid Mas drives pyruvate kinase M2-mediated Spi1 lactylation to fuel inflammatory senescence in MASLD
Signal Transduct Target Ther
May 19, 2026
Luying Zhao, Shujing Xu, Shikai Qiao, Zhe Wang, Shenglan Wang, Chun Liu, Shuo Zhang, Peng Wang, Xianghua Sun, Shanshan Li,
et al.
additional 24 h. Luciferase activity was quantified using a dual-
luciferase reporter assay
system (
Promega
,
E2920
, Madison, WI, USA), with Renilla luciferase activity serving as an internal normalization control.
Flow cytometry
(15)
Reciprocal regulation between autism risk gene POGZ and circadian clock
JCI Insight
May 8, 2026
Ting Wu, Jiao He, Chu-Jun Xu, Chi-Yu Li, Pingchuan Zhang, Yanfeng Wang, Shanshan Zhu, Lusi Zhang, Jingtan Zhu, Jing Zhang,
et al.
luciferase reporter assay
Dual luciferase reporter
assays
were performed following the manufacturer’s protocol (
E2920
,
Promega
). In brief, cells in 24-well plates were
transfected
with 10 ng luciferase reporter and 1 ng
(16)
HDAC7-Induced Epigenetic Repression Modulates ATF3 Functional Plasticity in Colorectal Cancer Pathogenesis
Int J Biol Sci
May 1, 2026
Qi Wang, Donglei Ji, Yanjie Jia, Shuang Li, Wenjing Gao, Tingting Liang, Yingying Liang, Caroline Zeng, Chunyu Wang, Ka Lung Cheung,
et al.
luciferase activities were measured sequentially using the
Dual-Glo Luciferase Assay System
(
Promega
,
E2920
) on a GloMax Navigator luminometer. Relative promoter activity was calculated as the ratio of
Firefly
(17)
IRE1–XBP1driven induction of TMED9 stabilizes ATF6 during ER stress to promote cell survival
Cellular & Molecular Biology Letters
April 30, 2026
Chen Lenchisky, Areen Muhammad Majadly, Irena Bronshtein Berger, Danielle Biton, Alaa Daoud Sarsour, Narkis Arbeli, Tamar Cohen, Naama Amos, Sara Kinstlinger, Ortal Cohen,
et al.
luciferase activity was quantified using the Dual-Glo®
Dual-Luciferase® Reporter Assay System
(
Promega
,
E2920
) on a
BioTek
Synergy H1
reader. An equal volume (50 µl) of Dual-Glo® Reagent was added per well. Then,
(18)
New Insights into the Development of Papillary Thyroid Cancer: The Roles of miR-1179 and ELF3
Cells
April 29, 2026
Nicolas Henry, Nisrine Bahassou, Frédérick Libert, Geneviève Dom, Carine Maenhaut
Renilla
and Firefly luciferase activities were quantified with the
Dual-Glo® Luciferase Assay System
(
E2920
,
Promega
). Each experiment was performed in at least three independent biological replicates, and for
(19)
Emergence and evolution of chimeric pseudogenes (φgenes) contribute to genetic and functional diversity of the human genome
Nucleic Acids Res
April 28, 2026
Amruta R Jadhav, Sushmita Sahoo, Yash L Kuber, Krutika Hatwar, Avinash Varanwal, Khushi Tanwar, Shubhankar Palwankar, Gauri Chatrasal, Avinash M Mali, Sharmila A Bapat
assays
were performed using the
Dual-Glo™ Luciferase Assay System
per manufacturer’s protocol (
Promega
, #
E2920
).
Immunofluorescence
staining
Twenty thousand cells/coverslip (CV1 EV, CV1 RA OE, OVCAR3 EV, and OVCAR3
(20)
MiR-551b-5p deficiency associates with malignant phenotypes in gastric cancer: potential mediation by TRAF6
BMC Gastroenterology
April 27, 2026
Luyao Wang, Chunhua Ni, Peichen Xia, Ying Liu, Tianchi Zhuang, Ting Xu, Danyang Wang, Hongkai Yu, Yingqi Yang, Xiaomeng Jiang
luciferase activity was measured using the
Dual-Glo® Luciferase Assay System
(
Promega Corporation
; cat. no.
E2920
).
Western blotting
Washed cells were lysed using cold lysis buffer containing
protease inhibitors
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Reviews
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(1)
12/27/2021
Great and Efficient Luciferase System
Wei Yang Sit
The convenient "add-mix-measure" protocol generates signals from cells that have not been preconditioned or prelysed.
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Dual-Glo Luciferase Assay System from Promega
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