Dual-Glo Luciferase Assay System E2920 from Promega

Product Specs
ItemDual-Glo Luciferase Assay System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE2920
Quantity10ml
ReactivityMammalian
Detection TargetLuciferase
Sample TypeMammalian cell culture media
MethodLuciferase
SpeciesMammalian

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) The mRNA architecture of the translation termination site primes programmed stop codon readthrough events in DrosophilaRNA BiolDecember 31, 2026Laveena Kansara, Georg Wolfstetter, Daniel Wintermayr, Ioannis Alexopoulos, Alejandra Escos, Marc Friedländer, Ylva Engströmassay Luciferase assays were performed using the Dual-Glo® Luciferase Reporter Assay System (Promega, #E2920) following the manufacturer’s instructions. Luminescence was measured with an EnSpire 2300 Multi-mode

(2) Myeloid Mas drives pyruvate kinase M2-mediated Spi1 lactylation to fuel inflammatory senescence in MASLDSignal Transduct Target TherMay 19, 2026Luying Zhao, Shujing Xu, Shikai Qiao, Zhe Wang, Shenglan Wang, Chun Liu, Shuo Zhang, Peng Wang, Xianghua Sun, Shanshan Li, et al.additional 24 h. Luciferase activity was quantified using a dual-luciferase reporter assay system (Promega, E2920, Madison, WI, USA), with Renilla luciferase activity serving as an internal normalization control. Flow cytometry

(3) Emergence and evolution of chimeric pseudogenes (φgenes) contribute to genetic and functional diversity of the human genomeNucleic Acids ResApril 28, 2026Amruta R Jadhav, Sushmita Sahoo, Yash L Kuber, Krutika Hatwar, Avinash Varanwal, Khushi Tanwar, Shubhankar Palwankar, Gauri Chatrasal, Avinash M Mali, Sharmila A Bapatassays were performed using the Dual-Glo™ Luciferase Assay System per manufacturer’s protocol (Promega, #E2920). Immunofluorescence staining Twenty thousand cells/coverslip (CV1 EV, CV1 RA OE, OVCAR3 EV, and OVCAR3

(4) ADAR1 and ADAR2 associate with the RNA exosome and modulate RNA stabilityNucleic Acids ResApril 24, 2026Dragana Vukić, Qiupei Du, Anna Cherian, Damiano Amoruso, Květoslava Brožinová, Ludivine Wacheul, Valentina Lacovich, Christiane Zorbas, Leena Yadav, Jiří Sedmík, et al.-luciferase reporter assay Firefly and Renilla luciferase activities were measured with Dual-Glo® Luciferase Assay System (Promega, #E2920) according to the manufacturer’s instructions. Firefly activity was normalized to Renilla activity within

(5) A microRNA expression signature in infant t(4;11) KMT2A::AFF1+ BCP-ALL uncovers novel therapeutic targetsHemasphereApril 23, 2026Camille Malouf, Alasdair Duguid, Kirsten S. Vrenken, Tom Leah, Ragini Medhi, Giuseppina Camiolo, Leslie Nitsche, Hélène Jakobczyk, Rishi S. Kotecha, Richard A. Anderson, et al.Renilla luciferase activity were measured using the Dual‐Glo Luciferase Assay System (Promega Cat# E2920) and a GloMax Discover MicroPlate Reader (Promega Cat# GM3000) according to the manufacturer's instructions. Bioinformatic analysis

(6) TBL1X/TBL1XR1 govern β-cell identity through a PAX6-containing gene regulatory networkNat CommunApril 23, 2026Alina A. Walth-Hummel, Celine Jouffe, Peter Weber, Karsten Motzler, Julia Geppert, Michael Sterr, Wei Gan, Iwona Szczerbinska, Ann-Christine König, Stefanie M. Hauck, et al.production. Luciferase activity was assayed using the Dual-glo luciferase assay system (Promega, Cat# E2920). For this, INS1E cells were transfected with control siRNA or siRNA against TBL1X and TBL1XR1 for 72

(7) Improved functional JAG1 and NOTCH2 variant testing in patients with clinical or suspected Alagille syndrome using new low-Notch activity cellsHum GenetApril 18, 2026Nicole Buhl, Eva-Doreen Pfister, Daniel V. Oliveira, Fabio Turetti, Eberhard Lurz, Ulrich Baumann, Nataliya Di Donato, Thomas Illig, Britta Skawran, Emma R. Andersson, et al.signal-receiving cells. Luciferase activity was measured at 48 h using the Dual-Glo system (Promega #E2920). For HEK293T-LNA cell assays, cells (1.25 × 10⁵/well in 24-well plates) were transfected (Lipofectamine

(8) A Novel Mechanism for Depot-Specific Leptin Gene Expression Regulation and its Persistence After Weight LossiScienceApril 17, 2026Natalie Taege, Jan Hendric Britsemmer, Andreas Israel, Christin Krause, Sina Junge, Annette Feuchtinger, Siegfried Ussar, Paul Thomas Pfluger, Timo Gemoll, Sonja Charlotte Schriever, et al.assays,Critical commercial assays,Critical commercial assays ,, DUAL-GLO Luciferase Assay,"Promega, US",E2920 EpiTect Fast DNA Bisulfite Kit,QIAGEN; Netherlands,59802 Formalin 10% neutral buffered,"Sigma-Aldrich,

(9) KIAA1429 promotes clear cell renal cell carcinoma progression by regulating MYC mRNA stabilityiScienceApril 17, 2026Xu Zhu, Cheng Shen, Yong Zhang, Xinfeng Chen, Wei Zhang, Bing Zheng, Zhan ChenRNA-Binding Protein Immunoprecipitation Ki,Millipore,Cat#17-700 Dual-Glo Luciferase Assay System,Promega,Cat#E2920 ,, Deposited data,Deposited data,Deposited data ,, "GEO database (GSE36895, GSE53757, GSE40435)",NCBI

(10) The CCCH-tandem zinc-finger protein OsTZF1 positively regulates basal immunity but suppresses Piz-t-mediated blast resistance in ricePlant CommunicationsApril 13, 2026Xiaoman You, Jiawei Long, Ruyi Wang, Xinyi Hou, Jisong Wang, Xuanda Huang, Min Wang, Xiao Xu, Feng He, Huiting Hu, et al.transformation, and luciferase activity was measured using the Dual-Luciferase Reporter Assay System (E2920, Promega). The GFP or GUS-HA plasmid was used as a negative control. Primers are given in Supplemental

(11) Selective vulnerability of cerebral vasculature to NOTCH3 variants in small vessel disease and rescue by phosphodiesterase-5 inhibitorSci AdvApril 3, 2026Xiangjun Zhao, Chaowen Yu, Antony Adamson, Aite Zhao, Huiyu Zhou, Pankaj Sharma, Tao WangLuciferase reporter assay The activation of NOTCH signaling pathway was detected by Dual-Glo Luciferase Assay System (Promega; E2920) as we previously described (14). IPSC-derived VSMCs were plated into a 12-well plate and transfected

(12) A Double-Negative Prostate Cancer Subtype is Vulnerable to SWI/SNF-Targeting Degrader MoleculesCancer ResearchApril 2, 2026Phillip Thienger, Irene Paassen, Xiaosai Yao, Philip D. Rubin, Marika Lehner, Nicholas Lillis, Andrej Benjak, Sagar R. Shah, Alden King-Yung. Leung, Simone de Brot, et al.Renilla internal control signal were detected using the Dual-Glo Luciferase Assay system (Promega, E2920). Luminescence was read using the Varioskan LUX plate reader (Thermo Fisher Scientific, RRID: SCR_026792)

(13) A multi-stage computational pipeline and in vitro validation for the discovery of small-molecule translation inhibitors targeting the bacterial ribosomeRSC AdvApril 1, 2026Merve Yuce, Ezgi Koman, Fethiye Aylin Sungur, Ayten Yazgan-Karatas, Ozge Kurkcuoglureaction. Luciferase expression was measured using the Dual-Glo® Luciferase Assay System (Promega, Cat. No. E2920). For this, 10 µL of each reaction was transferred to a 384-well flat-bottom white plate and mixed with

(14) ENDOU-1-induced cytoplasmic HnRNPA3 recognizes m6A methylation on the upstream reading frame of human CHOP transcripts to achieve maximal CHOP translationCellular and Molecular Life SciencesMarch 28, 2026Hung-Chieh Lee, Yi-Hsin Huang, Chi-Cheng Hsieh, Yi-Nan Ke, Huai-Jen Tsaiinstructions. Luciferase (luc) activity was measured using the Dual-Glo luciferase assay kit (Promega, E2920). All siRNAs were designed and synthesized by Santa Cruz. The siRNAs used in this study were HnRNPA3

(15) MEG3 in glioma stem cells promotes glioblastoma angiogenesis through FUBP3-mediated VGF expressionClinical and Experimental MedicineMarch 23, 2026Yuan Li, Yujie Li, Jiaxin Tang, Xue Kong, Sibai Sun, Wei Wang, Haibo Wudual-luciferase reporter assay was conducted using the Dual-Glo® Luciferase Assay System (Promega, E2920) according to the manufacturer’s protocol, and luminescence intensity was measured using a LumiStation

(16) miR-181d coordinates homologous recombination and anti-tumor immune responses in glioblastomaiScienceMarch 20, 2026Gatikrushna Singh, Shilpi Singh, Iteeshree Mohapatra, Jay Hou, Andrew Ni, Debashis Barik, Haoyi Zheng, Stefan Kim, Mayur Sharma, Sean Lawler, et al.assays,Critical chemical assays,Critical chemical assays ,, Dual-Glo® Luciferase Assay System,Promega,E2920 miRNeasy Kit,Qiagen,217084 RNeasy Kit,Qiagen,74182 OmniScript RT Kit,Qiagen,205113 miRCURY LNA RT

(17) Cytoplasmic tail diversity determines the effector bias of the adhesion GPCR ADGRL2Cell Chem BiolMarch 19, 2026Krassimira A. Garbett, Chen Zheng, Julia Drube, Carsten Hoffmann, Vsevolod V. Gurevich, Richard C. Sandostimulation. Luciferase activity was measured using the Dual-Glo® Luciferase Assay System (Promega, Cat# E2920). 50 μL of Dual-Glo Luciferase reagent was added to the 50 μL of media remaining in each well, mixed,

(18) Exosomal Long Noncoding RNA H19 as a Biomarker and Therapeutic Target in Atrial FibrillationInt J Biol SciMarch 17, 2026Ji-Young Kang, Dasom Mun, Malgeum Park, Gyeongseo Yoo, Nuri Yun, Boyoung Jounghours after transfection, luciferase activity was measured using Dual-Glo Luciferase Assay System (E2920; Promega) according to the manufacturer's protocol. Pull-down assay with biotinylated miRNAs HEK293 cells

(19) Cell type-specific enhancers regulate IL-22 expression in innate and adaptive type 3 lymphoid cellsNature CommunicationsMarch 14, 2026Ankita Saini, Leone S. Hopkins, Vanida A. Serna, Matthew V. D. McCullen, Nicholas G. Selner, Bishan Bhattarai, José L. Fachi, Rebecca A. Glynn, Katharina E. Hayer, Craig H. Bassing, et al.nucleofection. Harvested cells were assayed in a 96-well plate using Dual-Glo Luciferase Assay System (Promega; E2920) following the manufacturer’s instructions. Splenic naïve CD4+ T cells from Catch22 mice were ex vivo

(20) microRNA-422a promotes HIV replication and innate immune evasion by targeting MECP2Molecular Therapy Nucleic AcidsMarch 12, 2026Li Du, Jean-Noël Billaud, Sushama Telwatte, Nikhila Kadiyala, Prerna Dabral, Mohamed S. Bouzidi, John Guatelli, Steven A. Yukl, Satish K. PillaiAfter 24 h, luciferase activity was measured using the Dual-Luciferase Reporter Assay Kit (Promega, #E2920) according to the manufacturer’s protocol. Flow cytometry HIV-1NL4-3-infected cells were fixed and

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12/27/2021Great and Efficient Luciferase System Wei Yang SitThe convenient "add-mix-measure" protocol generates signals from cells that have not been preconditioned or prelysed.

Dual-Glo Luciferase Assay System

Dual-Glo Luciferase Assay System from Promega

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Dual-Glo Luciferase Assay System from Promega

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