Luciferase Assay System E1500 from Promega

Product Specs
ItemLuciferase Assay System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE1500
ReactivityMammalian, Bacteria, Plant
SpeciesMammalian,Bacteria,Plant
Detection TargetLuciferase
Sample Typemammalian cells.,plant and bacterial cells,
MethodLuciferase
SensitivityTo 10^{–20
moles of luciferase.}
Assay TimePerform cell lysis, sample preparation and assays in as little as 5 minutes.
Quantity100 assays

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) A MYB-Related Transcriptional Module Cooperatively Represses Xylem Development in PoplarPlant Biotechnol JAugust 21, 2026Gaofeng Fan, Jiahui Jiang, Zihan Cheng, Feixiang Ma, Zhanling Sun, Zelin Li, Yu Qi, Guanzheng Qu, Tingbo Jiangsystem (Shanghai, China). Quantitative LUC activity was measured using the Luciferase Assay System (E1500, Promega) according to the manufacturer's instructions, with three biological replicates and three technical

(2) Esketamine Alleviates Neuropathic Pain and Inflammation via miR-153-3p/AKT3 in CCI RatsPain Res ManagAugust 17, 2026Xiumei Zhu, Yawen ZhangDual‐Luciferase Reporter Analysis Wild‐type and mutant AKT3 3′UTR sequences were cloned into pmirGL3 (Promega, E1500) to generate luciferase reporter constructs. They were co‐transfected with miR‐153–3p mimic (miR‐mimic),

(3) Targeting the αvβ5 Integrin Modifies the TGF-β-Rich Tumor Microenvironment of Pancreatic CancerCancer ResAugust 4, 2026Kodai Suzuki, Yuki Kunisada, Yukihito Kuroda, Hotaka Kawai, Tatiana Hurtado de Mendoza, Evangeline S. Mose, Shingo Eikawa, Henri Havia, Tero A. H. Järvinen, Caisheng Lu, et al.CA). After 6 hours of culture at 37°C, the cells were lysed, mixed with Luciferase Assay Reagent (Cat# E1500; Promega), and analyzed for luminescence intensity using Varioskan LUX (RRID: SCR_026792; Thermo Fisher

(4) Sentinel plants enable quantitative monitoring of bioavailable nitrate in soils and microbial environmentsProc Natl Acad Sci U S AJuly 28, 2026Eugene Li, Chiara Berruto, Tufan M. Oz, Elisa Grillo, Catherine Griffin, Yunqing Wang, Kimberley T. Muchenje, Jolie W. Jones, Gozde S. DemirerPromega; GeNL 520 to 590 nm, NLuc 430 to 485 nm) and the ELuc/RedF pair with the Luciferase Assay System (E1500, Promega; ELuc 490 to 545 nm, RedF 610 to 700 nm); signals were resolved by spectral deconvolution (Promega

(5) MPT0E028, a pan-HDAC inhibitor, ameliorates bleomycin-induced pulmonary fibrosis by promoting AT2-to-AT1 differentiation through the ATM/AMPK/FoxO1 pathwayJ Biomed SciJuly 21, 2026Chia-Hao Liu, Hong-Sheng Lee, Jing-Ping Liou, Wun-Hao Cheng, Hung-Sheng Hua, Bing-Chang Chen, Chien-Huang Lin0.3 μM) for an additional 24 h. Luciferase activity was measured using the Luciferase assay system (E1500, Promega, Madison, WI, USA), and the results were normalized to the expression levels of LacZ. Immunoprecipitation (IP

(6) GPCRs interaction with LGR5: Implications to cancer stemnessJournal of CancerJuly 20, 2026Jeetendra K Nag, Ganesh Subedi, Tatyana Rudina, Rachel Bar-Shavitwere washed, lysed and then Luciferase assay was performed with the Luciferase Reporter System (Cat# E1500; Promega, Heidelberg, Germany) according to the manufacturer's instructions, and luminescence was detected

(7) Activation of EZH2 Promoter Is Mediated by MAPK Signaling Regulated Transcription Factors in Anaplastic Thyroid CancerInt J Mol SciJuly 18, 2026Marcella Maringolo Cristovão, Diego Claro de Mello, Edna Teruko Kimura, Cesar Seigi Fuziwaratransfection, the cell lysate was collected using 1× Passive Lysis Buffer (Promega, Madison, WI, USA, cat. E1500) and luminescence was measured using Dual Luciferase® Reporter Assay kit (Promega) in the luminometer

(8) Synthesis and preliminary evaluation of novel compounds that demonstrate broad host-directed anti-leishmanial activityPLoS Negl Trop DisJuly 13, 2026Elizabeth G. Gurysh, M. Shamim Hasan Zahid, Monica M. Johnson, Antonio Landavazo, Ojas A. Namjoshi, Joseph W. Wilson, Devika M. Varma, Ryan N. Woodring, Aaron T. Hendricksen, Alexandra M. Lopez, et al.of the amastigotes within macrophages was determined using Promega firefly luminescence assay (Cat E1500) or Pierce Renilla luciferase assay (Cat 16166) for L. donovani and L. mexicana strains, respectively.

(9) PCSK5 promotes angiogenesis and cardiac repair after myocardial infarctionNat CommunJuly 3, 2026Jieyu Guo, Siyu Ma, Jinghua Ma, Ming Liu, Fei Ge, Xinhong Wang, Jiayi Lin, Nan Jiang, Xiaoke Lin, Zhipeng Lian, et al.harvested, and luciferase activity was measured with a Luciferase Assay Kit (Promega, Madison, WI, #E1500). β-galactosidase (β-gal). Relative Luc activity was calculated as the ratio of Luc/β-gal activity. Molecular docking

(10) GRHAL1, a novel lncRNA, regulates HIV-1 gene expression by modulating Tat- and Sp1-mediated HIV-1 LTR activationJ Gen VirolJuly 2, 2026Mohammad Ishaq, Heather Marshall, Ven Natarajancell type, and luciferase activity was quantitated using the Luciferase Assay System (Promega, Cat. # E1500). The luciferase activity was normalized to total protein concentration in the cell lysates using the

(11) N4-Acetylcytidine enhances synthetic mRNA translation yield and fidelityNatureJuly 1, 2026Sarah Schiffers, Blake W. Nelson, Maria Prigge, Shriya Krishna, Leslie Watkins, Yining Zhu, Nishu Tyagi, Hamid Beiki, Sudipto Das, Ayush Raman, et al.protein analysis were washed twice with PBS, scraped into PBS and lysed in cell culture lysis buffer (Promega, E1500) supplemented with protease and phosphatase inhibitor. To generate dsRNA-treated controls, HeLa cells

(12) Discovery of 5’ NAD capped viral RNAs reveals evolutionary divergent 5’ metabolite capping across hepacivirusesNature CommunicationsJune 29, 2026Helena S. Larsen, Anna V. Sherwood, Lizandro R. Rivera-Rangel, Line A. Ryberg, Paula H. Faller, Caroline E. Thorselius, Cathrine B. Vågbø, Kenn Holmbeck, Sonja Fernbach, Sabina Chubanava, et al.cells, luciferase expression in cell lysates was measured using the Luciferase Assay System (Promega E1500) on a CLARIOStar Plus microplate reader, the following manufacturer’s instructions. Cell viability assay

(13) A subunit vaccine for multiple respiratory virusesSci AdvJune 26, 2026Qinzhe Li, Wei-Chiao Huang, Sara H. Mahmoud, Chengjin Ye, Zachary R. Sia, Yiting Song, Yang Jiao, Yuan Luo, Shiqi Zhou, Reuben M. Pul, et al.incubation, the cells were then washed with PBS and lysed with 30 μl of lysis buffer (Promega, catalog no. E1500) for 10 min at room temperature with gentle shaking. Luciferase substrate (100 μl; CentroPRO catalog

(14) Preclinical evaluation of cysteine protease-inhibitor aloxistatin (E64d) for heart failure therapyJ Mol Med (Berl)June 25, 2026Maria Jordan, Angelika Stucki-Koch, Maximilian Schinke, Lena Willmer, Ingrid Senne, Naisam Abbas, Marco Bentele, Dana Oldenkott, Nico Lachmann, Linda Feldbrügge, et al.4 °C and 300 × g. Supernatant was removed, pellets were lysed in 70 µL Cell Culture Lysis Reagent (E1500, Luciferase Assay System, Promega, Walldorf, Germany) on ice for 15 min and cleared by centrifuge at

(15) MAP3K1 integrates RhoA/ROCK signaling to regulate epithelial morphogenesisiScienceJune 19, 2026Bo Xiao, Maureen Mongan, Young-Bum Kim, Ying Xiawere lysed, and luciferase activity was measured using the Luciferase Assay System (Promega, Cat. #E1500; Madison, WI, USA) following the manufacturer’s instructions. Luminescence was determined by a SpectraMax M5

(16) Non-viral targeted integration at the CISH locus enables CAR-NK cell engineering with enhanced anti-tumor activityMolecular Therapy OncologyJune 18, 2026Jiao Wang, Yao Sun, Jakob Starzyk, Fei Wang, Xin Dong, Richard Shan, Xuemei He, Keqiang Xie, Guozhu Xie, Hao Wuco-culture, relative luciferase activities were measured using the Promega Luciferase Assay System (Cat# E1500) according to the manufacturer’s instructions. Specific lysis of each group was calculated using the

(17) B7 homolog 3-targeted CAR-T cells secreting EGFR T-cell engagers for improved control of glioblastoma progressionMol BiomedJune 16, 2026Zongliang Zhang, Nian Yang, Hui Zeng, Yongdong Chen, Huaqing Lu, Long Xu, Zeng Wang, Guoqing Wang, Liangxue Zhou, Aiping Tongwere combined with 100 μL of CAR‑T supernatant. At the end of the incubation, a luciferase assay kit (#E1500, Promega) was used to lyse the cells, and luminescence was quantified on a BioTek Synergy H4 hybrid

(18) KLF6 activation marks an angiogenic and apoptosis resistant endothelial phenotype in pulmonary arterial hypertensionCommun BiolJune 15, 2026Rehab Alharbi, Merve Keles, Nadia Fernandes, Hannah Maude, Adam Fellows, Richard D. Williams, Chien-Nien Chen, Nathalie Lambie, Nik Matthews, May Al-Sahaf, et al.either normoxic or hypoxic conditions for 24 h. A luciferase reporter assay (Promega, USA, Cat. No. E1500) was then performed according to the manufacturer’s instructions. The intensity of luminescence, proportional

(19) Surface-driven endocrine activity of nanoplastics: polymer- and size-dependent estrogen and androgen receptors modulation without steroidogenesis perturbationParticle and Fibre ToxicologyJune 10, 2026Nikolina Peranić, Lucija Božičević, Nikolina Kalčec, Korinna Altmann, Jana Hildebrandt, Carlos Cuestas Ayllon, Raquel Portela, Jesús M. de la Fuente, Miguel A. Bañares, Ivana Vinković VrčekPBS. The cell lysate was prepared as suggested in the protocol for Promega luciferase assay system (E1500, Promega, Madison, WI, USA) by adding 20 µL of cell culture lysis reagent diluted 5 times with distilled

(20) SnRK2.6-mediated phosphorylation stabilizes proteasome regulator 1 (PTRE1) to enhance 26S proteasome activity and coordinate ABA signaling in ArabidopsisPlant CommunicationsJune 8, 2026Peng-Chao Hao, Ling-Li Zheng, Jin-Shuang Dou, Hong-Wei Xueharvested for lysis. LUC activity was measured using 20 μL of lysate and 100 μL of LUC assay reagent (E1500, Promega) with a luminometer (Varioskan Flash microplate reader, Thermo Scientific). GUS activity was

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2/9/2016Luciferase Assay System from Promega Haiquan LuWe used this kit for the measurement of luciferase activities after transfection with a luciferase reporter.

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Luciferase Assay System

Luciferase Assay System from Promega

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Promega

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Luciferase Assay System by Promega

Luciferase Assay System From Promega

Luciferase Assay System From Promega

Luciferase Assay System from Promega

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