Luciferase Assay System E1500 from Promega

Product Specs
ItemLuciferase Assay System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE1500
Quantity100 assays
ReactivityMammalian, Bacteria, Plant
Sample Typemammalian cells.,plant and bacterial cells,
MethodLuciferase
SensitivityTo 10^{–20
moles of luciferase.}
SpeciesMammalian,Bacteria,Plant
Detection TargetLuciferase
Assay TimePerform cell lysis, sample preparation and assays in as little as 5 minutes.

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) MAP3K1 integrates RhoA/ROCK signaling to regulate epithelial morphogenesisiScienceJune 19, 2026Bo Xiao, Maureen Mongan, Young-Bum Kim, Ying Xiawere lysed, and luciferase activity was measured using the Luciferase Assay System (Promega, Cat. #E1500; Madison, WI, USA) following the manufacturer’s instructions. Luminescence was determined by a SpectraMax M5

(2) Non-viral targeted integration at the CISH locus enables CAR-NK cell engineering with enhanced anti-tumor activityMolecular Therapy OncologyJune 18, 2026Jiao Wang, Yao Sun, Jakob Starzyk, Fei Wang, Xin Dong, Richard Shan, Xuemei He, Keqiang Xie, Guozhu Xie, Hao Wuco-culture, relative luciferase activities were measured using the Promega Luciferase Assay System (Cat# E1500) according to the manufacturer’s instructions. Specific lysis of each group was calculated using the

(3) Dual site targeting of the bacterial 70S ribosome by tetracyclinesNat CommunMay 19, 2026Swapnil C. Devarkar, Ivan B. Lomakin, Jimin Wang, Ayman Grada, Christopher G. Bunickwas detected in 1–2 min after addition of 50 μl of the Luciferase Assay Reagent (Promega, USA, cat. # E1500) using multimode plate reader TriStar LB941 (Berthold technologies LTD, UK) and analyzed using Prism

(4) PTBP1 is upregulated in response to Zika virus infection and restrains viral replication by hijacking viral NS1 protein to induce NS1 degradationJournal of VirologyMay 19, 2026Menglan Rao, Yue Kong, Shuang Liu, Zicong Chen, Jiuxiu Lin, Yicong Liang, Zhiwei Lei, Zhen LuoLuciferase reporter assay Luciferase reporter assay was processed by using Firefly Luciferase Reporter Assay System (Cat #E1500) (Promega) according to the manufacturer’s instructions. A549 cells were seeded in 24-well plates and

(5) Nuclear factor IX promotes endometriosis progression through transcriptional activation of tetraspanin-2J Mol Med (Berl)May 16, 2026Yuqi Liu, Qing Xue, Jingwen Zhu, Cheng Zeng, Xin Li, Jing Shang, Jie Li, Peili Wuinternal control. Cells were gathered 48 h post transfection, and the luciferase assay system (Promega, E1500) was used to measure the activity of luciferase. The activity of firefly luciferase was standardized

(6) Multimodal profiling reveals cell type-specific pseudouridine modification and density-dependent translational regulationNucleic Acids ResApril 21, 2026Caroline A McCormick, Michele Meseonznik, Yuchen Qiu, Oleksandra Fanari, Priyanka Goyal, Mitchell Thomas, Mina Shokoufandeh, Yifang Liu, Dylan Bloch, Cole Greenfield, et al.placed on ice to stop translation. To measure protein abundance, the Luciferase Assay System (Promega, E1500) was used. 2.5 μl of each Fluc RRL sample was added to separate wells. Fifty microliters of the luciferase

(7) Targeting DNA Polymerase Epsilon Induces Tumor Clearance and Activates an NF-κB-Mediated Inflammatory Response in Triple Negative Breast CancerCancer ResApril 21, 2026Elizabeth F. Sher, Kenji M. Fujihara, Anthony Tao, Paul Sastourne-Haletou, Diana Erenburg, Vladislav O. Sviderskiy, Hannan Mir, Triantafyllia Karakousi, Cynthia A. Loomis, Jiehui Deng, et al.Polyethylenimine (Polysciences, Cat# 239662) was from Polysciences. Luciferase Assay System kit (Promega, Cat# E1500) was from Promega. Lentiviral shRNAs were obtained from the The RNAi Consortium (TRC) collection of

(8) VAP1 promotes cardiac fibrosis by enabling PDGFR signaling in myofibroblastsExperimental & Molecular MedicineApril 20, 2026Shan Huang, Qianwen Zhao, Tinghui Shao, Chenghao Zhu, Yujia Xue, Naxia Chen, Yue Zhang, Huihui Xu, Ming Kong, Rui Wangtransfection. Reporter assay was performed with a luciferase assay system, as per vendor's instructions (E1500, Promega). Protein extraction, immunoprecipitation, and western blotting Whole-cell/tissue lysates

(9) Inhalable Perfluorocarbon RNA Nanocapsules Bypass Immune Clearance While Targeting Lung Epithelial and Lung Tumor CellsbioRxivApril 12, 2026Kasturi Siddhanta, Atefehsadat Monirvaghefi, Aditya Sundar, Braeden R. Pinkerton, Neha Kumari, Ling Ding, C. J. Woslager, Marjina Akter Kalpana, Chinmay M. Jogdeo, Ashley R. Ravnholdt, et al.buffer, and luciferase activity was quantified using the Promega Luciferase Assay System (Cat. No. E1500). CXCR4 inhibition assay: CXCR4 inhibitory activity by PAMD-C@PFOB was assessed by CXCR4-redistribution

(10) Genetic and pharmaceutical manipulation of H3K9 methyltransferase Suv39h1 promotes liver regeneration by unleashing HMGB2 transcriptionExperimental & Molecular MedicineApril 10, 2026Yunjie Lu, Jiawen Zhou, Xiulian Miao, Sheng Zeng, Lei Qin, Yong Xu, Shuai Wang, Zilong Liactivities were assayed at 24–48 h after transfection using a luciferase reporter assay system (Promega, E1500) as previously described28. Recombinant murine HGF (R&D, 2207-HG) was reconstituted in sterile PBS and

(11) The COX2-PGE2-PKA Axis Suppresses Antiviral Immunity by Inhibiting mtDNA-Dependent STING ActivationbioRxivApril 6, 2026Pham Thuy Tien Vo, Julien Cicero, Zichen Wang, Hiroyuki Hakozaki, Thomas S Hoang, Sendi Rafael Adame-Garcia, Dana J. Ramms, Kuniaki Sato, Erica Stevenson, Yuan Zhou, et al.figure legends. Luciferase assays were performed with the Firefly Luciferase Assay System (Promega, E1500) according to the manufacturer’s instructions. RNA extraction, cDNA synthesis and quantitative real-time PCR

(12) Sodium- myo -inositol cotransporter-1, SMIT1, promotes cardiac hypertrophy and fibrosis induced by pressure overload in miceCardiovascular ResearchMarch 26, 2026Alice Marino, Julien Cumps, Laura Guilbert, Angéline Geiser, Claire Baufays, Audrey Ginion, Laura Ferté, Sylvain Battault, Fiona De Matos, Jerome Ambroise, et al.NFAT-luciferase assay Luciferase activity was measured in supernatants using the Luciferase Assay System (LAR, E1500, Promega) following the manufacturer’s instruction. Briefly, 20 µL of cell lysate (800 000 cells/well

(13) Disrupting Akt-Wnt/β-catenin signaling suppresses glioblastoma stem cell growth and tumor progression in immunocompetent miceJ NeurooncolMarch 14, 2026Md Marzan Sarkar, Nathan Gonsalves, Amin Davarzani, Emma Mitchell, Amar M. Singh, Lohitash Karumbaiah, Steven L. Sticeeach well of a 96-well white opaque plate. 50µL Luciferase Assay Reagent II (LARII, Promega, Cat. No.: E1500) was added, and luminescence signals were detected immediately using the microplate reader. Luciferase

(14) Universal strategy to generate lentiviral vectors encoding lethal proteinsMolecular Therapy AdvancesMarch 12, 2026Benjamin Versier, Aravindhan Soundiramourty, Enzo Manchon, Philippe Ravassard, Dominique Charron, Yves Christen, Jacques Mallet, Reem Al-Daccak, Che SergueraFirefly Luciferase activity was quantified in lysate using the Luciferase Assay System kit (Promega, E1500). Luminescence (Luc assay) and absorbance (BCA assay) were measured on Spectramax i3X (Molecular Devices).

(15) METTL14/IGF2BP1 m 6 A axis promotes pyroptosis in Streptococcus pneumoniae -induced pneumonia by regulating NEK7 mRNA stabilityInfection and ImmunityMarch 10, 2026Cheng Chen, Di ZhangAfter 48 h, the cells were harvested. Luciferase activity was measured using a luciferase assay kit (#E1500; Promega, Madison, WI, USA), and relative luciferase activity was calculated by normalizing to an internal

(16) Inhalable perfluorocarbon RNA nanocapsules overcome biological barriers to treat lung metastasesJournal of Controlled ReleaseMarch 10, 2026Kasturi Siddhanta, Neha Kumari, Aditya Sundar, Atefehsadat Monirvaghefi, Braeden R. Pinkerton, Ling Ding, C. J. Woslager, Marjina Akter Kalpana, Chinmay M. Jogdeo, Ashley R. Ravnholdt, et al.buffer, and luciferase activity was quantified using the Promega Luciferase Assay System (Cat. No. E1500). 2.11 CXCR4 inhibition assay: CXCR4 inhibitory activity by PAMD-C@PFOB was assessed by CXCR4-redistribution

(17) CXCR4-tropic HIV-1 infection in an immunocompetent monkey modelNature CommunicationsMarch 9, 2026Nicholas R. Meyerson, Vanessa L. Bauer, Will T. Fattor, Cody J. Warren, Arturo Barbachano-Guerrero, Melisa J. Weiss, Obaiah Dirasantha, Bridget L. Burris, Adetunji S. Adesina, Joseph L. Timpona, et al.were incubated for 48 h, after which the cells were lysed using the Luciferase Assay System (Promega #E1500) and relative light units (RLUs) were measured using a luminometer. Uninfected cells were used to correct

(18) Myofibroblast lineage mapping and inhibiting subretinal fibrosis by targeting SMAD3 and MRTF pathways via microRNA-24 functional studybioRxivMarch 4, 2026Yinga Wu, Yao Tong, Katherine G. Byrnes, Qi Zhou, Chunmin Dong, Chase Benjamin, Emma Parker, Duran Bao, Zhaoyang Ren, Chastain A. Anderson, et al.internal control. Luciferase activity was measured using the Luciferase Assay System (Promega, Cat. #E1500) according to the manufacturer’s instructions. Adenovirus and infection Adenovirus generation and

(19) An innovative nasal nanovaccine against SARS-CoV-2 induces systemic and upper airway immunity controlling viral replicationnpj VaccinesMarch 4, 2026Roberta L. Pagni, Edecio Cunha-Neto, Yasmin da Silva Santos, Edilberto Postól, Raquel Elaine de Alencar, Ana Moretti, Jonnatan J. Santos, Tamires L. Silva, Isabela P. Daher, Marcos C. Knirsch, et al.HT1080-hACE2 cells. After 48 h, the infected cells were lysed with 5x Luciferase Cell Culture Lysis reagent (#E1500, Promega), and luciferase activity was measured using the Nano-Glo system (#N1110, Promega) in a luminometer

(20) HTLV ‐1 Tax Reshapes the DNA ‐Binding Pattern of Transcription Factor IRF4 and Disrupts Host Gene RegulationCancer ScienceFebruary 25, 2026Shu Tosaka, Jun Mizuike, Motomu Yano, Kaoru Uchimaru, Makoto Yamagishi#E1910), and luciferase activities were measured separately with the Luciferase Assay System (Promega, #E1500) and the Renilla Assay System (Promega, #E2820) according to the manufacturer's instructions. Measurements

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2/9/2016Luciferase Assay System from Promega Haiquan LuWe used this kit for the measurement of luciferase activities after transfection with a luciferase reporter.

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Luciferase Assay System

Luciferase Assay System from Promega

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Promega

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Luciferase Assay System by Promega

Luciferase Assay System From Promega

Luciferase Assay System From Promega

Luciferase Assay System from Promega

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