Luciferase Assay System E1500 from Promega

Product Specs
ItemLuciferase Assay System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE1500
ReactivityMammalian, Bacteria, Plant
SpeciesMammalian,Bacteria,Plant
Detection TargetLuciferase
Sample Typemammalian cells.,plant and bacterial cells,
MethodLuciferase
SensitivityTo 10^{–20
moles of luciferase.}
Assay TimePerform cell lysis, sample preparation and assays in as little as 5 minutes.
Quantity100 assays

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Targeting the αvβ5 Integrin Modifies the TGF-β-Rich Tumor Microenvironment of Pancreatic CancerCancer ResAugust 4, 2026Kodai Suzuki, Yuki Kunisada, Yukihito Kuroda, Hotaka Kawai, Tatiana Hurtado de Mendoza, Evangeline S. Mose, Shingo Eikawa, Henri Havia, Tero A. H. Järvinen, Caisheng Lu, et al.CA). After 6 hours of culture at 37°C, the cells were lysed, mixed with Luciferase Assay Reagent (Cat# E1500; Promega), and analyzed for luminescence intensity using Varioskan LUX (RRID: SCR_026792; Thermo Fisher

(2) Sentinel plants enable quantitative monitoring of bioavailable nitrate in soils and microbial environmentsProc Natl Acad Sci U S AJuly 28, 2026Eugene Li, Chiara Berruto, Tufan M. Oz, Elisa Grillo, Catherine Griffin, Yunqing Wang, Kimberley T. Muchenje, Jolie W. Jones, Gozde S. DemirerPromega; GeNL 520 to 590 nm, NLuc 430 to 485 nm) and the ELuc/RedF pair with the Luciferase Assay System (E1500, Promega; ELuc 490 to 545 nm, RedF 610 to 700 nm); signals were resolved by spectral deconvolution (Promega

(3) MPT0E028, a pan-HDAC inhibitor, ameliorates bleomycin-induced pulmonary fibrosis by promoting AT2-to-AT1 differentiation through the ATM/AMPK/FoxO1 pathwayJ Biomed SciJuly 21, 2026Chia-Hao Liu, Hong-Sheng Lee, Jing-Ping Liou, Wun-Hao Cheng, Hung-Sheng Hua, Bing-Chang Chen, Chien-Huang Lin0.3 μM) for an additional 24 h. Luciferase activity was measured using the Luciferase assay system (E1500, Promega, Madison, WI, USA), and the results were normalized to the expression levels of LacZ. Immunoprecipitation (IP

(4) Activation of EZH2 Promoter Is Mediated by MAPK Signaling Regulated Transcription Factors in Anaplastic Thyroid CancerInt J Mol SciJuly 18, 2026Marcella Maringolo Cristovão, Diego Claro de Mello, Edna Teruko Kimura, Cesar Seigi Fuziwaratransfection, the cell lysate was collected using 1× Passive Lysis Buffer (Promega, Madison, WI, USA, cat. E1500) and luminescence was measured using Dual Luciferase® Reporter Assay kit (Promega) in the luminometer

(5) Synthesis and preliminary evaluation of novel compounds that demonstrate broad host-directed anti-leishmanial activityPLoS Negl Trop DisJuly 13, 2026Elizabeth G. Gurysh, M. Shamim Hasan Zahid, Monica M. Johnson, Antonio Landavazo, Ojas A. Namjoshi, Joseph W. Wilson, Devika M. Varma, Ryan N. Woodring, Aaron T. Hendricksen, Alexandra M. Lopez, et al.of the amastigotes within macrophages was determined using Promega firefly luminescence assay (Cat E1500) or Pierce Renilla luciferase assay (Cat 16166) for L. donovani and L. mexicana strains, respectively.

(6) PCSK5 promotes angiogenesis and cardiac repair after myocardial infarctionNat CommunJuly 3, 2026Jieyu Guo, Siyu Ma, Jinghua Ma, Ming Liu, Fei Ge, Xinhong Wang, Jiayi Lin, Nan Jiang, Xiaoke Lin, Zhipeng Lian, et al.harvested, and luciferase activity was measured with a Luciferase Assay Kit (Promega, Madison, WI, #E1500). β-galactosidase (β-gal). Relative Luc activity was calculated as the ratio of Luc/β-gal activity. Molecular docking

(7) GRHAL1, a novel lncRNA, regulates HIV-1 gene expression by modulating Tat- and Sp1-mediated HIV-1 LTR activationJ Gen VirolJuly 2, 2026Mohammad Ishaq, Heather Marshall, Ven Natarajancell type, and luciferase activity was quantitated using the Luciferase Assay System (Promega, Cat. # E1500). The luciferase activity was normalized to total protein concentration in the cell lysates using the

(8) A subunit vaccine for multiple respiratory virusesSci AdvJune 26, 2026Qinzhe Li, Wei-Chiao Huang, Sara H. Mahmoud, Chengjin Ye, Zachary R. Sia, Yiting Song, Yang Jiao, Yuan Luo, Shiqi Zhou, Reuben M. Pul, et al.incubation, the cells were then washed with PBS and lysed with 30 μl of lysis buffer (Promega, catalog no. E1500) for 10 min at room temperature with gentle shaking. Luciferase substrate (100 μl; CentroPRO catalog

(9) Preclinical evaluation of cysteine protease-inhibitor aloxistatin (E64d) for heart failure therapyJ Mol Med (Berl)June 25, 2026Maria Jordan, Angelika Stucki-Koch, Maximilian Schinke, Lena Willmer, Ingrid Senne, Naisam Abbas, Marco Bentele, Dana Oldenkott, Nico Lachmann, Linda Feldbrügge, et al.4 °C and 300 × g. Supernatant was removed, pellets were lysed in 70 µL Cell Culture Lysis Reagent (E1500, Luciferase Assay System, Promega, Walldorf, Germany) on ice for 15 min and cleared by centrifuge at

(10) MAP3K1 integrates RhoA/ROCK signaling to regulate epithelial morphogenesisiScienceJune 19, 2026Bo Xiao, Maureen Mongan, Young-Bum Kim, Ying Xiawere lysed, and luciferase activity was measured using the Luciferase Assay System (Promega, Cat. #E1500; Madison, WI, USA) following the manufacturer’s instructions. Luminescence was determined by a SpectraMax M5

(11) Non-viral targeted integration at the CISH locus enables CAR-NK cell engineering with enhanced anti-tumor activityMolecular Therapy OncologyJune 18, 2026Jiao Wang, Yao Sun, Jakob Starzyk, Fei Wang, Xin Dong, Richard Shan, Xuemei He, Keqiang Xie, Guozhu Xie, Hao Wuco-culture, relative luciferase activities were measured using the Promega Luciferase Assay System (Cat# E1500) according to the manufacturer’s instructions. Specific lysis of each group was calculated using the

(12) B7 homolog 3-targeted CAR-T cells secreting EGFR T-cell engagers for improved control of glioblastoma progressionMol BiomedJune 16, 2026Zongliang Zhang, Nian Yang, Hui Zeng, Yongdong Chen, Huaqing Lu, Long Xu, Zeng Wang, Guoqing Wang, Liangxue Zhou, Aiping Tongwere combined with 100 μL of CAR‑T supernatant. At the end of the incubation, a luciferase assay kit (#E1500, Promega) was used to lyse the cells, and luminescence was quantified on a BioTek Synergy H4 hybrid

(13) KLF6 activation marks an angiogenic and apoptosis resistant endothelial phenotype in pulmonary arterial hypertensionCommun BiolJune 15, 2026Rehab Alharbi, Merve Keles, Nadia Fernandes, Hannah Maude, Adam Fellows, Richard D. Williams, Chien-Nien Chen, Nathalie Lambie, Nik Matthews, May Al-Sahaf, et al.either normoxic or hypoxic conditions for 24 h. A luciferase reporter assay (Promega, USA, Cat. No. E1500) was then performed according to the manufacturer’s instructions. The intensity of luminescence, proportional

(14) SnRK2.6-mediated phosphorylation stabilizes proteasome regulator 1 (PTRE1) to enhance 26S proteasome activity and coordinate ABA signaling in ArabidopsisPlant CommunicationsJune 8, 2026Peng-Chao Hao, Ling-Li Zheng, Jin-Shuang Dou, Hong-Wei Xueharvested for lysis. LUC activity was measured using 20 μL of lysate and 100 μL of LUC assay reagent (E1500, Promega) with a luminometer (Varioskan Flash microplate reader, Thermo Scientific). GUS activity was

(15) Human angiotensin‑converting enzyme 2‑specific benzothiazole-based allosteric inhibitor against pan‑sarbecovirusesNature CommunicationsJune 6, 2026Li Liu, Jinxin Bai, Runhong Zhou, Yao Ma, Dongyan Zhou, Terrence Tsz-Tai Yuen, Wenwu Liu, Pok Man Lai, Wenjun Yu, Jianxin Duan, et al.cells. After 48–72 h, infected cells were lysed to measure luciferase activity using a commercial kit (E1500, Promega, Madison, WI). Luciferase activity was measured using a Varioskan LUX Multimode Microplate reader

(16) Polyfunctional antibody signature defines protection in Andes hantavirus survivalFront ImmunolJune 5, 2026Felipe Bravo, Tybbysay P. Salinas, Camila Kossack, Jorge T. Gonzalez, Israel Giacaman, Claudio Quevedo, Sebastián Fuller, Farides Saavedra, Claudio Aguilera-Pérez, Felipe Aguilera, et al.(Promega E1531), IgG-dependent activation was quantified by measuring luciferase activity (Promega E1500) on a TECAN M200PRO plate reader, with background signals (from non-opsonized controls) subtracted to

(17) PTBP1 is upregulated in response to Zika virus infection and restrains viral replication by hijacking viral NS1 protein to induce NS1 degradationJournal of VirologyMay 19, 2026Menglan Rao, Yue Kong, Shuang Liu, Zicong Chen, Jiuxiu Lin, Yicong Liang, Zhiwei Lei, Zhen LuoLuciferase reporter assay Luciferase reporter assay was processed by using Firefly Luciferase Reporter Assay System (Cat #E1500) (Promega) according to the manufacturer’s instructions. A549 cells were seeded in 24-well plates and

(18) Dual site targeting of the bacterial 70S ribosome by tetracyclinesNat CommunMay 19, 2026Swapnil C. Devarkar, Ivan B. Lomakin, Jimin Wang, Ayman Grada, Christopher G. Bunickwas detected in 1–2 min after addition of 50 μl of the Luciferase Assay Reagent (Promega, USA, cat. # E1500) using multimode plate reader TriStar LB941 (Berthold technologies LTD, UK) and analyzed using Prism

(19) Stem cell activity is linked to a transient acquisition of pluripotency during callus proliferationNature CommunicationsMay 19, 2026Soon Hyung Bae, Ok-Sun Park, Kyounghee Lee, Hong Gil Lee, Seo Young Jang, Geum-Sook Hwang, Yoo-Sun Noh, Pil Joon Seoas an internal control. The LUC assay was performed using the Luciferase Assay System Kit (Promega, E1500). ChIP-qPCR Protoplasts were isolated and transfected using the same method described in the transient

(20) Nuclear factor IX promotes endometriosis progression through transcriptional activation of tetraspanin-2J Mol Med (Berl)May 16, 2026Yuqi Liu, Qing Xue, Jingwen Zhu, Cheng Zeng, Xin Li, Jing Shang, Jie Li, Peili Wuinternal control. Cells were gathered 48 h post transfection, and the luciferase assay system (Promega, E1500) was used to measure the activity of luciferase. The activity of firefly luciferase was standardized

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2/9/2016Luciferase Assay System from Promega Haiquan LuWe used this kit for the measurement of luciferase activities after transfection with a luciferase reporter.

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Luciferase Assay System

Luciferase Assay System from Promega

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Promega

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Luciferase Assay System by Promega

Luciferase Assay System From Promega

Luciferase Assay System From Promega

Luciferase Assay System from Promega

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