Luciferase Assay System E1500 from Promega

Product Specs
ItemLuciferase Assay System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE1500
ReactivityMammalian, Bacteria, Plant
SpeciesMammalian,Bacteria,Plant
Detection TargetLuciferase
Sample Typemammalian cells.,plant and bacterial cells,
MethodLuciferase
SensitivityTo 10^{–20
moles of luciferase.}
Assay TimePerform cell lysis, sample preparation and assays in as little as 5 minutes.
Quantity100 assays

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) A subunit vaccine for multiple respiratory virusesSci AdvJune 26, 2026Qinzhe Li, Wei-Chiao Huang, Sara H. Mahmoud, Chengjin Ye, Zachary R. Sia, Yiting Song, Yang Jiao, Yuan Luo, Shiqi Zhou, Reuben M. Pul, et al.incubation, the cells were then washed with PBS and lysed with 30 μl of lysis buffer (Promega, catalog no. E1500) for 10 min at room temperature with gentle shaking. Luciferase substrate (100 μl; CentroPRO catalog

(2) Preclinical evaluation of cysteine protease-inhibitor aloxistatin (E64d) for heart failure therapyJ Mol Med (Berl)June 25, 2026Maria Jordan, Angelika Stucki-Koch, Maximilian Schinke, Lena Willmer, Ingrid Senne, Naisam Abbas, Marco Bentele, Dana Oldenkott, Nico Lachmann, Linda Feldbrügge, et al.4 °C and 300 × g. Supernatant was removed, pellets were lysed in 70 µL Cell Culture Lysis Reagent (E1500, Luciferase Assay System, Promega, Walldorf, Germany) on ice for 15 min and cleared by centrifuge at

(3) MAP3K1 integrates RhoA/ROCK signaling to regulate epithelial morphogenesisiScienceJune 19, 2026Bo Xiao, Maureen Mongan, Young-Bum Kim, Ying Xiawere lysed, and luciferase activity was measured using the Luciferase Assay System (Promega, Cat. #E1500; Madison, WI, USA) following the manufacturer’s instructions. Luminescence was determined by a SpectraMax M5

(4) Non-viral targeted integration at the CISH locus enables CAR-NK cell engineering with enhanced anti-tumor activityMolecular Therapy OncologyJune 18, 2026Jiao Wang, Yao Sun, Jakob Starzyk, Fei Wang, Xin Dong, Richard Shan, Xuemei He, Keqiang Xie, Guozhu Xie, Hao Wuco-culture, relative luciferase activities were measured using the Promega Luciferase Assay System (Cat# E1500) according to the manufacturer’s instructions. Specific lysis of each group was calculated using the

(5) B7 homolog 3-targeted CAR-T cells secreting EGFR T-cell engagers for improved control of glioblastoma progressionMol BiomedJune 16, 2026Zongliang Zhang, Nian Yang, Hui Zeng, Yongdong Chen, Huaqing Lu, Long Xu, Zeng Wang, Guoqing Wang, Liangxue Zhou, Aiping Tongwere combined with 100 μL of CAR‑T supernatant. At the end of the incubation, a luciferase assay kit (#E1500, Promega) was used to lyse the cells, and luminescence was quantified on a BioTek Synergy H4 hybrid

(6) KLF6 activation marks an angiogenic and apoptosis resistant endothelial phenotype in pulmonary arterial hypertensionCommun BiolJune 15, 2026Rehab Alharbi, Merve Keles, Nadia Fernandes, Hannah Maude, Adam Fellows, Richard D. Williams, Chien-Nien Chen, Nathalie Lambie, Nik Matthews, May Al-Sahaf, et al.either normoxic or hypoxic conditions for 24 h. A luciferase reporter assay (Promega, USA, Cat. No. E1500) was then performed according to the manufacturer’s instructions. The intensity of luminescence, proportional

(7) SnRK2.6-mediated phosphorylation stabilizes proteasome regulator 1 (PTRE1) to enhance 26S proteasome activity and coordinate ABA signaling in ArabidopsisPlant CommunicationsJune 8, 2026Peng-Chao Hao, Ling-Li Zheng, Jin-Shuang Dou, Hong-Wei Xueharvested for lysis. LUC activity was measured using 20 μL of lysate and 100 μL of LUC assay reagent (E1500, Promega) with a luminometer (Varioskan Flash microplate reader, Thermo Scientific). GUS activity was

(8) Polyfunctional antibody signature defines protection in Andes hantavirus survivalFront ImmunolJune 5, 2026Felipe Bravo, Tybbysay P. Salinas, Camila Kossack, Jorge T. Gonzalez, Israel Giacaman, Claudio Quevedo, Sebastián Fuller, Farides Saavedra, Claudio Aguilera-Pérez, Felipe Aguilera, et al.(Promega E1531), IgG-dependent activation was quantified by measuring luciferase activity (Promega E1500) on a TECAN M200PRO plate reader, with background signals (from non-opsonized controls) subtracted to

(9) Dual site targeting of the bacterial 70S ribosome by tetracyclinesNat CommunMay 19, 2026Swapnil C. Devarkar, Ivan B. Lomakin, Jimin Wang, Ayman Grada, Christopher G. Bunickwas detected in 1–2 min after addition of 50 μl of the Luciferase Assay Reagent (Promega, USA, cat. # E1500) using multimode plate reader TriStar LB941 (Berthold technologies LTD, UK) and analyzed using Prism

(10) PTBP1 is upregulated in response to Zika virus infection and restrains viral replication by hijacking viral NS1 protein to induce NS1 degradationJournal of VirologyMay 19, 2026Menglan Rao, Yue Kong, Shuang Liu, Zicong Chen, Jiuxiu Lin, Yicong Liang, Zhiwei Lei, Zhen LuoLuciferase reporter assay Luciferase reporter assay was processed by using Firefly Luciferase Reporter Assay System (Cat #E1500) (Promega) according to the manufacturer’s instructions. A549 cells were seeded in 24-well plates and

(11) Nuclear factor IX promotes endometriosis progression through transcriptional activation of tetraspanin-2J Mol Med (Berl)May 16, 2026Yuqi Liu, Qing Xue, Jingwen Zhu, Cheng Zeng, Xin Li, Jing Shang, Jie Li, Peili Wuinternal control. Cells were gathered 48 h post transfection, and the luciferase assay system (Promega, E1500) was used to measure the activity of luciferase. The activity of firefly luciferase was standardized

(12) Poly-GR promotes ferroptosis-associated vulnerability in C9orf72-ALSCell & BioscienceApril 26, 2026Chun-Yu Lin, Wen-Chi Hsieh, Shao‐Ming WangPolyJet reagent (SignaGen Laboratories). Cell extracts were prepared using the Luciferase Assay System (E1500; Promega) following the manufacturer’s protocol, and luminescence was measured using a SpectraMax iD3

(13) Mitochondrial AK3 inhibits nuclear β-catenin localization and its activation through enhancing mitochondrial activityCell Death & DiseaseApril 22, 2026Muhah Jeong, Shin-Hyeon Ryu, Young-Sin Cho, Do-Hyeong Na, Jongyeon Baek, Jihoon Nah, Ki Woo Kim, Yong-Keun Jungcells were detached using trypsin-EDTA, lysed, and analyzed using the Luciferase Assay System (Promega, E1500) according to the manufacturer’s instructions. Recombinant human Wnt3a protein (Proteintech, HZ-1296)

(14) Multimodal profiling reveals cell type-specific pseudouridine modification and density-dependent translational regulationNucleic Acids ResApril 21, 2026Caroline A McCormick, Michele Meseonznik, Yuchen Qiu, Oleksandra Fanari, Priyanka Goyal, Mitchell Thomas, Mina Shokoufandeh, Yifang Liu, Dylan Bloch, Cole Greenfield, et al.placed on ice to stop translation. To measure protein abundance, the Luciferase Assay System (Promega, E1500) was used. 2.5 μl of each Fluc RRL sample was added to separate wells. Fifty microliters of the luciferase

(15) Targeting DNA Polymerase Epsilon Induces Tumor Clearance and Activates an NF-κB-Mediated Inflammatory Response in Triple Negative Breast CancerCancer ResApril 21, 2026Elizabeth F. Sher, Kenji M. Fujihara, Anthony Tao, Paul Sastourne-Haletou, Diana Erenburg, Vladislav O. Sviderskiy, Hannan Mir, Triantafyllia Karakousi, Cynthia A. Loomis, Jiehui Deng, et al.Polyethylenimine (Polysciences, Cat# 239662) was from Polysciences. Luciferase Assay System kit (Promega, Cat# E1500) was from Promega. Lentiviral shRNAs were obtained from the The RNAi Consortium (TRC) collection of

(16) VAP1 promotes cardiac fibrosis by enabling PDGFR signaling in myofibroblastsExperimental & Molecular MedicineApril 20, 2026Shan Huang, Qianwen Zhao, Tinghui Shao, Chenghao Zhu, Yujia Xue, Naxia Chen, Yue Zhang, Huihui Xu, Ming Kong, Rui Wangtransfection. Reporter assay was performed with a luciferase assay system, as per vendor's instructions (E1500, Promega). Protein extraction, immunoprecipitation, and western blotting Whole-cell/tissue lysates

(17) Inhalable Perfluorocarbon RNA Nanocapsules Bypass Immune Clearance While Targeting Lung Epithelial and Lung Tumor CellsbioRxivApril 12, 2026Kasturi Siddhanta, Atefehsadat Monirvaghefi, Aditya Sundar, Braeden R. Pinkerton, Neha Kumari, Ling Ding, C. J. Woslager, Marjina Akter Kalpana, Chinmay M. Jogdeo, Ashley R. Ravnholdt, et al.buffer, and luciferase activity was quantified using the Promega Luciferase Assay System (Cat. No. E1500). CXCR4 inhibition assay: CXCR4 inhibitory activity by PAMD-C@PFOB was assessed by CXCR4-redistribution

(18) Genetic and pharmaceutical manipulation of H3K9 methyltransferase Suv39h1 promotes liver regeneration by unleashing HMGB2 transcriptionExperimental & Molecular MedicineApril 10, 2026Yunjie Lu, Jiawen Zhou, Xiulian Miao, Sheng Zeng, Lei Qin, Yong Xu, Shuai Wang, Zilong Liactivities were assayed at 24–48 h after transfection using a luciferase reporter assay system (Promega, E1500) as previously described28. Recombinant murine HGF (R&D, 2207-HG) was reconstituted in sterile PBS and

(19) The COX2-PGE2-PKA Axis Suppresses Antiviral Immunity by Inhibiting mtDNA-Dependent STING ActivationbioRxivApril 6, 2026Pham Thuy Tien Vo, Julien Cicero, Zichen Wang, Hiroyuki Hakozaki, Thomas S Hoang, Sendi Rafael Adame-Garcia, Dana J. Ramms, Kuniaki Sato, Erica Stevenson, Yuan Zhou, et al.figure legends. Luciferase assays were performed with the Firefly Luciferase Assay System (Promega, E1500) according to the manufacturer’s instructions. RNA extraction, cDNA synthesis and quantitative real-time PCR

(20) Sodium- myo -inositol cotransporter-1, SMIT1, promotes cardiac hypertrophy and fibrosis induced by pressure overload in miceCardiovascular ResearchMarch 26, 2026Alice Marino, Julien Cumps, Laura Guilbert, Angéline Geiser, Claire Baufays, Audrey Ginion, Laura Ferté, Sylvain Battault, Fiona De Matos, Jerome Ambroise, et al.NFAT-luciferase assay Luciferase activity was measured in supernatants using the Luciferase Assay System (LAR, E1500, Promega) following the manufacturer’s instruction. Briefly, 20 µL of cell lysate (800 000 cells/well

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2/9/2016Luciferase Assay System from Promega Haiquan LuWe used this kit for the measurement of luciferase activities after transfection with a luciferase reporter.

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Luciferase Assay System

Luciferase Assay System from Promega

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Promega

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Luciferase Assay System by Promega

Luciferase Assay System From Promega

Luciferase Assay System From Promega

Luciferase Assay System from Promega

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