Bright-Glo Luciferase Assay System E2610 from Promega

Product Specs
ItemBright-Glo Luciferase Assay System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE2610
ReactivityMammalian
Detection TargetLuciferase
SpeciesMammalian
Sample TypeMammalian cells
MethodLuciferase
Quantity10ml
SensitivityUp to tenfold more light intensity than other homogeneous luciferase assay reagents results in increased sensitivity.

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Sialic acid cis-ligand dynamics modulate Siglec-7 and -9 function and affect Siglec-7/9 co-blockade to potentiate natural killer cell anti-tumor activityOncoimmunologyDecember 31, 2026Jesper van Eck van der Sluijs, Anne H. C. Valk, Eline J. H. van Houtum, Esther D. Kers-Rebel, Maaike Looman, Willemijn Hobo, Anna E. Marneth, Harry Dolstra, Lune van den Boogaard, Christian Büll, et al.and 5% CO2, followed by luminescence measurement using Bright-Glo™ Luciferase Assay System (Promega, E2620) according to the manufacturer’s protocol on a Victor3 Multilabel Plate Reader (Perkin Elmer). Western blot

(2) DNA-based delivery of incretin receptor agonists using MYO Technology leads to durable weight loss in a diet-induced obesity modelMol Ther Nucleic AcidsJune 16, 2026Linda Sasset, Andrew D. Cameron, Carleigh Sussman, Luisa Rubinelli, Debnath Maji, Robert Miller, Andy Thompson, Delcora Campbell, Melanie R. Walker, Marek M. Drozdz, et al.IRAs. After 3 h of incubation, cells were lysed with Bright-Glo Luciferase Assay System (Promega, cat# E2610) according to the manufacturer’s protocol, and the luminescence was measured using GloMax Navigator

(3) Identification of a unique humoral immune signature of Sudan ebolavirus persistence in human survivorsResearch SquareMay 27, 2026Shuangyi Gunn, Shuangyi Muwonge, Carolyne Nasimiyu, Carolyne Bakamutumaho, Barnabas Bakamutumaho, Cynthia Ulusan, Peter James Ulusan, Moses L. Elyanu, Robert F. Breiman, Robert Ssewanyana, et al.activity was measured by adding 30 μl per well of Bright-Glo luciferase substrate (Promega, cat. #. E2620) and reading luminescence on a Tecan Spark luminometer (Life Sciences). Percent neutralization was calculated

(4) A targetable opioid/cancer associated fibroblast axis drives extracellular matrix remodeling and tumor aggressiveness in pancreatic cancerbioRxivMay 26, 2026Kathryn E. Maraszek, Arwen A. Tisdale, Hunter D. Reavis, Xiaozhuo Liu, Eduardo Cortes Gomez, Aleksandr Dolskii, Caneta Brown, Daksh Thakkar, Maura Dungan, Anusha Adhikari, et al.the respective conditions. On day 5, the Promega Bright-Glo Luciferase Assay System (Promega, cat. #E2610) was used to measure the luminescence of each well according to the manufacturer’s protocol. Extra wells

(5) Protease-activated receptor 1 as an endogenous model of peptidergic Gαq-Gα12-biased G protein signalingFrontiers in Molecular BiosciencesMay 25, 2026Braden S. Fallon, Robert A. Campbell, Justin G. Englisha PHERAstar FSX (BMG Labtech, RRID:SCR_027001) using the Bright-Glo Luciferase Assay (Promega, Cat# E2610). Bright-Glo reagent was diluted in an equal volume of assay buffer (20 mM HEPES in HBSS), and 10 μL

(6) TMPRSS2-induced Golgi disruption restricts the incorporation of virus envelope glycoproteins into virionsEMBO ReportsMay 19, 2026Sayuri Seki, Shigeyoshi Harada, Akiko Sugimoto-Ishige, Midori Unno, Machie Sakuma, Shinsuke Ito, Hiroyuki Yamamoto, Aki Tanabe, Saori Matsuoka, Chieko Makino-Okamura, et al.kit,ZeptoMetrix,0801008 Glo lysis buffer,Promega,E2661 Bright-Glo luciferase assay system,Promega,E2620 carboxymethyl cellulose sodium salt,FujiFilm Wako,039-01335 neutral buffered formalin,Muto Chemical,20214 crystal

(7) Algorithm-Optimized H5 Influenza mRNA Vaccine Induces Broad Immune ResponsesInt J Mol SciMay 19, 2026Liangliang Wang, Zhengda Peng, Chenchen He, Jie Zhang, Pengju Yu, Weijin Huang, Youchun Wang, Chenyan Zhaoh. After removing the supernatant, luciferase activity was measured using a luciferase assay system (E2620, Promega) with 2 min of shaking. Luminescence was quantified using a PerkinElmer EnSight(Waltham, MA,

(8) Genomic and phenotypic comparisons reveal lineage-specific traits of the probiotic Saccharomyces boulardiiiScienceMay 15, 2026Hannah Elena Duffey, Karl Alex Hedin, Hitesh P. Gelli, Troels Holger Vaaben, Morten Otto Alexander Sommerassays ,, QUANTI-Luc reagent,InvivoGen,Cat. No. rep-qlc4r2 Bright-Glo Luciferase assay,Promega,Cat. No. E2610 IL-8 ELISA kit,R&D Systems,Cat. No. DY208 TNFα ELISA kit,Abcam,Cat. No. ab181421 Yeast Live-or-Dye™

(9) Distinct cytokines regulate gene expression and anti-tumor activity in regenerated CD8+ T cells from induced pluripotent stem cellsiScienceMay 15, 2026Kenta Kondo, Nobuhito Nitta, Koji Terada, Yusuke Sano, Mina Kumode, Seiji Nagano, Kyoko Masuda, Daisuke Okuzaki, Toru Miyake, Masaji Tani, et al.hours. The luciferase activity was measured using the Bright-Glo™ Luciferase Assay System (Promega, E2610) and expressed as relative light units (RLU). Target cells alone were cultured alone to measure the

(10) De novo and scaffold-based design of GDF15 binders for cancer cachexia diagnostics and therapeuticsExperimental & Molecular MedicineMay 8, 2026Jinsook Ahn, Ryeongeun Cho, Sohyun Kim, Dong Sun Lee, Ho Min KimGlo-lysis buffer (#E2661, Promega) for 10 min, followed by the addition of Bright-Glo luciferase reagent (#E2620, Promega) in a white opaque 96-well plate (#3917, Corning). After incubation for 5 min, luminescence

(11) Protocol for lentiviral generation and transduction of human-derived endometrial epithelial organoids to study uterine function and pathologiesSTAR ProtocMay 7, 2026Sydney E. Parks, Diana Monsivaisassays,Critical commercial assays,Critical commercial assays ,, Bright-Glo Luciferase Assay System,Promega,Cat# E2610 Direct-zol RNA Miniprep,Genesee,Cat# 11-331 ,, Experimental models: Cell lines,Experimental models:

(12) Multifunctional ACE2-nanobody fusion design for pan-specific neutralization and cardiovascular protection in SARS coronavirus infectionAntimicrobial Agents and ChemotherapyMay 6, 2026Traian Sulea, Matthew Stuible, Maria Moreno, Alex Pelletier, Martin A. Rossotti, Yuneivy Cepero-Donates, Jacqueline Slinn, Patrick Salois, Anh Tran, Melissa Hewitt, et al.was then measured by luminescence. Bright-Glo luciferase reagent (Promega, Madison, WI, USA, Cat no. E2620) was added to wells for 2 min before reading with a PerkinElmer Envision instrument. Half-maximal inhibitory

(13) Intranasal Replicating Adenovirus type 4-Vectored SARS-CoV-2 Vaccines Induce Durable and Efficacious Responses in Preclinical TestingiScienceApril 17, 2026Breanna Kim, Rachel Roenicke, Peyton M. Roeder, Isabel Steinberg, Andy Patamawenu, Mitra Harrison, Freya van’t Veer, Emma Koory, Jane Xie, Tulley Shofner, et al.Ltd,Cat#067020 AdEasy Viral Titer Kit,Agilent Technologies,Cat#972500 Bright Glo Luciferase Assay System,Promega,Cat#E2610 Moderna mRNA −1273 vaccine,Moderna US Inc,Cat#80777011096 Luminex MagPlex Magnetic Microspheres,Diasorin,https://us.diasorin.com/en/luminex/products-and-services/microspheres/magplex-microspheres ZR BAC DNA Miniprep Kit

(14) Thymelaea hirsuta (L.) Endl. extract attenuates NLRP3 inflammasome activation via modulation of ATPase activityFront PharmacolApril 15, 2026Seongjong Lee, Seoyeon Jang, Hangyeol Lee, Ali Khorchani, Abdelhamid Khaldi, Mi Jin Lee, Dong Keun Yi, Soo-Yong Kim, Sangho Choi, Hyungseok Seo, et al.DAPI (ab104139; Abcam, United Kingdom). For reporter assays, the Bright-Glo™ Luciferase Assay System (E2610), pGL4.32 [luc2P/NF-κB-RE/Hygro] vector (E8491), and the ADP-Glo™ Max Assay (V7001) were purchased from

(15) ACE2 utilization of HKU25 clade MERS-related coronaviruses with broad geographic distributionbioRxivApril 12, 2026Chen Liu, Young-Jun Park, Cheng-Bao Ma, Cameron Stuart, Risako Gen, Yu-Cheng Sun, Xiao Yang, Mei-Yi Lin, Qing Xiong, Jun-Yu Si, et al.(Relative light units, RLU) was measured at 18 hpi using the Bright-Glo Luciferase Assay Kit (Promega, E2620) and detected with a GloMax 20/20 Luminometer (Promega) or Varioskan LUX Multi-well Luminometer (Thermo

(16) NF-κB-Driven HIV-1 Gene Expression in Human Cells Is Independent of Poly(ADP-ribose) polymerase-1 FunctionbioRxivApril 11, 2026Denisse A. Gutierrez, Manuel Llanoanalyzed for luciferase activity with a luminescence kit (Bright-Glo™ Luciferase Assay System, Promega, E2620) as described in (64). In some experiments, ATP was measured in the same samples using the CellTiter-Glo® Luminescent Cell Viability Assay

(17) Intragenic Transcription from Defective HIV Proviruses Triggers Interferon Responses in Myeloid CellsbioRxivApril 7, 2026Jonathan Kilroy, Aparna Deokar, Samantha Patalano, Juan Fuxman Bass, Manish Sagar, Suryaram Gummuluru, Andrew J. HendersonMedia/supernatant was aspirated from these plates and 30 μL of BrightGlo reagent (Promega Catalog No. E2610) were added to lyse the cells and measure luciferase activity. A standard curve was generated by adding

(18) Sialic acid cis-ligand dynamics modulate Siglec-7 and -9 function and affect Siglec-7/9 co-blockade to potentiate natural killer cell anti-tumor activityOncoimmunologyApril 2, 2026Jesper van Eck van der Sluijs, Anne H. C. Valk, Eline J. H. van Houtum, Esther D. Kers-Rebel, Maaike Looman, Willemijn Hobo, Anna E. Marneth, Harry Dolstra, Lune van den Boogaard, Christian Büll, et al.and 5% CO2, followed by luminescence measurement using Bright-Glo™ Luciferase Assay System (Promega, E2620) according to the manufacturer’s protocol on a Victor3 Multilabel Plate Reader (Perkin Elmer). Western blot

(19) TNF inhibits SARS-CoV-2 induced cell-cell fusion through activating the SDC4-RhoA signaling to promote actin bundles formationCell InsightApril 1, 2026Dong Duan, Xu Zheng, Yanqiu Zhou, Mengmeng Cui, Yunyi Li, Xiaoxian Cui, Yuying Yang, Min Chen, Huanyu Wu, Xin Chen, et al.EDTA-free protease inhibitor cocktail (Roche)). Lysates were mixed with Bright-Glo luciferase substrate (E2610, Promega), and relative luminescence units (RLU, 1-min read) were measured using a Synergy H1 plate

(20) Receptor-guided AAV Tropism Engineering via MATCHbioRxivApril 1, 2026Nolan Graham, Satheesh Kumar, Joseph Rainaldi, Seoin Yang, Andrew Portell, Billy Santoso, Prashant MaliCK04-01) before luciferase activity was assessed using the Bright-Glo™ Luciferase Assay System (Promega, E2610) according to manufacturer’s protocols. Luciferase signal was then normalized to relative cell viability. Human

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Bright-Glo Luciferase Assay System

Bright-Glo Luciferase Assay System from Promega

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Promega

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Bright-Glo™ Luciferase Assay From Promega

Bright-Glo™ Luciferase Assay System From Promega

Bright-Glo Luciferase Assay System from Promega

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