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Bright-Glo Luciferase Assay System
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Bright-Glo Luciferase Assay System
Bright-Glo Luciferase Assay System
Bright-Glo Luciferase Assay System from Promega
Be the first to write a review!
Citations:
(596)
Figures:
(4)
Supplier Page
Supplier Page from
Promega for
Bright-Glo Luciferase Assay System
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Description
Bright-Glo Luciferase Assay System is designed for continuous-process systems. The robust, homogeneous assay chemistry gives high assay sensitivity and has a 30-minute signal half life
Product Specs
Company Info
Citations
Reviews
Product Reviews
Product Specs
Item
Bright-Glo Luciferase Assay System
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
E2610
Reactivity
Mammalian
Detection Target
Luciferase
Species
Mammalian
Sample Type
Mammalian cells
Method
Luciferase
Quantity
10ml
Sensitivity
Up to tenfold more light intensity than other homogeneous luciferase assay reagents results in increased sensitivity.
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Promega
2800 Woods Hollow Rd
Madison
,
WI
53711
United States
Phone
: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516
Email: custserv@promega.com
techserv@promega.com
Company Profile
Website:
http://www.promega.com
Citations (596)
(1)
Sialic acid cis-ligand dynamics modulate Siglec-7 and -9 function and affect Siglec-7/9 co-blockade to potentiate natural killer cell anti-tumor activity
Oncoimmunology
December 31, 2026
Jesper van Eck van der Sluijs, Anne H. C. Valk, Eline J. H. van Houtum, Esther D. Kers-Rebel, Maaike Looman, Willemijn Hobo, Anna E. Marneth, Harry Dolstra, Lune van den Boogaard, Christian Büll,
et al.
and 5% CO2, followed by
luminescence measurement
using
Bright-Glo™ Luciferase Assay System
(
Promega
,
E2620
) according to the manufacturer’s protocol on a Victor3 Multilabel Plate Reader (Perkin Elmer).
Western blot
(2)
Emergence of neutralizing RBD antibodies following Omicron infection with limited activity against ancestral SARS-CoV-2
iScience
August 21, 2026
Michael S. Piepenbrink, Yao Ma, Simran Panjwani, Alexandra R. Blake, Allison M. Bell, James L. Kizziah, Sara H. Mahmoud, Gregory C. Ippolito, Nathan B. Erdmann, Paul A. Goepfert,
et al.
(Fc) capture kit,Cytival Life Sciences,Cat#BR100839
Bright-Glo™ Luciferase Assay System
,
Promega
,Cat#:
E2620
,, Deposited data,Deposited data,Deposited data ,, 1332D4-S(KP3.1.1) Structure,This study,PDBID
(3)
SARS-CoV-2 Neutralization Breadth Is Shaped by Variant Exposure and Immune History
J Med Virol
August 13, 2026
Greta Vizgirda, Alexander P. Underwood, Ulrik Fahnøe, Christina Sølund, Signe Lysemose Villadsen, Andrea Galli, Nina Weis, Jens Bukh
quantified by measuring
luminescence
using the
Bright‐Glo Luciferase Assay System
(
Promega
; Cat. No.
E2610
) and the
FLUOstar Omega
microplate reader (
BMG Labtech
).
Infection
was considered positive when
luminescence
(4)
Directed evolution of a stem-helix-targeting antibody enables MERS-CoV cross-neutralization through enhanced binding affinity
PLoS Pathog
August 7, 2026
Panpan Zhou, Meng Yuan, Yuexiu Zhang, Oliver Limbo, Ge Song, Fangzhu Zhao, Hejun Liu, Wan-Ting He, Tazio Capozzola, Sean Callaghan,
et al.
well. After 48h of
incubation
, luciferase activity was measured by BrightGlo substrate (
Promega
Cat#
E2620
) according to the manufacturer’s instructions. Fifty percent maximal inhibitory concentrations (IC50s)
(5)
Terminus-specific antibody development and bioanalytical assay validation to quantify active DR10624 in pharmacokinetic studies
Front Pharmacol
August 3, 2026
Zhenxing Zhou, Kexin Cao, Gaofeng Yao, Yuanyuan Liu, Wenwen Duan, Chengcheng Yang, Shimei Sheng, Yanshan Huang
incubation
, the cells were lysed using the
Promega
Bright-Glo™ Luciferase Assay System
(catalog number:
E2620
; Madison, WI, USA) and luciferase activity was measured using the Molecular Devices I3 microplate reader
(6)
Engineering viral protease-operated nanobodies for programmable and orthogonal control of protein function
Nature Communications
July 30, 2026
Mingguang Cui, Xiaoxuan Liu, Tien-Hung Lan, Tianlu Wang, Tatsuki Nonomura, Brendan McKee, Rui Wang, Yun Huang, Yubin Zhou
HY-106161
) were purchased from
MedChemExpress
. For luciferase
assays
,
Bright-Glo Luciferase Assay System
(
E2610
) was purchased from
Promega
. For SEAP
assay
, Diethanolamine Substrate Buffer (5X) (
34064
) and PNPP tablets
(7)
Identification of Entacapone as a Novel β-Arrestin 1 Biased Antagonist of CXCR7
Molecules
July 26, 2026
Liangrui Shi, Yan Huang, Lian Li, Huan Li, Xin Li, Zenghao Bi, Junke Liu, Sanyin Zhang, Zhaotong Cong, Bojun Wang,
et al.
room-temperature PBS. According to the manufacturer’s instructions, Bright-Glo™ luciferase
assay
reagent (
Promega
,
E2610
) was diluted with 1 × PBS, and 50 μL was added to each well. The plate was incubated in a
cell culture
(8)
Strain-specific epistasis shapes fitness landscapes of APOBEC3G antagonism by HIV-1 Vif proteins
Sci Adv
July 24, 2026
Caroline A. Langley, Michelle Lilly, Harmit S. Malik, Michael Emerman
DEAE-dextran (20 μg/ml). At 72 hours postinfection, cells were lysed in Bright-Glo reagent (
Promega
, #
E2610
), and luciferase activity was measured on a
LUMIstar Omega
luminometer (
BMG Labtech
). Each condition
(9)
Prioritizing neuroactive ligands using motif-guided virtual discovery and zebrafish profiling
NPJ Drug Discov
July 21, 2026
Ari B. Ginsparg, Jaqueline A. Martinez, Ishaan Patel, Angélique Buton, Laura T. Lee, Raheel Sarwar, Rocco Moretti, Stephanie Puig, Summer B. Thyme
After
incubation
for 24 h at 37 °C, wells were treated with 5 μL of BrightGlo reagent (
Promega
- cat#
E2610
) and incubated in darkness at room temperature for 15 min. A back seal sticker (Perkin Elmer) was applied
(10)
Genetic diversity of pangolin coronaviruses reveals a key immuno-evasive substitution at spike residue 519
Journal of Virology
July 21, 2026
Maximilian Stanley Yo, Yu Kaku, Yusuke Kosugi, Jarel Elgin Tolentino, Daisuke Kuroda, Yunlong Cao, Kei Sato
post-
infection
, the infected cells were lysed with a BrightGlo luciferase
assay
system (
Promega
, Cat#
E2620
), and the luminescent signal produced by
firefly
luciferase reaction was measured using a GloMax Explorer
(11)
Novel 3-Amino-2-methylquinazolinone NF-κB Inhibitors: Synthesis and Potential Anti-Inflammatory Function
Int J Mol Sci
July 20, 2026
Chrysoula Mikra, Vasiliki Petriki, Evangelos Tsioupros, Eleni Kougioumtzian, Konstantinos Michail, Stella Manta, Barry J. Campbell, Konstantina C. Fylaktakidou, Stamatia Papoutsopoulou
ng/mL) for 3 h. Medium was removed, cells were washed with PBS, and Bright-Glo lysis reagent added (
E2620
—
Promega
; Southampton, UK). After 5 min
incubation
at room temperature, lysates were transferred to 96-well
(12)
Cysteine Redox State Governs the Condensation Pathway of Hendra Virus W Protein and Differentially Impacts Type I IFN and NF-κB Signaling
Adv Sci (Weinh)
July 17, 2026
Frank Gondelaud, Alexandre Lalande, Giulia Pesce, Auriane Bouchet, Carole Meunier, Christophe Bignon, Denis Ptchelkine, Yu Gu, Eva Ogire, Pierre-Yves Lozach,
et al.
was measured after 5 h of
incubation
at 37°C using the
Bright‐Glo Luciferase Assay System
(
Promega
,
E2620
). The medium was replaced with 50 µL per well of DMEM/10% FBS, and 50 µL per well of Bright‐Glo Luciferase
(13)
Capsid and integrase play essential apposing roles in viral ribonucleoprotein assembly during HIV-1 core morphogenesis
iScience
July 17, 2026
Ahinsa Ranaweera, Jonathan R. Andino-Moncada, Sarah E. Dillon, Satya P. Singh, Scott M. Stagg, Alan N. Engelman, Christopher Aiken, Ashwanth C. Francis
assays
,Critical commercial
assays
,Critical commercial
assays
,, Bright-Glow
Luciferase Assay System
,
Promega
,Cat#
E2620
HIV1 p24 ELISA kit
,
Abcam
,Cat#
ab218268
,,
Experimental models
: Cell lines,
Experimental models
: Cell
(14)
Immunomodulatory Potential of Quinoline Q3, a Selective Inhibitor of the Canonical NF-κB Pathway in Macrophages
ChemMedChem
July 14, 2026
Konstantinos Michail, Panagiotis Ntavaroukas, Vasiliki Petriki, Evangelos Tsioupros, Stella Manta, Barry J. Campbell, Stamatia Papoutsopoulou
(PBS) pH 7.3 (Gibco) and luciferase activity was measured following
cell lysis
using a Bright‐Glo kit (
E2620
–
Promega
, Southampton, UK), as per manufacturer instructions. Cell lysates were transferred to 96‐well
(15)
Co-formulation of IL-12 mRNA and doxorubicin in polymeric nanoparticles for simultaneous delivery in murine melanoma
RSC Pharm
July 1, 2026
Elina Tanskanen, Hongning Sun, Kai-Chun Cheng, Jun Ishihara, Asha K. Patel
Pierce™ BCA Protein Assay Kit
(
23225
) were obtained from
Thermo Scientific
. A
Bright-Glo™ Luciferase Assay System
(
E2610
) and VivoGlo™ Luciferin (
P1041
) were obtained from
Promega
. Complete™ Protease Inhibitor Cocktail (04693116001)
(16)
Storage Buffer Composition Impacts Internal Structure, Freeze–Thaw Stability, and Transfection Efficiency of mRNA-Lipid Nanoparticles
ACS Nano
June 30, 2026
Meysam Mohammadi-Zerankeshi, Dylan J. Charland, Keira A. Donnelly, Geoffrey T. Nash, Jiale Shi, Dipak N. Patil, Julia Ennis, Kenneth G. Rodriguez, Sonia Corba, Noah A. Wambolt,
et al.
and cytotoxicities in
HepG2 cells
were evaluated by
Bright-Glo Luciferase Assay System
(
Promega
Cat#
E2610
) and CytoTox 96 Nonradioactive Cytotoxicity Assay Kit (
Promega
, Cat#
G1780
), respectively.
HepG2 cells
(17)
The structural mechanism of HIV-2 Vif antagonism of human APOBEC3H
Nature Communications
June 29, 2026
Yange Niu, Michelle Lilly, Estelle K. Ronayne, Michael Emerman, Nicholas M. Chesarino, John D. Gross
biological replicates. At 72 h post-
infection
, cells were lysed with 100 µL Bright-Glo reagent (
Promega
,
E2620
), and
luminescence
was measured using a
LUMIstar Omega
luminometer (
BMG Labtech
). The percent infectivity
(18)
First Structure–Activity-Relationship Study of Potent G2A Antagonists
Journal of Medicinal Chemistry
June 25, 2026
Victor Hernandez-Olmos, Jan Heering, Felix F. Lillich, Beatrice Marinescu, Sheila Nevermann, Johanna H. M. Ehrler, Dmytro S. Radchenko, Yurii S. Moroz, Astrid Kaiser, Andreas Krämer,
et al.
was aspirated, and the cells were lysed with 50 μL of a diluted bright-Glo reagent (
Promega
cat-no:
E2610
) in PBS (10× dilution). After a 15 min
incubation
with the lysis buffer,
luminescence
was measured using
(19)
Soyeom-Jetong mixture attenuates NLRP3 inflammasome-mediated inflammation
Frontiers in Pharmacology
June 16, 2026
Qianying Ye, Sojung Lee, Yeon-Cheol Park, Myung-Seo Kim, Wonnam Kim, Jong-Sik Jin, Dong-Keun Kim, Seoyeon Jang, Sumin Lee, Nahyun Lee,
et al.
mounting medium with
DAPI
(
ab104139
) were purchased from
Abcam
.
Bright-Glo™ luciferase assay system
(
E2610
) and pGL4.32 luciferase reporter vector (
E8491
) were procured from
Promega
.
Cell culture
and stimulation J774
(20)
DNA-based delivery of incretin receptor agonists using MYO Technology leads to durable weight loss in a diet-induced obesity model
Mol Ther Nucleic Acids
June 16, 2026
Linda Sasset, Andrew D. Cameron, Carleigh Sussman, Luisa Rubinelli, Debnath Maji, Robert Miller, Andy Thompson, Delcora Campbell, Melanie R. Walker, Marek M. Drozdz,
et al.
IRAs. After 3 h of
incubation
, cells were lysed with
Bright-Glo Luciferase Assay System
(
Promega
, cat#
E2610
) according to the manufacturer’s protocol, and the
luminescence
was measured using GloMax Navigator
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Bright-Glo Luciferase Assay System from Promega
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