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Bright-Glo Luciferase Assay System
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Bright-Glo Luciferase Assay System
Bright-Glo Luciferase Assay System
Bright-Glo Luciferase Assay System from Promega
Be the first to write a review!
Citations:
(587)
Figures:
(4)
Supplier Page
Supplier Page from
Promega for
Bright-Glo Luciferase Assay System
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Description
Bright-Glo Luciferase Assay System is designed for continuous-process systems. The robust, homogeneous assay chemistry gives high assay sensitivity and has a 30-minute signal half life
Product Specs
Company Info
Citations
Reviews
Product Reviews
Product Specs
Item
Bright-Glo Luciferase Assay System
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
E2610
Reactivity
Mammalian
Detection Target
Luciferase
Species
Mammalian
Sample Type
Mammalian cells
Method
Luciferase
Quantity
10ml
Sensitivity
Up to tenfold more light intensity than other homogeneous luciferase assay reagents results in increased sensitivity.
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Promega
2800 Woods Hollow Rd
Madison
,
WI
53711
United States
Phone
: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516
Email: custserv@promega.com
techserv@promega.com
Company Profile
Website:
http://www.promega.com
Citations (587)
(1)
Sialic acid cis-ligand dynamics modulate Siglec-7 and -9 function and affect Siglec-7/9 co-blockade to potentiate natural killer cell anti-tumor activity
Oncoimmunology
December 31, 2026
Jesper van Eck van der Sluijs, Anne H. C. Valk, Eline J. H. van Houtum, Esther D. Kers-Rebel, Maaike Looman, Willemijn Hobo, Anna E. Marneth, Harry Dolstra, Lune van den Boogaard, Christian Büll,
et al.
and 5% CO2, followed by
luminescence measurement
using
Bright-Glo™ Luciferase Assay System
(
Promega
,
E2620
) according to the manufacturer’s protocol on a Victor3 Multilabel Plate Reader (Perkin Elmer).
Western blot
(2)
Strain-specific epistasis shapes fitness landscapes of APOBEC3G antagonism by HIV-1 Vif proteins
Sci Adv
July 24, 2026
Caroline A. Langley, Michelle Lilly, Harmit S. Malik, Michael Emerman
DEAE-dextran (20 μg/ml). At 72 hours postinfection, cells were lysed in Bright-Glo reagent (
Promega
, #
E2610
), and luciferase activity was measured on a
LUMIstar Omega
luminometer (
BMG Labtech
). Each condition
(3)
Genetic diversity of pangolin coronaviruses reveals a key immuno-evasive substitution at spike residue 519
Journal of Virology
July 21, 2026
Maximilian Stanley Yo, Yu Kaku, Yusuke Kosugi, Jarel Elgin Tolentino, Daisuke Kuroda, Yunlong Cao, Kei Sato
post-
infection
, the infected cells were lysed with a BrightGlo luciferase
assay
system (
Promega
, Cat#
E2620
), and the luminescent signal produced by
firefly
luciferase reaction was measured using a GloMax Explorer
(4)
Prioritizing neuroactive ligands using motif-guided virtual discovery and zebrafish profiling
NPJ Drug Discov
July 21, 2026
Ari B. Ginsparg, Jaqueline A. Martinez, Ishaan Patel, Angélique Buton, Laura T. Lee, Raheel Sarwar, Rocco Moretti, Stephanie Puig, Summer B. Thyme
After
incubation
for 24 h at 37 °C, wells were treated with 5 μL of BrightGlo reagent (
Promega
- cat#
E2610
) and incubated in darkness at room temperature for 15 min. A back seal sticker (Perkin Elmer) was applied
(5)
Novel 3-Amino-2-methylquinazolinone NF-κB Inhibitors: Synthesis and Potential Anti-Inflammatory Function
Int J Mol Sci
July 20, 2026
Chrysoula Mikra, Vasiliki Petriki, Evangelos Tsioupros, Eleni Kougioumtzian, Konstantinos Michail, Stella Manta, Barry J. Campbell, Konstantina C. Fylaktakidou, Stamatia Papoutsopoulou
ng/mL) for 3 h. Medium was removed, cells were washed with PBS, and Bright-Glo lysis reagent added (
E2620
—
Promega
; Southampton, UK). After 5 min
incubation
at room temperature, lysates were transferred to 96-well
(6)
Cysteine Redox State Governs the Condensation Pathway of Hendra Virus W Protein and Differentially Impacts Type I IFN and NF-κB Signaling
Adv Sci (Weinh)
July 17, 2026
Frank Gondelaud, Alexandre Lalande, Giulia Pesce, Auriane Bouchet, Carole Meunier, Christophe Bignon, Denis Ptchelkine, Yu Gu, Eva Ogire, Pierre-Yves Lozach,
et al.
was measured after 5 h of
incubation
at 37°C using the
Bright‐Glo Luciferase Assay System
(
Promega
,
E2620
). The medium was replaced with 50 µL per well of DMEM/10% FBS, and 50 µL per well of Bright‐Glo Luciferase
(7)
Capsid and integrase play essential apposing roles in viral ribonucleoprotein assembly during HIV-1 core morphogenesis
iScience
July 17, 2026
Ahinsa Ranaweera, Jonathan R. Andino-Moncada, Sarah E. Dillon, Satya P. Singh, Scott M. Stagg, Alan N. Engelman, Christopher Aiken, Ashwanth C. Francis
assays
,Critical commercial
assays
,Critical commercial
assays
,, Bright-Glow
Luciferase Assay System
,
Promega
,Cat#
E2620
HIV1 p24 ELISA kit
,
Abcam
,Cat#
ab218268
,,
Experimental models
: Cell lines,
Experimental models
: Cell
(8)
Immunomodulatory Potential of Quinoline Q3, a Selective Inhibitor of the Canonical NF-κB Pathway in Macrophages
ChemMedChem
July 14, 2026
Konstantinos Michail, Panagiotis Ntavaroukas, Vasiliki Petriki, Evangelos Tsioupros, Stella Manta, Barry J. Campbell, Stamatia Papoutsopoulou
(PBS) pH 7.3 (Gibco) and luciferase activity was measured following
cell lysis
using a Bright‐Glo kit (
E2620
–
Promega
, Southampton, UK), as per manufacturer instructions. Cell lysates were transferred to 96‐well
(9)
Co-formulation of IL-12 mRNA and doxorubicin in polymeric nanoparticles for simultaneous delivery in murine melanoma
RSC Pharm
July 1, 2026
Elina Tanskanen, Hongning Sun, Kai-Chun Cheng, Jun Ishihara, Asha K. Patel
Pierce™ BCA Protein Assay Kit
(
23225
) were obtained from
Thermo Scientific
. A
Bright-Glo™ Luciferase Assay System
(
E2610
) and VivoGlo™ Luciferin (
P1041
) were obtained from
Promega
. Complete™ Protease Inhibitor Cocktail (04693116001)
(10)
Storage Buffer Composition Impacts Internal Structure, Freeze–Thaw Stability, and Transfection Efficiency of mRNA-Lipid Nanoparticles
ACS Nano
June 30, 2026
Meysam Mohammadi-Zerankeshi, Dylan J. Charland, Keira A. Donnelly, Geoffrey T. Nash, Jiale Shi, Dipak N. Patil, Julia Ennis, Kenneth G. Rodriguez, Sonia Corba, Noah A. Wambolt,
et al.
and cytotoxicities in
HepG2 cells
were evaluated by
Bright-Glo Luciferase Assay System
(
Promega
Cat#
E2610
) and CytoTox 96 Nonradioactive Cytotoxicity Assay Kit (
Promega
, Cat#
G1780
), respectively.
HepG2 cells
(11)
First Structure–Activity-Relationship Study of Potent G2A Antagonists
Journal of Medicinal Chemistry
June 25, 2026
Victor Hernandez-Olmos, Jan Heering, Felix F. Lillich, Beatrice Marinescu, Sheila Nevermann, Johanna H. M. Ehrler, Dmytro S. Radchenko, Yurii S. Moroz, Astrid Kaiser, Andreas Krämer,
et al.
was aspirated, and the cells were lysed with 50 μL of a diluted bright-Glo reagent (
Promega
cat-no:
E2610
) in PBS (10× dilution). After a 15 min
incubation
with the lysis buffer,
luminescence
was measured using
(12)
Soyeom-Jetong mixture attenuates NLRP3 inflammasome-mediated inflammation
Frontiers in Pharmacology
June 16, 2026
Qianying Ye, Sojung Lee, Yeon-Cheol Park, Myung-Seo Kim, Wonnam Kim, Jong-Sik Jin, Dong-Keun Kim, Seoyeon Jang, Sumin Lee, Nahyun Lee,
et al.
mounting medium with
DAPI
(
ab104139
) were purchased from
Abcam
.
Bright-Glo™ luciferase assay system
(
E2610
) and pGL4.32 luciferase reporter vector (
E8491
) were procured from
Promega
.
Cell culture
and stimulation J774
(13)
DNA-based delivery of incretin receptor agonists using MYO Technology leads to durable weight loss in a diet-induced obesity model
Mol Ther Nucleic Acids
June 16, 2026
Linda Sasset, Andrew D. Cameron, Carleigh Sussman, Luisa Rubinelli, Debnath Maji, Robert Miller, Andy Thompson, Delcora Campbell, Melanie R. Walker, Marek M. Drozdz,
et al.
IRAs. After 3 h of
incubation
, cells were lysed with
Bright-Glo Luciferase Assay System
(
Promega
, cat#
E2610
) according to the manufacturer’s protocol, and the
luminescence
was measured using GloMax Navigator
(14)
Commensal-derived acetylcholine enhances mucosal immune education
Nature
June 3, 2026
Deguang Song, Brianna Duncan-Lowey, Varnica Khetrapal, Randy Hamchand, Tong Deng, Hailey Brown, Anchi Wu, Anjelica L. Martin, Kaylyn M. Bauer, Yanyu Zhao,
et al.
the cell supernatant was discarded, and 50 µl of Bright-Glo luciferase substrate solution (
Promega
,
E2620
) was added to each well. After 15 min of
incubation
at room temperature,
luminescence
was measured using
(15)
Identification of a unique humoral immune signature of Sudan ebolavirus persistence in human survivors
Research Square
May 27, 2026
Shuangyi Gunn, Shuangyi Muwonge, Carolyne Nasimiyu, Carolyne Bakamutumaho, Barnabas Bakamutumaho, Cynthia Ulusan, Peter James Ulusan, Moses L. Elyanu, Robert F. Breiman, Robert Ssewanyana,
et al.
activity was measured by adding 30 μl per well of Bright-Glo luciferase substrate (
Promega
, cat. #.
E2620
) and reading
luminescence
on a Tecan Spark luminometer (Life Sciences). Percent neutralization was calculated
(16)
A targetable opioid/cancer associated fibroblast axis drives extracellular matrix remodeling and tumor aggressiveness in pancreatic cancer
bioRxiv
May 26, 2026
Kathryn E. Maraszek, Arwen A. Tisdale, Hunter D. Reavis, Xiaozhuo Liu, Eduardo Cortes Gomez, Aleksandr Dolskii, Caneta Brown, Daksh Thakkar, Maura Dungan, Anusha Adhikari,
et al.
the respective conditions. On day 5, the
Promega
Bright-Glo Luciferase Assay System
(
Promega
, cat. #
E2610
) was used to
measure
the
luminescence
of each well according to the manufacturer’s protocol. Extra wells
(17)
Protease-activated receptor 1 as an endogenous model of peptidergic Gαq-Gα12-biased G protein signaling
Frontiers in Molecular Biosciences
May 25, 2026
Braden S. Fallon, Robert A. Campbell, Justin G. English
a
PHERAstar FSX
(BMG Labtech, RRID:SCR_027001) using the Bright-Glo Luciferase
Assay
(
Promega
, Cat#
E2610
). Bright-Glo reagent was diluted in an equal volume of
assay
buffer (20 mM HEPES in HBSS), and 10 μL
(18)
Light- and chemical-induced ciliary signaling governs dorsal/ventral regionalization of human telencephalic organoids
Nature Communications
May 22, 2026
Issei S. Shimada, Akari Goto, Yutaka Hashimoto, Hajime Inoue, Takuto Sugawara, Tomohiro Doura, Tsubasa Fujita, Toshiaki Iwata, Riko Shimmoto, Hiroshi Takase,
et al.
Chemical Industry, F0855) was used.
HEK293 cells
were utilized for Bright-Glo Luciferase
assay
(
Promega
,
E2620
) according to the manufacturer’s instructions. The signal was quantified with a
FlexStation3
(
Molecular Devices
(19)
TMPRSS2-induced Golgi disruption restricts the incorporation of virus envelope glycoproteins into virions
EMBO Reports
May 19, 2026
Sayuri Seki, Shigeyoshi Harada, Akiko Sugimoto-Ishige, Midori Unno, Machie Sakuma, Shinsuke Ito, Hiroyuki Yamamoto, Aki Tanabe, Saori Matsuoka, Chieko Makino-Okamura,
et al.
kit,ZeptoMetrix,0801008 Glo lysis buffer,
Promega
,
E2661
Bright-Glo luciferase assay system
,
Promega
,
E2620
carboxymethyl cellulose sodium salt,FujiFilm Wako,039-01335 neutral buffered formalin,Muto Chemical,20214 crystal
(20)
Algorithm-Optimized H5 Influenza mRNA Vaccine Induces Broad Immune Responses
Int J Mol Sci
May 19, 2026
Liangliang Wang, Zhengda Peng, Chenchen He, Jie Zhang, Pengju Yu, Weijin Huang, Youchun Wang, Chenyan Zhao
h. After removing the supernatant, luciferase activity was measured using a luciferase
assay
system (
E2620
,
Promega
) with 2 min of shaking.
Luminescence
was quantified using a PerkinElmer EnSight(Waltham, MA,
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Bright-Glo Luciferase Assay System from Promega
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