Nano-Glo Luciferase Assay N1110 from Promega

Product Specs
ItemNano-Glo Luciferase Assay
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberN1110
Quantity10ml

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Expanded applications of bioluminescence microscopy with phasor analysisCell Reports MethodsApril 20, 2026Lila P. Halbers, Caroline K. Brennan, Lorenzo Scipioni, Giulia Tedeschi, Zachary R. Torrey, Kshitij Parag-Sharma, Bryan Labra, Christoph Gohlke, Antonio L. Amelio, Michelle A. Digman, et al.10035-04-8 EGTA,Sigma Aldrich,CAS Number: 67-42-5 STS,VWR,CAS Number: 62996-74-1 Furimazine,Promega,Catalog number: N1110 Puromycin,–,– Coelenterazine,GoldBio,Catalog Number:CZ2.5 CAS Number:55779-48-1 Catalase from bovine liver

(2) Vacuum and Sonication Treatment Enable Efficient Transient Gene Expression in Various Monocot and Eudicot Plant SeedlingsACS Synthetic BiologyApril 17, 2026Eugene Li, Yuan Geng, Tuyako R. Khristoforova, Yunqing Wang, Jolie W. Jones, Kimberley T. Muchenje, Elisa Grillo, Hao Zhang, Robert J. Schmitz, Gozde S. Demirermin to pellet the cell debris. Luminescence was measured using the Nano-Glo Luciferase Assay System (N1110, Promega) according to the manufacturer’s instructions, with minor modifications. A 15 μL aliquot of

(3) Airway organoids reveal patterns of influenza A tropism and adaptation in wildlife speciesEmerg Microbes InfectApril 17, 2026Ferran Tarrés-Freixas, Gerardo Ceada, Francesc Català-Moll, Maria Casadellà, Sara Tolosa-Alarcón, Eloi Franco-Trepat, Rytis Boreika, Vanessa Almagro-Delgado, Noelia Carmona, Josep Estruch, et al.expression was assessed 72 h after SARS-CoV-2 inoculation using a Nano-Glo Luciferase Assay (Promega; #N1110) and measured with a Fluoroskan FL Plate Reader (Thermo Fisher). Supplementary Material

(4) CGM23 corresponds to a pan-coronavirus lipopeptide inhibitor potently inhibiting virion fusioniScienceApril 17, 2026Yusuke Matsui, Roland Schwarzer, Mauricio Montano, Ekram W. Helmy, Rahul K. Suryawanshi, Taha Y. Taha, Yan Wang, Yvonne Angel, Marc Adler, M. Flori Sassano, et al.DMEM was add per well followed by 100 μLs of Nano-Glo Luciferase substrate/buffer cocktail (Promega, N1110). The plates were incubated for 5 minutes at 37°C and 5 % CO2 then read on a GloMax Discover luminometer.

(5) Engineering of genetically encoded programmable calcium channel inhibitory bindersNat CommunApril 13, 2026Xiaoxuan Liu, Sher Ali, Tien-Hung Lan, Decheng Wang, Brendan McKee, Tatsuki Nonomura, Siyao Liu, Feng Zhao, Michael X. Zhu, Yun Huang, et al.96-well plates. Bioluminescence was initiated using the Nano-Glo® Luciferase Assay System (Promega, N1110), and emission signals were recorded on a Cytation 5 Cell Imaging Multi-Mode Reader (BioTek). Raw BRET

(6) U2AF regulates the translation and localization of nuclear-encoded mitochondrial mRNAsMol CellApril 2, 2026Gloria R. Garcia, Murali Palangat, Josquin Moraly, Benjamin T. Donovan, Bixuan Wang, Ira Phadke, David Sturgill, Jiji Chen, Guofeng Zhang, Ronald J. Holewinski, et al.Concentrator Kit,Zymo,D4003 HiScribe SP6 RNA synthesis kit,NEB,E2070S Nano-Glo Luciferase Assay,Promega,N1110 MitoTracker Red CMXRos,Invitrogen,2351994 FoxP3 Staining Buffer Set,Thermo Fisher,00-5523-00 ImProm-II reverse transcription system

(7) A dual-reporter mouse for therapeutic discovery in Angelman syndromeJCI InsightMarch 9, 2026Hanna Vihma, Lucas M. James, Hannah C. Nourie, Audrey L. Smith, Siyuan Liang, Carlee A. Friar, Tasmai Vulli, Lei Xing, Dale O. Cowley, Alain C. Burette, et al.substrate per well, followed by luminescence measurement using the Nano-Glo Luciferase Assay System (N1110, Promega), according to the manufacturers’ protocols. Fluorescence and luminescence were measured on

(8) An innovative nasal nanovaccine against SARS-CoV-2 induces systemic and upper airway immunity controlling viral replicationnpj VaccinesMarch 4, 2026Roberta L. Pagni, Edecio Cunha-Neto, Yasmin da Silva Santos, Edilberto Postól, Raquel Elaine de Alencar, Ana Moretti, Jonnatan J. Santos, Tamires L. Silva, Isabela P. Daher, Marcos C. Knirsch, et al.Cell Culture Lysis reagent (#E1500, Promega), and luciferase activity was measured using the Nano-Glo system (#N1110, Promega) in a luminometer (GloMax® Navigator Microplate Luminometer). The 50% inhibitory concentration

(9) A Sensitive Reporter Mouse Model to Study Adipocyte‐Derived Extracellular Vesicles In VivoJournal of Extracellular VesiclesFebruary 19, 2026Didde Riisager Hansen, Rugivan Sabaratnam, Lasse Bach Steffensen, Per Svenningsenfluids, and cell‐conditioned medium was measured using the Nano‐Glo Luciferase Assay System (Promega, N1110). Luminescence was measured in Relative Light Units per second (RLU/s) using the Orion L Microplate

(10) Negative feedback regulation of STING signaling by TAX1BP1-directed GolgiphagyNature CommunicationsFebruary 11, 2026Sujit Suklabaidya, Suchitra Mohanty, Irene E. Reider, Jesse White, Dominic Colter, Sarah M. McCormick, Noula Shembade, Young Bong Choi, Christopher C. Norbury, Edward W. Harhajin triplicate and treated with 25 µl of Opti-MEM I medium supplemented with 1% furimazine (Promega; N1110) for 5 min as described previously35. Luminescence intensities were measured using a GloMax 96 microplate luminometer

(11) Enhancing Circular RNA Translation Efficiency Through Dual Internal Ribosome Entry SitesBiology (Basel)February 11, 2026Yawen Sun, Yimin Zhang, Weijie Chen, Ting Chen, Yunlong Zhang, Shanyu Zhang, Changrui Luroom temperature for 15 min. Nano-Glo® Luciferase Assay Reagent (Promega, Madison, WI, USA; Cat. No. N1110) was prepared according to the manufacturer’s instructions, and 100 µL was added to each well. The plates

(12) Development of a high-throughput screening platform for C. difficile toxin synthesis inhibitors unveils meclizine as an antivirulence agentAntimicrobial Agents and ChemotherapyFebruary 4, 2026Ravi K. R. Marreddy, Nghi Nguyen, Chetna Dureja, Ann Marie McKelvey, Reid Powell, Abiola O. Olaitan, Clifford Stephan, Julian G. Hurdleactivity was measured by mixing culture supernatant and NanoGlo luciferase substrate (Promega Cat. No. N1110) and measuring luminescence using a Cytation5 plate reader from Biotek; luminescence readings were normalized

(13) A single valine to leucine switch disrupts Plasmodium falciparum AP2-G DNA binding and reveals GDV1’s role in ap2-g activationNature CommunicationsJanuary 17, 2026Surendra K. Prajapati, Jeffrey X. Dong, Belinda J. Morahan, Thoai Dotrang, Michelle C. Barbeau, April E. Williams, Daniel Hupalo, Matthew D. Wilkerson, Clifton L. Dalgard, Bjorn F. C. Kafsack, et al.system Nanoluciferase (nLuc) bioluminescence signal was measured using a Nano-Glo® Luciferase Assay System (Cat# N1110, Promega, USA) and the Cytation 5 Biotek imaging system as per manufacturer’s instruction. Gen 5 v3.08

(14) Development of a Thermostable and Broadly Neutralizing Pan-Sarbecovirus Vaccine CandidateACS Infectious DiseasesJanuary 9, 2026Simran Srivastava, Sahil Kumar, Suman Mishra, Raju S. Rajmani, Randhir Singh, Somnath Dutta, Rajesh Prakash Ringe, Raghavan Varadarajanluciferase activity in the cells (relative luminescence units) using Nano-Glo luciferase substrate (Cat # N1110, Promega). Luminescence was measured using a Cytation-5 multimode reader (Bio-Tech Inc., Oklahoma City,

(15) Dynamics and biodistribution of cardiomyocyte-derived small extracellular vesicles following myocardial infarction: sex differencesAmerican Journal of Physiology-Heart and Circulatory PhysiologyJanuary 1, 2026Xinjie Wang, Xiao Du, Zhishi Chen, Wei Yu, Zhongjing Wang, Yanping Chen, Daniel Y. Chang, Garrett Jensen, Weijia Luo, Jiang Changwere lysed with 100 μL passive lysis buffer. 20 μL lysate was reacted with 20 μL NanoLuc substrate (N1110, Promega) for Bioluminescence reading (Lumat3 LB9508, Berthold). Relative luciferase unit reading was

(16) In silico docking yields small molecule negative allosteric modulators targeting the core of Frizzled 7Nat CommunDecember 14, 2025Magdalena M. Scharf, Julia Kinsolving, Lukas Grätz, Jan Hendrik Voss, David Carrasco-Busturia, Björn Forsberg, Peter Kolb, Gunnar Schulteadditional CO2), furimazine (for Nluc-FZD7, diluted 1/1000 in HBSS; Nano-Glo substrate, Promega, cat.-No.: N1110) or a mix of LgBiT and furimazine (for HiBiT-FZD7, both diluted 1/200 in HBSS, Promega, cat.-No.: N2421)

(17) Pathogenic variants in KCTD1 disrupt cAMP signaling and cellular communication associated with developmental pathwaysJournal of Biological ChemistryDecember 1, 2025Yini Liao, Brian S. Munteanseparate well containing from 20 μg total lysate after addition of 2× Nano-Glo substrate (Promega #N1110) in lysis buffer. IP was performed by mixing 300 μg of total lysate with 10 μl Pierce Anti-DYKDDDDK

(18) Strategies to Screen and Evaluate Brain Targeting Antibodies Using an iPSC-Derived Blood–Brain Barrier ModelAntibodies (Basel)November 26, 2025Eun Seo Choi, Sophia Sahota, Emily Burnham, Yunfeng Ding, Eric V. Shustatranscytosed scFv-Fc-nLuc were collected for analysis. The Nano-Glo® Luciferase Assay System (Promega N1110) was used to generate the luminescence signal with the nLuc enzyme upon addition of the furimazine substrate,

(19) Regulation of therapeutic protein release in response to circadian biomarkersNature CommunicationsNovember 6, 2025Nik Franko, Shichao Li, Silvia Galvan, Zsoka Csorba, Ana Palma Teixeira, Mingqi Xie, Martin Fusseneggerexpression levels were quantified in cell culture supernatants with the Nano-Glo Luciferase Assay System (N1110, Promega). Briefly, 10 μL of buffer/substrate mixture (prepared at a 50:1 ratio) was mixed with 10 μL

(20) Production of Genetically Engineered Extracellular Vesicles for Targeted Protein DeliveryBIO-PROTOCOLNovember 5, 2025Leyla A. Ovchinnikova, Evgeniy G. Evtushenko, Dmitriy V. Bagrov, Anton O. Goncharov, Alexander G. Gabibov, Yakov A. Lomakinsolution (100×) (Gibco, catalog number: 11140035) 18. Nano-Glo luciferase assay (Promega, catalog number: N1110) 19. NEBuilder HiFi DNA Assembly Cloning kit (NEB, catalog number: E5520S) 20. Opti-MEMTM reduced serum

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Nano-Glo Luciferase Assay

Nano-Glo Luciferase Assay from Promega

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Nano-Glo Luciferase Assay from Promega

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