Nano-Glo Luciferase Assay N1110 from Promega

Product Specs
ItemNano-Glo Luciferase Assay
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberN1110
Quantity10ml

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Systemic Propagation of STING Signalling via Generation of Large Extracellular VesiclesJ Extracell VesiclesMay 9, 2026Jiae Lee, Annabel Vernon, Hyung Joon Park, Young V. Kwonplate with one well between samples to reduce cross talk. Nano‐Glo Luciferase Assay System (Promega, N1110) was used as directed to detect luminescence. Luminescence was measured using a SpectraMax i3x (Molecular Devices

(2) A whole-animal phenotypic drug screen identifies suppressors of atherogenic lipoproteinsbioRxivMay 5, 2026Daniel J. Kelpsch, Liyun Zhang, James H. Thierer, Kobe Koren, Urmi Kumar, Yuki Lin, Monica R. Hensley, Mira Sohn, Jun O. Liu, Thomas Lectka, et al.light:dark cycle for 48 hours. At 5 dpf, a solution of anesthetic, fixative, and NanoGlo substrate (Promega N1110) was added to each well of each drug plate at a final concentration of 0.014% MESAB/4.6% paraformaldehyde

(3) Expanded applications of bioluminescence microscopy with phasor analysisCell Reports MethodsApril 20, 2026Lila P. Halbers, Caroline K. Brennan, Lorenzo Scipioni, Giulia Tedeschi, Zachary R. Torrey, Kshitij Parag-Sharma, Bryan Labra, Christoph Gohlke, Antonio L. Amelio, Michelle A. Digman, et al.10035-04-8 EGTA,Sigma Aldrich,CAS Number: 67-42-5 STS,VWR,CAS Number: 62996-74-1 Furimazine,Promega,Catalog number: N1110 Puromycin,–,– Coelenterazine,GoldBio,Catalog Number:CZ2.5 CAS Number:55779-48-1 Catalase from bovine liver

(4) Microprotein Regulates G-quadruplex Driven RNA AggregationbioRxivApril 18, 2026Bikash R. Sahoo, Janakraj Bhattrai, Arnav Sharma, Ursula Jakob, James CA Bardwellfor 30 min in a 96-well format. Luciferase activity was measured using NanoGlo substrate (Promega, N1110) on a Tecan M1000 plate reader. Translation products were further analyzed by anti-FLAG immunoblotting

(5) Airway organoids reveal patterns of influenza A tropism and adaptation in wildlife speciesEmerg Microbes InfectApril 17, 2026Ferran Tarrés-Freixas, Gerardo Ceada, Francesc Català-Moll, Maria Casadellà, Sara Tolosa-Alarcón, Eloi Franco-Trepat, Rytis Boreika, Vanessa Almagro-Delgado, Noelia Carmona, Josep Estruch, et al.expression was assessed 72 h after SARS-CoV-2 inoculation using a Nano-Glo Luciferase Assay (Promega; #N1110) and measured with a Fluoroskan FL Plate Reader (Thermo Fisher). Supplementary Material

(6) CGM23 corresponds to a pan-coronavirus lipopeptide inhibitor potently inhibiting virion fusioniScienceApril 17, 2026Yusuke Matsui, Roland Schwarzer, Mauricio Montano, Ekram W. Helmy, Rahul K. Suryawanshi, Taha Y. Taha, Yan Wang, Yvonne Angel, Marc Adler, M. Flori Sassano, et al.DMEM was add per well followed by 100 μLs of Nano-Glo Luciferase substrate/buffer cocktail (Promega, N1110). The plates were incubated for 5 minutes at 37°C and 5 % CO2 then read on a GloMax Discover luminometer.

(7) Vacuum and Sonication Treatment Enable Efficient Transient Gene Expression in Various Monocot and Eudicot Plant SeedlingsACS Synthetic BiologyApril 17, 2026Eugene Li, Yuan Geng, Tuyako R. Khristoforova, Yunqing Wang, Jolie W. Jones, Kimberley T. Muchenje, Elisa Grillo, Hao Zhang, Robert J. Schmitz, Gozde S. Demirermin to pellet the cell debris. Luminescence was measured using the Nano-Glo Luciferase Assay System (N1110, Promega) according to the manufacturer’s instructions, with minor modifications. A 15 μL aliquot of

(8) Engineering of genetically encoded programmable calcium channel inhibitory bindersNat CommunApril 13, 2026Xiaoxuan Liu, Sher Ali, Tien-Hung Lan, Decheng Wang, Brendan McKee, Tatsuki Nonomura, Siyao Liu, Feng Zhao, Michael X. Zhu, Yun Huang, et al.96-well plates. Bioluminescence was initiated using the Nano-Glo® Luciferase Assay System (Promega, N1110), and emission signals were recorded on a Cytation 5 Cell Imaging Multi-Mode Reader (BioTek). Raw BRET

(9) U2AF regulates the translation and localization of nuclear-encoded mitochondrial mRNAsMol CellApril 2, 2026Gloria R. Garcia, Murali Palangat, Josquin Moraly, Benjamin T. Donovan, Bixuan Wang, Ira Phadke, David Sturgill, Jiji Chen, Guofeng Zhang, Ronald J. Holewinski, et al.Concentrator Kit,Zymo,D4003 HiScribe SP6 RNA synthesis kit,NEB,E2070S Nano-Glo Luciferase Assay,Promega,N1110 MitoTracker Red CMXRos,Invitrogen,2351994 FoxP3 Staining Buffer Set,Thermo Fisher,00-5523-00 ImProm-II reverse transcription system

(10) A dual-reporter mouse for therapeutic discovery in Angelman syndromeJCI InsightMarch 9, 2026Hanna Vihma, Lucas M. James, Hannah C. Nourie, Audrey L. Smith, Siyuan Liang, Carlee A. Friar, Tasmai Vulli, Lei Xing, Dale O. Cowley, Alain C. Burette, et al.substrate per well, followed by luminescence measurement using the Nano-Glo Luciferase Assay System (N1110, Promega), according to the manufacturers’ protocols. Fluorescence and luminescence were measured on

(11) An innovative nasal nanovaccine against SARS-CoV-2 induces systemic and upper airway immunity controlling viral replicationnpj VaccinesMarch 4, 2026Roberta L. Pagni, Edecio Cunha-Neto, Yasmin da Silva Santos, Edilberto Postól, Raquel Elaine de Alencar, Ana Moretti, Jonnatan J. Santos, Tamires L. Silva, Isabela P. Daher, Marcos C. Knirsch, et al.Cell Culture Lysis reagent (#E1500, Promega), and luciferase activity was measured using the Nano-Glo system (#N1110, Promega) in a luminometer (GloMax® Navigator Microplate Luminometer). The 50% inhibitory concentration

(12) A Sensitive Reporter Mouse Model to Study Adipocyte‐Derived Extracellular Vesicles In VivoJournal of Extracellular VesiclesFebruary 19, 2026Didde Riisager Hansen, Rugivan Sabaratnam, Lasse Bach Steffensen, Per Svenningsenfluids, and cell‐conditioned medium was measured using the Nano‐Glo Luciferase Assay System (Promega, N1110). Luminescence was measured in Relative Light Units per second (RLU/s) using the Orion L Microplate

(13) Negative feedback regulation of STING signaling by TAX1BP1-directed GolgiphagyNature CommunicationsFebruary 11, 2026Sujit Suklabaidya, Suchitra Mohanty, Irene E. Reider, Jesse White, Dominic Colter, Sarah M. McCormick, Noula Shembade, Young Bong Choi, Christopher C. Norbury, Edward W. Harhajin triplicate and treated with 25 µl of Opti-MEM I medium supplemented with 1% furimazine (Promega; N1110) for 5 min as described previously35. Luminescence intensities were measured using a GloMax 96 microplate luminometer

(14) Enhancing Circular RNA Translation Efficiency Through Dual Internal Ribosome Entry SitesBiology (Basel)February 11, 2026Yawen Sun, Yimin Zhang, Weijie Chen, Ting Chen, Yunlong Zhang, Shanyu Zhang, Changrui Luroom temperature for 15 min. Nano-Glo® Luciferase Assay Reagent (Promega, Madison, WI, USA; Cat. No. N1110) was prepared according to the manufacturer’s instructions, and 100 µL was added to each well. The plates

(15) Development of a high-throughput screening platform for C. difficile toxin synthesis inhibitors unveils meclizine as an antivirulence agentAntimicrobial Agents and ChemotherapyFebruary 4, 2026Ravi K. R. Marreddy, Nghi Nguyen, Chetna Dureja, Ann Marie McKelvey, Reid Powell, Abiola O. Olaitan, Clifford Stephan, Julian G. Hurdleactivity was measured by mixing culture supernatant and NanoGlo luciferase substrate (Promega Cat. No. N1110) and measuring luminescence using a Cytation5 plate reader from Biotek; luminescence readings were normalized

(16) A single valine to leucine switch disrupts Plasmodium falciparum AP2-G DNA binding and reveals GDV1’s role in ap2-g activationNature CommunicationsJanuary 17, 2026Surendra K. Prajapati, Jeffrey X. Dong, Belinda J. Morahan, Thoai Dotrang, Michelle C. Barbeau, April E. Williams, Daniel Hupalo, Matthew D. Wilkerson, Clifton L. Dalgard, Bjorn F. C. Kafsack, et al.system Nanoluciferase (nLuc) bioluminescence signal was measured using a Nano-Glo® Luciferase Assay System (Cat# N1110, Promega, USA) and the Cytation 5 Biotek imaging system as per manufacturer’s instruction. Gen 5 v3.08

(17) pH-responsive synthetic cells for controlled protein synthesis and releasebioRxivJanuary 16, 2026Sung-Won Hwang, Yudan Li, Alexander A. Green, Allen P. Liua gift from Prof. Sungmin Nam (University of Michigan). Nano-Glo® luciferase assay substrate (Cat# N1110) was purchased from Promega (Madison, WI, USA). Ni-charged magnetic beads (Cat# L00295) were purchased

(18) Development of a Thermostable and Broadly Neutralizing Pan-Sarbecovirus Vaccine CandidateACS Infectious DiseasesJanuary 9, 2026Simran Srivastava, Sahil Kumar, Suman Mishra, Raju S. Rajmani, Randhir Singh, Somnath Dutta, Rajesh Prakash Ringe, Raghavan Varadarajanluciferase activity in the cells (relative luminescence units) using Nano-Glo luciferase substrate (Cat # N1110, Promega). Luminescence was measured using a Cytation-5 multimode reader (Bio-Tech Inc., Oklahoma City,

(19) Dynamics and biodistribution of cardiomyocyte-derived small extracellular vesicles following myocardial infarction: sex differencesAmerican Journal of Physiology-Heart and Circulatory PhysiologyJanuary 1, 2026Xinjie Wang, Xiao Du, Zhishi Chen, Wei Yu, Zhongjing Wang, Yanping Chen, Daniel Y. Chang, Garrett Jensen, Weijia Luo, Jiang Changwere lysed with 100 μL passive lysis buffer. 20 μL lysate was reacted with 20 μL NanoLuc substrate (N1110, Promega) for Bioluminescence reading (Lumat3 LB9508, Berthold). Relative luciferase unit reading was

(20) De novo design of protein competitors for small molecule immunosensingbioRxivDecember 19, 2025Yosta de Stigter, Tallie Godschalk, Maarten Merkxincubated for 30 – 60 minutes at room temperature. Subsequently, 2 μL NLuc substrate furimazine (Promega, N1110) was added to a final 1,000-fold dilution and the bioluminescent emission at 458 nm was recorded in

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Nano-Glo Luciferase Assay

Nano-Glo Luciferase Assay from Promega

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Nano-Glo Luciferase Assay from Promega

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