Nano-Glo Luciferase Assay N1110 from Promega

Product Specs
ItemNano-Glo Luciferase Assay
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberN1110
Quantity10ml

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Sentinel plants enable quantitative monitoring of bioavailable nitrate in soils and microbial environmentsProc Natl Acad Sci U S AJuly 28, 2026Eugene Li, Chiara Berruto, Tufan M. Oz, Elisa Grillo, Catherine Griffin, Yunqing Wang, Kimberley T. Muchenje, Jolie W. Jones, Gozde S. DemirerTecan Spark (100-ms integration). GeNL/NLuc was measured with the Nano-Glo Luciferase Assay System (N1110, Promega; GeNL 520 to 590 nm, NLuc 430 to 485 nm) and the ELuc/RedF pair with the Luciferase Assay System

(2) Synthetic Toll-Like Receptors for Control of Innate Immunity With Far-Red LightAdv Sci (Weinh)July 27, 2026Anna V. Leopold, Vladislav V. Verkhushahalf‐area white plates (Costar) by mixing 10 µL lysate with 20 µL Nano‐Glo substrate (Promega, Cat. #N1110) and Firefly Luciferase activity was measured in 96‐well half‐area white plates (Costar) by mixing 10

(3) Food additives with aldehyde skeleton exhibited leptin resistance in cellsFront NutrJuly 23, 2026Yuki Shibuya, Haruto Yoshida, Kyoshiro Tsuge, Yu Hamasaki, Karen Kuriya, Tadashi Nakagawa, Aoi Sugiyama, Kazuhide Miyamoto, Akira Shimamoto, Ralf Jockers, et al.the leptin. The measurement was done according to the Nano-Glo Luciferase Assay procedure (Promega N1110) using the Infinite® 200 PRO (TECAN). Cell viability assay The cells were seeded at 80,000 cells/well,

(4) Programmable Nanobody-Targeting Chimeras Enable Intracellular Viral Protein DegradationAdv Sci (Weinh)July 17, 2026Max Yu-Chen Pan, Ting-Hui Lee, Tzu-Ning Peng, Eva Yi-Hsuan Wu, Yu-Ting Chiu, Chih-Chien Chiu, Hong Vinh Nguyen, Yuan-Shao Pao, Ming-Hong Chao, Richard Kuan-Lin Lee, et al.Nano‐Glo luciferase assay substrate, and 9.5 µL of luciferase assay diluent (Promega, WI, USA. Cat.# N1110) was added into each well. The luminescence signal was recorded every 2 min continuously for 30 min

(5) K63-linked ubiquitin chains mark inactive Smoothened for packaging into ciliary extracellular vesiclesbioRxivJuly 17, 2026Mingli Zhu 祝明莉, Yash Raj, Gary A. Bradshaw, David U. Mick, Marian Kalocsay, Maxence V. NachuryAfter 24 h, a NanoLuciferase assay was performed with a Nano-Glo® Luciferase Assay System (Promega N1110) according to the manufacturer’s instructions. As diagramed in Fig. 3A, starvation medium and cells

(6) Dynamic assembly of malate dehydrogenase–citrate synthase multienzyme complex in the mitochondriaElifeJuly 8, 2026Joy Omini, Inga Krassovskaya, Taiwo Adeolu Dele-Osibanjo, Connor Pedersen, Toshihiro Obatasequences are provided in Appendix 1 Commercial assay or kit,Nano-Glo Luciferase Assay System,Promega,N1110,Furimazine substrate for NanoBiT/NanoLUC luminescence assays Commercial assay or kit,Protein Labeling

(7) An Optimized Protocol for the Characterization of Zebrafish ApoB-Containing Lipoproteins Using the LipoGlo SystemBio ProtocJuly 5, 2026Monica R. Hensley, Steven A. FarberNano-Glo® luciferase assay substrate and Nano-Glo® luciferase assay buffer) (Promega, catalog number: N1110) 15. OptiPrep density gradient medium (Sigma, catalog number: D1556) 16. Phosphate buffer saline (PBS)

(8) CD46 regulates hepatitis B virus entry by modulating cell-surface NTCP levels through cis-interactionJournal of Molecular Cell BiologyJuly 3, 2026Kei Miyakawa, Yusuke Nakai, Taichi Kameya, Hironori Nishitsuji, Koichi Watashi, Makoto Takeda, Tsukasa Seya, Kunitada Shimotohno, Yayoi Kimura, Akihide Ryosecreted luciferase activity in the culture supernatant using Nano-Glo Luciferase Assay System (Promega, #N1110) according to the manufacturer’s instructions. For wild-type HBV infections, viral replication was assessed

(9) Calcium and phosphorylation coordination is a novel mechanism that stabilises protein-complexes during HIV assemblyJ Biomed SciJuly 2, 2026Erwan Brémaud, Biswa Prasanna Mishra, Ablikim Baqi, Veronika Masic, Thibaut Flipo, Arun Everest-Dass, Lauren Hartley-Tassell, Paul R. Gorry, Thomas Ve, Belinda L. Spillings, et al.buffer. The efficiency of NanoLuc delivery was measured using Nano-Glo Luciferase Assay System (Promega, N1110) according to manufacturer’s instructions. After 3 min, samples were analysed in Costar 96 flat bottom,

(10) Harnessing extracellular vesicles for stabilized and functional IL-10 delivery in macrophage immunomodulationExtracellular VesicleJune 29, 2026Najla A. Saleh, Matthew A. Gagea, Xheneta Vitija, Sadhana Kilangodi, Ahmed A. Zarea, Tomas Janovic, Jens C. Schmidt, Cheri X. Deng, Masamitsu Kanadaand NF-κB activity were analyzed by measuring bioluminescence signals. Furimazine (25 μM; Promega, N1110) was added for PalmReNL detection, and D-luciferin (300 μg/mL; Promega, E160A) was added for the NF-κB

(11) Asialoglycoprotein receptor subunit asgr1a loss results in attenuated cholesterol absorption in zebrafish fed a Western dietJournal of Biological ChemistryJune 9, 2026Joshua T. Derrick, Tabea O.C. Moll, Darby W. Sweeney, Jeffrey Shin, Monika Svecla, Giuseppe Danilo Norata, Steven A. Farber(56). LipoGlo assays See (56) for detailed LipoGlo methods. All reagents were obtained from Promega Corp., (N1110; (36)). Larval, juvenile, and adult asgr1ac830 mutants, as well as adult asgr1ac865 mutants carried

(12) Extracellular ATP drives tryptophan metabolism and aryl hydrocarbon receptor activation to promote cellular senescenceJournal of Biological ChemistryJune 1, 2026Daniela Volonte, Steven J. Mullett, Stacy L. Gelhaus, Ferruccio Galbiatito quantify secreted NanoLuc luciferase using the Nano-Glo Luciferase Assay System (catalog number N1110, Promega) (97). Results were normalized based on protein concentration, as determined using the BCA

(13) The benchmarking and application of tag-degraders in vivo to validate therapeutic targetsNature CommunicationsMay 29, 2026Charlene M. Magtoto, Stephen Mieruszynski, Hao Dong, Ashley P. Ng, Ladina Di Rago, Andrew J. Kueh, Martin Brzozowski, Christoph Grohmann, Joel R. Walker, Ngee Kiat Chua, et al.assays NanoLuc luminescence induction was performed by preparing a master mix of Nano-GLO reagent (Promega, N1110) at a substrate to buffer ratio of 1:50. Normalised protein lysates and the Nano-GLO master mix were

(14) Toward a Small Molecule Therapy for Angelman Syndrome: Structure–Activity Relationship Studies of 3-Aminopyrazole Phenylacetamides as Paternal Ube3a UnsilencersJournal of Medicinal ChemistryMay 28, 2026Anna Welton-Arndt, Hannah C. Nourie, Hanna Vihma, Siyuan Liang, Jonathan Super, Kelin Li, Jon L. Collins, Hsueh-Cheng Huang, Allison Zhang, Ashley E. Trojniak, et al.and an emission detection of 505–10 nm. Luminescence was then assessed by adding Nano-Glo® reagent (N1110, Promega) as per manufacturer protocol, with 25 μL of prepared substrate added to each well. Plates

(15) Dysfunction of a SET3-like complex underlies a family of related neurological disordersNature CommunicationsMay 16, 2026Katie M. Paton, Beatrice Alexander-Howden, Jenna I. Hare, Jacky Guy, Kashyap Chhatbar, Maria Yudina, Stanley T. Morris, Robyn Walls, Tricia Mathieson, Christos Spanos, et al.were prepared and diluted in passive lysis buffer (Promega, E1910) before the addition of furimazine (N1110) and measuring luminescence. Mass spectrometry Samples were prepared either by in gel digestion as

(16) Structural basis for RNA synthesis and inhibition of the orthobunyavirus polymeraseNature CommunicationsMay 15, 2026Jingjing Tang, Wenhua Kuang, Wei Chen, Hao Wu, Yong Wang, Jinyue Li, Zhiming Yuan, Han Xia, Haiyan Zhao, Zengqin Dengactivities of Nanoluciferase were measured using a Nano-Glo® Luciferase Assay System Kit (Promega, N1110) on a Varioskan™ LUX (Thermo Fisher Scientific). To examine the protein expression levels, BSR-T7/5

(17) Systemic Propagation of STING Signalling via Generation of Large Extracellular VesiclesJ Extracell VesiclesMay 9, 2026Jiae Lee, Annabel Vernon, Hyung Joon Park, Young V. Kwonplate with one well between samples to reduce cross talk. Nano‐Glo Luciferase Assay System (Promega, N1110) was used as directed to detect luminescence. Luminescence was measured using a SpectraMax i3x (Molecular Devices

(18) De novo and scaffold-based design of GDF15 binders for cancer cachexia diagnostics and therapeuticsExperimental & Molecular MedicineMay 8, 2026Jinsook Ahn, Ryeongeun Cho, Sohyun Kim, Dong Sun Lee, Ho Min KimGDF15 sample, 25 μl of PBS-diluted sensor protein, and 50 μl of Nano-Glo luciferase assay reagent (#N1110, Promega). After mixing serum-diluted GDF15 sample with PBS-diluted sensor protein, the plates were

(19) Selective positive allosteric modulation of β2AR using antibody fragments targeting the third intracellular loopNature CommunicationsMay 6, 2026Edgardo J. Sánchez Rivas, Fredrik Sadler, Mohammadamin Safdari, Nishaben M. Patel, Michael Ritt, Ning Ma, Sergio Branciamore, Nagarajan Vaidehi, Sivaraj Sivaramakrishnanconditions (+/- Vhh) was transferred to an opaque 96-well plate. 45 µl of Nano-Glo Substrate (Promega cat# N1110) diluted 1:50 in luminescence assay buffer was added to each well in the new plate. After tapping the

(20) A whole-animal phenotypic drug screen identifies suppressors of atherogenic lipoproteinsbioRxivMay 5, 2026Daniel J. Kelpsch, Liyun Zhang, James H. Thierer, Kobe Koren, Urmi Kumar, Yuki Lin, Monica R. Hensley, Mira Sohn, Jun O. Liu, Thomas Lectka, et al.light:dark cycle for 48 hours. At 5 dpf, a solution of anesthetic, fixative, and NanoGlo substrate (Promega N1110) was added to each well of each drug plate at a final concentration of 0.014% MESAB/4.6% paraformaldehyde

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Nano-Glo Luciferase Assay

Nano-Glo Luciferase Assay from Promega

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Nano-Glo Luciferase Assay from Promega

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