Nano-Glo Luciferase Assay N1110 from Promega

Product Specs
ItemNano-Glo Luciferase Assay
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberN1110
Quantity10ml

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Harnessing extracellular vesicles for stabilized and functional IL-10 delivery in macrophage immunomodulationExtracellular VesicleJune 29, 2026Najla A. Saleh, Matthew A. Gagea, Xheneta Vitija, Sadhana Kilangodi, Ahmed A. Zarea, Tomas Janovic, Jens C. Schmidt, Cheri X. Deng, Masamitsu Kanadaand NF-κB activity were analyzed by measuring bioluminescence signals. Furimazine (25 μM; Promega, N1110) was added for PalmReNL detection, and D-luciferin (300 μg/mL; Promega, E160A) was added for the NF-κB

(2) Extracellular ATP drives tryptophan metabolism and aryl hydrocarbon receptor activation to promote cellular senescenceJournal of Biological ChemistryJune 1, 2026Daniela Volonte, Steven J. Mullett, Stacy L. Gelhaus, Ferruccio Galbiatito quantify secreted NanoLuc luciferase using the Nano-Glo Luciferase Assay System (catalog number N1110, Promega) (97). Results were normalized based on protein concentration, as determined using the BCA

(3) Systemic Propagation of STING Signalling via Generation of Large Extracellular VesiclesJ Extracell VesiclesMay 9, 2026Jiae Lee, Annabel Vernon, Hyung Joon Park, Young V. Kwonplate with one well between samples to reduce cross talk. Nano‐Glo Luciferase Assay System (Promega, N1110) was used as directed to detect luminescence. Luminescence was measured using a SpectraMax i3x (Molecular Devices

(4) De novo and scaffold-based design of GDF15 binders for cancer cachexia diagnostics and therapeuticsExperimental & Molecular MedicineMay 8, 2026Jinsook Ahn, Ryeongeun Cho, Sohyun Kim, Dong Sun Lee, Ho Min KimGDF15 sample, 25 μl of PBS-diluted sensor protein, and 50 μl of Nano-Glo luciferase assay reagent (#N1110, Promega). After mixing serum-diluted GDF15 sample with PBS-diluted sensor protein, the plates were

(5) A whole-animal phenotypic drug screen identifies suppressors of atherogenic lipoproteinsbioRxivMay 5, 2026Daniel J. Kelpsch, Liyun Zhang, James H. Thierer, Kobe Koren, Urmi Kumar, Yuki Lin, Monica R. Hensley, Mira Sohn, Jun O. Liu, Thomas Lectka, et al.light:dark cycle for 48 hours. At 5 dpf, a solution of anesthetic, fixative, and NanoGlo substrate (Promega N1110) was added to each well of each drug plate at a final concentration of 0.014% MESAB/4.6% paraformaldehyde

(6) CK2 inhibition suppresses glial inflammation in models of neuroinflammation and neurodegenerationNature CommunicationsApril 22, 2026Ioana I. N. Da Silva, Desiree Ramirez, Sarah L. Parylak, Leslie A. Wallace, James K. Tucker, Joel M. Erberich, Rutvi Katariya, Aidan H. McDonald, Iryna S. Gallina, Ariana L. Tucker, et al.Samples were transferred to white 96-well plates and Nano-Glo Luciferase Assay master mix (Promega N1110) was added. Luminescence was measured after a 3-min incubation. Stabilization fold-change for each protein

(7) Expanded applications of bioluminescence microscopy with phasor analysisCell Reports MethodsApril 20, 2026Lila P. Halbers, Caroline K. Brennan, Lorenzo Scipioni, Giulia Tedeschi, Zachary R. Torrey, Kshitij Parag-Sharma, Bryan Labra, Christoph Gohlke, Antonio L. Amelio, Michelle A. Digman, et al.10035-04-8 EGTA,Sigma Aldrich,CAS Number: 67-42-5 STS,VWR,CAS Number: 62996-74-1 Furimazine,Promega,Catalog number: N1110 Puromycin,–,– Coelenterazine,GoldBio,Catalog Number:CZ2.5 CAS Number:55779-48-1 Catalase from bovine liver

(8) Microprotein Regulates G-quadruplex Driven RNA AggregationbioRxivApril 18, 2026Bikash R. Sahoo, Janakraj Bhattrai, Arnav Sharma, Ursula Jakob, James CA Bardwellfor 30 min in a 96-well format. Luciferase activity was measured using NanoGlo substrate (Promega, N1110) on a Tecan M1000 plate reader. Translation products were further analyzed by anti-FLAG immunoblotting

(9) Vacuum and Sonication Treatment Enable Efficient Transient Gene Expression in Various Monocot and Eudicot Plant SeedlingsACS Synthetic BiologyApril 17, 2026Eugene Li, Yuan Geng, Tuyako R. Khristoforova, Yunqing Wang, Jolie W. Jones, Kimberley T. Muchenje, Elisa Grillo, Hao Zhang, Robert J. Schmitz, Gozde S. Demirermin to pellet the cell debris. Luminescence was measured using the Nano-Glo Luciferase Assay System (N1110, Promega) according to the manufacturer’s instructions, with minor modifications. A 15 μL aliquot of

(10) Airway organoids reveal patterns of influenza A tropism and adaptation in wildlife speciesEmerg Microbes InfectApril 17, 2026Ferran Tarrés-Freixas, Gerardo Ceada, Francesc Català-Moll, Maria Casadellà, Sara Tolosa-Alarcón, Eloi Franco-Trepat, Rytis Boreika, Vanessa Almagro-Delgado, Noelia Carmona, Josep Estruch, et al.expression was assessed 72 h after SARS-CoV-2 inoculation using a Nano-Glo Luciferase Assay (Promega; #N1110) and measured with a Fluoroskan FL Plate Reader (Thermo Fisher). Supplementary Material

(11) CGM23 corresponds to a pan-coronavirus lipopeptide inhibitor potently inhibiting virion fusioniScienceApril 17, 2026Yusuke Matsui, Roland Schwarzer, Mauricio Montano, Ekram W. Helmy, Rahul K. Suryawanshi, Taha Y. Taha, Yan Wang, Yvonne Angel, Marc Adler, M. Flori Sassano, et al.DMEM was add per well followed by 100 μLs of Nano-Glo Luciferase substrate/buffer cocktail (Promega, N1110). The plates were incubated for 5 minutes at 37°C and 5 % CO2 then read on a GloMax Discover luminometer.

(12) Engineering of genetically encoded programmable calcium channel inhibitory bindersNat CommunApril 13, 2026Xiaoxuan Liu, Sher Ali, Tien-Hung Lan, Decheng Wang, Brendan McKee, Tatsuki Nonomura, Siyao Liu, Feng Zhao, Michael X. Zhu, Yun Huang, et al.96-well plates. Bioluminescence was initiated using the Nano-Glo® Luciferase Assay System (Promega, N1110), and emission signals were recorded on a Cytation 5 Cell Imaging Multi-Mode Reader (BioTek). Raw BRET

(13) A synthetic angiotensin II/ACE2-based hormone shunt controlling experimental hypertensionNature CommunicationsApril 11, 2026Gokberk Unal, Maysam Mansouri, Yu-Qing Xie, Christian Mueller, Ghislaine Charpin-El Hamri, Martin Fusseneggerin vitro nanoluciferase analysis, the Nano-Glo Luciferase Assay System (Promega, Wisconsin, USA, cat. No. N1110) was used according to the manufacturer’s protocol. RAS measurements (i) Cell-based stACE2 assay.

(14) Root development progression involves RALF peptide-mediated ROS signaling and pectin dynamics in riceNature CommunicationsApril 10, 2026Meiru Jia, Hanlei Yang, Bingyue Li, Zhao Xie, Shaoxue Cao, Leiting Pan, Baocai Zhang, Yihua ZhouDTT, 0.1 mM EDTA) for 1 h, the gel was developed using Nano-Glo Luciferase Assay Substrate (Promega, N1110) and was imaged using a Tanon 5200 chemiluminescent imager. The relative protein level was determined

(15) U2AF regulates the translation and localization of nuclear-encoded mitochondrial mRNAsMol CellApril 2, 2026Gloria R. Garcia, Murali Palangat, Josquin Moraly, Benjamin T. Donovan, Bixuan Wang, Ira Phadke, David Sturgill, Jiji Chen, Guofeng Zhang, Ronald J. Holewinski, et al.Concentrator Kit,Zymo,D4003 HiScribe SP6 RNA synthesis kit,NEB,E2070S Nano-Glo Luciferase Assay,Promega,N1110 MitoTracker Red CMXRos,Invitrogen,2351994 FoxP3 Staining Buffer Set,Thermo Fisher,00-5523-00 ImProm-II reverse transcription system

(16) A dual-reporter mouse for therapeutic discovery in Angelman syndromeJCI InsightMarch 9, 2026Hanna Vihma, Lucas M. James, Hannah C. Nourie, Audrey L. Smith, Siyuan Liang, Carlee A. Friar, Tasmai Vulli, Lei Xing, Dale O. Cowley, Alain C. Burette, et al.substrate per well, followed by luminescence measurement using the Nano-Glo Luciferase Assay System (N1110, Promega), according to the manufacturers’ protocols. Fluorescence and luminescence were measured on

(17) An innovative nasal nanovaccine against SARS-CoV-2 induces systemic and upper airway immunity controlling viral replicationnpj VaccinesMarch 4, 2026Roberta L. Pagni, Edecio Cunha-Neto, Yasmin da Silva Santos, Edilberto Postól, Raquel Elaine de Alencar, Ana Moretti, Jonnatan J. Santos, Tamires L. Silva, Isabela P. Daher, Marcos C. Knirsch, et al.Cell Culture Lysis reagent (#E1500, Promega), and luciferase activity was measured using the Nano-Glo system (#N1110, Promega) in a luminometer (GloMax® Navigator Microplate Luminometer). The 50% inhibitory concentration

(18) A Sensitive Reporter Mouse Model to Study Adipocyte‐Derived Extracellular Vesicles In VivoJournal of Extracellular VesiclesFebruary 19, 2026Didde Riisager Hansen, Rugivan Sabaratnam, Lasse Bach Steffensen, Per Svenningsenfluids, and cell‐conditioned medium was measured using the Nano‐Glo Luciferase Assay System (Promega, N1110). Luminescence was measured in Relative Light Units per second (RLU/s) using the Orion L Microplate

(19) Negative feedback regulation of STING signaling by TAX1BP1-directed GolgiphagyNature CommunicationsFebruary 11, 2026Sujit Suklabaidya, Suchitra Mohanty, Irene E. Reider, Jesse White, Dominic Colter, Sarah M. McCormick, Noula Shembade, Young Bong Choi, Christopher C. Norbury, Edward W. Harhajin triplicate and treated with 25 µl of Opti-MEM I medium supplemented with 1% furimazine (Promega; N1110) for 5 min as described previously35. Luminescence intensities were measured using a GloMax 96 microplate luminometer

(20) Enhancing Circular RNA Translation Efficiency Through Dual Internal Ribosome Entry SitesBiology (Basel)February 11, 2026Yawen Sun, Yimin Zhang, Weijie Chen, Ting Chen, Yunlong Zhang, Shanyu Zhang, Changrui Luroom temperature for 15 min. Nano-Glo® Luciferase Assay Reagent (Promega, Madison, WI, USA; Cat. No. N1110) was prepared according to the manufacturer’s instructions, and 100 µL was added to each well. The plates

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Nano-Glo Luciferase Assay

Nano-Glo Luciferase Assay from Promega

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Nano-Glo Luciferase Assay from Promega

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