Dual-Luciferase Reporter 1000 Assay System E1980 from Promega

Product Specs
ItemDual-Luciferase Reporter 1000 Assay System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE1980
ReactivityMammalian
SpeciesMammalian
Detection TargetLuciferase
Assay Time4 seconds
MethodLuciferase
Quantity1,000 assays
Sample TypeMammalian cell
Sensitivity< />^-18
Molecular Weight~70–76kDa

Add to Compare List

Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Autoantibodies neutralizing type I IFNs in 40% of patients with WNV encephalitis in seven new cohortsJ Hum ImmunMay 4, 2026Adrian Gervais, Francesca Trespidi, Alessandro Ferrari, Francesca Rovida, Astrid Marchal, Stefania Croce, Irene Cassaniti, Mattia Moratti, Jennifer L. Uhrlaub, David M. Florian, et al.containing 5% CO2. Finally, the cells were lysed by incubation with a lysis buffer (provided in ref. number E1980; Promega) for 20 min at room temperature, and luciferase levels were measured with the Dual-Luciferase Reporter 1000 assay system

(2) Osteoblastic sclerostin loop3-LRP4 interaction required by sclerostin to inhibit bone formationBone ResApril 24, 2026Luyao Wang, Xiaohui Tao, Hewen Jiang, Shijian Ding, Ning Zhang, Xin Yang, Shenghang Wang, Yihao Zhang, Nanxi Li, Haitian Li, et al.analysis. Luciferase assays were performed using the Dual-Luciferase Reporter Assay System (Promega, E1980) according to the manufacturer’s protocol.40,41 Lentivirus transduction and puromycin selection WT-Lrp4

(3) ADAMDEC1-driven CCL2-CCR2-PD-1 axis in esophageal squamous cell carcinoma: a dual threat of O-GlcNAcylation and m6A methylation in neoadjuvant therapy resistance and immune evasionCell Oncol (Dordr)April 7, 2026Jiwei Cheng, Haibo Ma, Xin Qian, Wenqun Xingsh-GLI2) were set. At 48 h post-transfection, a Dual Luciferase Reporter Assay Kit (Promega, Cat. No. E1980) measured luciferase and Renilla signals per the manufacturer’s instructions. Relative luciferase activity

(4) Paraspeckles Are Associated with the Activation and Nuclear Localization of Unphosphorylated miR-34aNon-Coding RNAMarch 31, 2026Graham H. Read, Kristen McGreevy, Hanny Issawi, Tiffany Yang, Cynthia Tsang, Ihsan A. Turk, Emily Rietdorf, Whitaker Cohn, David W. Salzman, Julian P. Whitelegge, et al.two-second read time and no delay according to the Promega DualGLO protocol (Promega, Madison, WI, USA #E1980). Relative miRNA activity was calculated according to manufacturer specifications. Briefly, raw luminescence

(5) Pathogenic variants in the cohesin loader subunit MAU2 underlie a distinct Cornelia de Lange Syndrome subtypeNat CommunMarch 30, 2026Ilaria Parenti, Alina Hesters, Marta Gil-Salvador, Laura Duffy, Deniz Kanber, Jasmin Beygo, Jennifer Kerkhof, Laura Steenpaß, Elsa Leitão, Julia Woestefeld, et al.Renilla luciferases was measured 24 h post-transfection with the Dual Luciferase Reporter Assay System (E1980, Promega) using the GloMax Discover System (Promega). All measurements were performed in triplicate

(6) Functional siRNA Delivery via Jet Nebulization: Proof-of-Concept IL-1ß Silencing in Macrophage-like THP-1 CellsInt J Mol SciMarch 23, 2026Duy Bao Tran Nguyen, Ahmed S. M. Ali, Dongwei Wu, Johanna Berg, Daniel C. Lauster, Jens Kurreck, Beatrice Tolksdorfpost-transfection by measuring relative luciferase activity using the Dual-Luciferase Reporter Assay System (E1980, Promega), according to the manufacturer’s instructions. 4.13. Western Blot Differentiated and LPS-stimulated

(7) SAMHD1 depletion restricts SARS-CoV-2 infection by suppressing HNF1-dependent ACE2 expression in lung epithelial cellsPLoS PathogMarch 19, 2026Pak-Hin Hinson Cheung, Pearl Chan, Hua Yang, Krisztina Ambrus, Shravya Honne, Baek Kim, Stanley Perlman, Li Wufollowing manufacturer’s instruction to quantify the resulting chemiluminescence (Promega, Cat. no. E1980). The cellular firefly luciferase 2 activities were calculated as follow: (RLUinfected per one microgram

(8) Functional characterization of the GWAS lead SNP rs888663 and effects of GDF15 SNPs on GDF15 levels in gestational hypertension and preeclampsiaMol Biol RepMarch 7, 2026Daniela A. Pereira, Julyane N. S. Kaihara, Luis Fernando P. Passeti, João Locke F. Araújo, Renan P. Souza, Ana C. Palei, Ricardo C. Cavalli, Chengyu Deng, Nadav Ahituv, Valeria C. Sandrim, et al.measured for Firefly and Renilla luciferase activity using the Dual-Luciferase Reporter Assay System (E1980, Promega) in a GloMax 96 microplate Dual Injector Luminometer (Promega) following the manufacturer’s

(9) Brucella Omp25 activates the unfolded protein response to promote intracellular proliferation and inflammationJournal of Biological ChemistryFebruary 26, 2026Jin-Ke Yang, Shuang Huang, Yuan-Pan Hou, Hui-Fei Yuan, Yue Wang, Mi Li, Li-Bo Cao, Tian Xia, Hong-Bing Shu, Xin Wurespectively. The Protein A+G Agarose (P2055, Beyotime), PMSF (P7626, Sigma), Dual-Specific Luciferase Assay Kit (E1980, Promega); SYBR Green supermix (Q312, Vazyme); HiScript II Select RT SuperMix for qPCR (R323, Vazyme);

(10) Foot-and-mouth disease virus VP4 interferes with host interferon response by targeting the nuclear translocation of interferon regulatory factor 3 (IRF3)Veterinary ResearchFebruary 3, 2026Ashan Subasinghe, N. A. Nadeeka Nethmini, Asela Weerawardhana, Nuwan Gamage, D. K. Haluwana, Jong-Soo Leeand luciferase reporter activity was measured via the dual-luciferase reporter assay system (Promega, E1980). Results were quantified compared with Renilla luciferase expressed independently (internal control). Immunoprecipitation Transfected

(11) The ASFV CD2v protein inhibits apoptosis by inducing proteasomal degradation of Bim EL via activation of the TPL2-MEK-ERK signaling pathwayJournal of VirologyFebruary 3, 2026Jianyu Zeng, Xin Zhang, Jingting Zhao, Qiang Li, Zhiyong Xiang, Fengyang Shi, Hua Wang, Wenlian Weng, Qiongqiong Zhou, Peng Gao, et al.purchased from Shandong Sparkjade Biotechnology Co., Ltd. The dual-luciferase reporter assay system kit (E1980) was purchased from Promega. pAP-1-Luc reporter vector (VT1587) was purchased from YouBio Biotech Co.,

(12) Inducible CD147 up-regulation boosts extended SARS-CoV-2 infection triggering severe COVID-19 independent of ACE2Signal Transduct Target TherFebruary 3, 2026Ke Wang, Peng Lin, Ruo Chen, Qiang Huang, Yizhen Zhao, Lei Zhang, Yongxiang Zhao, Liping Zhong, Ke Xu, Linlin Bao, et al.efficiency evaluated by detecting firefly fluorescence signal using Dual-Luciferase Reporter Assay System (E1980, Promega) according to the manufacturer’s protocols. AHR agonist 2 (HY-144339, MCE) and AHR antagonist 5

(13) Effector–host interactome map links type III secretion systems in healthy gut microbiomes to immune modulationNature MicrobiologyJanuary 26, 2026Veronika Young, Bushra Dohai, Hridi Halder, Jaime Fernandez-Macgregor, Niels S. van Heusden, Thomas C. A. Hitch, Benjamin Weller, Patrick Hyden, Deeya Saha, Daan K. J. Pieren, et al.SRP3177) at 24 h post transfection. Lysates were analysed using the dual-luciferase reporter kit (Promega, E1980) with a luminometer (Berthold Centro LB960 microplate reader, software: MikroWin 2010). NF-κB induction

(14) Functional variants at 1p36.23 confer risk of schizophrenia through modulating RERENature CommunicationsJanuary 24, 2026Yixing Liu, Junyang Wang, Hong Yang, Yifan Li, Changgai Mu, Xinglun Dang, Xinran Chen, Danyang Li, Mingna Li, Jiewei Liu, et al.L3000-015). Twenty-four hours post-transfection, the Dual-Luciferase® Reporter 1000 Assay System (Promega, E1980) was used to quantify luciferase activity in HEK-293T cells. For SH-SY5Y and U251 cells, luciferase

(15) Novel GS5 sericin mitigates UVA-induced photoaging by activating Nrf2 and inhibiting the JAK-STAT pathwayInternational Journal of Biological SciencesJanuary 14, 2026Haiqiong Guo, Yueting Sun, Wenyu Shi, Rui Huang, Qingxiu He, Ping Zhao, Qingyou XiaARE gene) were peptide-treated 8 h post-transfection. After 24 h, luciferase activity was quantified (E1980, Promega, Madison, WI, USA) using t-BHQ (HY-100489, MCE, Merced, CA, USA) as positive control. Firefly

(16) IRF4 haploinsufficiency in a multiplex family with Whipple’s diseaseJournal of Human ImmunityJanuary 5, 2026Sinem Ünal, Stéphanie Dublanc, Hailun Li, Jean-Philippe Vernhes, Vincent Meynier, Camille Soudée, Whipple’s consortium, Achille Aouba, Sylvain Audia, Nabil Bel Feki, et al.1X, Promega # E1910). Lysed cells were used for the ISRE assay with the dual-Luciferase kit (Promega #E1980), according to the manufacturer’s protocol. Signal intensity was determined with Envision plate reader

(17) Fuzheng Jiedu formula attenuates acute pneumonia by coordinated regulation of macrophage NLRP3 inflammasome and neutrophil NETsChin MedJanuary 4, 2026Kaixin Liu, Jin Yan, Fuyun Chi, Wenshuang Wang, Man Zhang, Yuanyuan Hou, Gang Baiand Renilla luciferase activities were measured using a luciferase reporter gene detection system (E1980; Promega, Wisconsin, USA), with luminescence captured by a multifunctional microplate reader (Tecan,

(18) Epromoters bind key stress-related transcription factors to regulate clusters of stress response genesThe EMBO JournalJanuary 3, 2026Juliette Malfait, Jing Wan, Himanshu Narayan Singh, Charbel Souaid, Gaëlle Farah, Junhua Su, Magali Torres, Iris Manosalva, Nathalie Sakakini, Cyril Esnault, et al.followed by a recovery step of 2 h in an incubator at 37 °C and 5% CO2. The Dual-Luciferase kit (Promega, E1980) was used to measure luciferase and Renilla luminescence following the manufacturer’s recommendations.

(19) T follicular helper cells transiently unlock a plasticity state in germinal centre B cells during the humoral immune responseNature Cell BiologyJanuary 1, 2026Laurianne Scourzic, Franco Izzo, Matt Teater, Alexander P. Polyzos, Lucretia Cucereavii, Christopher R. Chin, Antonin Papin, Hugo B. Pinto, Coraline Mlynarczyk, Ioanna Tsialta, et al.previously described124 was performed using the Dual-Luciferase Reporter 1000 Assay System (Promega #E1980). Briefly, 125,000 cells from each sample were spun down at 300g for 5 minutes. Cell pellets were then

(20) Prevalence and association of type I IFN autoantibodies with clinical outcomes in critically ill Brazilian COVID-19 patientsJ Hum ImmunDecember 15, 2025Graziela P Peduti, Isadora M Paiva, Adrian Gervais, Lorena V Andrade, Beatriz Vaconcelos, Pablo Rs Oliveira, Luydson R Vasconcelos, Ricardo Khouri, Thiago M Cunha, Carlos Df Souza, et al.luciferase levels were measured with the Dual-Luciferase Reporter 1000 assay system (reference number E1980; Promega) according to the manufacturer’s protocol. Luminescence intensity was measured with a FlexStation 3

Write a Review

(1)

2/2/2024Brilliant Experience With the Luciferase Reporter Assay System From Promega Parijat SarkarI used the dual luciferase assay to measure transcriptional activation using a Gal4 synthetic transcription assay. I co-transfected the GAL4 upstream activation sequence-driven firefly luciferase ... Read Review
(1)

1/7/2016Dual Luciferase Reporter, Promega Ganesh PusapatiPrimary cilia are tiny microtubule based structures that project from the cell surface. These structures serve as the signaling hubs for the Sonic hedgehog signaling. The goal of this experiment is ... Read Review
(1)

11/3/2015Lucifersae Assay Somasundaram RaghavanReporter systems are generally used to study eukaryotic gene expression. These dual reporters are generally used for experimental accuracy. Basically here we can express as well as measure the two ... Read Review

Dual-Luciferase Reporter Assay System From Promega
The luciferase assay is one of the most well-known and convenient reporter assays. In particular, ... Read More
Dual-Luciferase® Reporter Assay System from Promega
Genetic reporters such as firefly luciferase are commonly used in molecular and cellular biology ... Read More

Dual-Luciferase Reporter 1000 Assay System

Dual-Luciferase Reporter 1000 Assay System from Promega

Description

Promega

Citations (420)

Reviews

Product Reviews

Dual-Luciferase Reporter Assay System From Promega

Dual-Luciferase® Reporter Assay System from Promega

Dual-Luciferase Reporter 1000 Assay System from Promega

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map