Promega
Nano-Glo® Luciferase Assay System
N1120
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Our lab uses HIV molecular clones that express Nano luciferase reporter. This reporter is very stable and allows quatification of low HIV infection, because it provides a very sensitive reading. We infect cells in vitro, or perform in vivo experiments where we infect our mice with these viruses. As a read out, we test cell lystaes for their luciferase activity by using this kit.
Renilla, firefly, and nano luciferase activity measurement
HIV infected cell lysates
Cells are lysed in 1x lysis buffer, vortexed well, stored in -80 degrees for at least one hour, thawed, then read in presence of substrate.
Vortex cells well
Very sensitive assay.
N/A
Work very well
None
I highly recommend this kit. Very sensitive assay.
Product Name: Nano-Glo Luciferase AssaySupplier Page