Bright-Glo Luciferase Assay System E2620 from Promega

Product Specs
ItemBright-Glo Luciferase Assay System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE2620
ReactivityMammalian
Detection TargetLuciferase
SpeciesMammalian
Quantity100ml
Sample TypeMammalian cells
MethodLuciferase
SensitivityUp to tenfold more light intensity than other homogeneous luciferase assay reagents results in increased sensitivity.

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Sialic acid cis-ligand dynamics modulate Siglec-7 and -9 function and affect Siglec-7/9 co-blockade to potentiate natural killer cell anti-tumor activityOncoimmunologyDecember 31, 2026Jesper van Eck van der Sluijs, Anne H. C. Valk, Eline J. H. van Houtum, Esther D. Kers-Rebel, Maaike Looman, Willemijn Hobo, Anna E. Marneth, Harry Dolstra, Lune van den Boogaard, Christian Büll, et al.and 5% CO2, followed by luminescence measurement using Bright-Glo™ Luciferase Assay System (Promega, E2620) according to the manufacturer’s protocol on a Victor3 Multilabel Plate Reader (Perkin Elmer). Western blot

(2) Induced-fit anti-GFRAL antibody ameliorates cancer cachexia and attenuates sympathetic outflow in tumor-bearing miceSci AdvOctober 30, 2026Sohyun Kim, Min Hee Lee, Jinsook Ahn, Sang-Hyeon Ju, Dong Sun Lee, Seohyun Choi, Kwang Wook Min, Minho Shong, Ho Min Kimmeasured by determining absorbance at 560 nm using the Bright-Glo Luciferase Assay System (Promega, #E2620) and a SpectraMax iD5 microplate reader. Data for IC50 determination were normalized to the GDF15-only

(3) Regulatable in vivo gene expression via adaptamersMol CellOctober 20, 2026Jack Bryant, Laura Herron, Yesh Doctor, Finn McRae, Jocelyn Wang, Theodore Hirsch, Andrew Portell, Satheesh Kumar, Prashant MaliSystem,Promega,N1120 Dextrose,MilliporeSigma,G7021-1KG "Neutral Buffered Formalin, 10%",MilliporeSigma,HT501128-4L Bright-Glo™ Luciferase Assay System,Promega,E2610 RiboGreen®,ThermoFisher,R11491 Purified ADAR1 p150 protein,BPS Bioscience,100472-1 HiScribe® T7 High Yield RNA Synthesis Kit

(4) Functional redundancy, complementation, and cross-talk between CH25h and IFITM3 in infection and in IFITM3 trafficking and secretionJournal of VirologyOctober 20, 2026Yuxin Song, Tingting Xia, Charlène Martin, Gaetan Despres, Julien Burlaud-Gaillard, Antonin Lamaziere, Philippe Roingeard, Andrea Cimarelliwas quantified after luciferase analysis on cell lysates using the BrightGlow Lysis Reagent (Promega E2620) and the Tecan Spark Luminometer. Infectious virus yields under various conditions were consistently

(5) Direct detection and quantification of HIV particles and their envelope trimers bound by combinations of broadly neutralizing antibodiesiScienceOctober 16, 2026Souradip Dasgupta, Aswin T. Srivatsav, Sameer Kumar, George K. Lewis, Anthony L. DeVico, Krishanu Raycommercial assays,Critical commercial assays ,, Bright-Glo™ Luciferase Assay System,Promega,Catalog #E2610 NuPAGE™ 4–12% Bis-Tris Protein Gels,Thermo Fisher Scientific,Catalog # NP0322PK2 Whatman® Optitran®

(6) Cucurbitacin B suppresses GBM progression by inducing GADD45α-Dependent PCNA downregulation and DNA replication arrestFront PharmacolOctober 14, 2026Yonghua Xue, Bin Wangnon-transduced cells. Luciferase activity was quantified using the Bright-Glo Luciferase Assay System (Promega, E2620) and measured on a Varioskan LUX multimode microplate reader (Thermo Fisher Scientific). Cell populations

(7) Cancers modulate processing and presentation of p53 neoantigens to evade T cell detectionImmunityOctober 13, 2026Koji Haratani, Bruce Reinhold, Jonathan S. Duke-Cohan, Caroline G. Fahey, Kemin Tan, Robert J. Mallis, Alexander Gusev, Kenneth L. Kehl, Jia Luo, Allyson Karmazyn, et al.according to the manufacturer’s instructions. In case of T2 cells, the Bright-Glo Luciferase Assay System (E2610, Promega) was used. Luminescence data were obtained two times serially from the same plates to minimize

(8) Hippo signaling regulates spontaneous human trophoblast syncytialization in three-dimensional culturesMolecular Human ReproductionOctober 7, 2026Michael Zheng, Lin Zhao, Richard Xi, Jahdiel DeNobrega, Mary Tammo, Stephen J Renaud, Peeyush K Lalaof DMSO in complete TS media for 24 h. Cells were lysed with the Bright-Glo Luciferase assay system (E2610, Promega, Madison, WI, USA), with luciferase activity measured using a VICTOR3V luminescence microplate

(9) Development and Characterization of Pseudotype-based Microneutralization Assay to Assess Functionality of Anti-rabies Protein G AntibodiesF1000ResOctober 6, 2026Antonelli R#, Vezzani G#, Guerrini N, Giovagnoli V, Dapporto F, Iturriza M, Loomis R, Montomoli E, Rossi O, Molesti E, et al.72-hour incubation at 37°C with 5% CO 2, 50 μL of Bright-Glo™ Luciferase Assay Reagent (Promega, Cat. No. E2620) was added per well. The plates were shaken for 5 minutes at 400 RPM using a microplate shaker (Fisherbrand,

(10) Novel African Rhinolophus bat ACE2 sequences reveal the determinants of Afro-Eurasian sarbecovirus entryJournal of VirologySeptember 22, 2026Yueying Zhang, Shigeru Fujita, Masahiro Kajihara, Katendi Changula, Bernard Mudenda Hang'ombe, Shusuke Kawakubo, Spyros Lytras, Jumpei Ito, Akinori Kanai, Yutaka Suzuki, et al.respectively, and infected cells were lysed with a Bright-Glo Luciferase Assay System (Promega, Cat #E2620) 2 days post-infection. The luminescent signal was measured using a GloMax Explorer Multimode Microplate

(11) Intragenic transcription from defective HIV proviruses triggers interferon responses in myeloid cellsJournal of VirologySeptember 22, 2026Jonathan M. Kilroy, Aparna A. Deokar, Samantha Patalano, Juan I. Fuxman Bass, Manish Sagar, Suryaram Gummuluru, Andrew J. Hendersonmedia/supernatant was aspirated from these plates, and 30 µL of BrightGlo reagent (Promega Catalog No. E2610) was added to lyse the cells and measure luciferase activity. A standard curve was generated by adding

(12) Intravenously deliverable oncolytic virus VET3-TGI favorably alters the tumor microenvironmentJournal for immunotherapy of cancerSeptember 21, 2026Zhang M, Barrett K, Carter C, Sampath P, Acosta-Barreto G, Audino S, Moongdee J, Bouchard R, Dunstane D, Matteo N, et al.final concentrations shown. Signal was developed using a Bright-Glo luciferase assay kit (Promega, E2620) and measured using a Spark 10M plate reader. Detection of CXCR3 expression on infected peripheral blood mononuclear cells

(13) A NanoLuc-Based Protease Biosensor for Highly Sensitive Detection of Intracellular Protease Activity: Applications to Apoptosis and Coronavirus InfectionBio ProtocSeptember 20, 2026Masashi Arakawa, Eiji Morita(Promega, catalog number: N1120) 2. Bright-GloTM luciferase assay system (Promega, catalog number: E2620) Lysis buffer components 1. NaCl (Nacalai Tesque, catalog number: 31320-05) 2. Tris (Tris[hydroxymethyl]aminomethane)

(14) Identification of Galectin-9 (Gal-9) as a B7-H4 binding partner and characterization of their glycosylation-dependent interaction that modulates T cell signaling within a multi-ligand/receptor networkPLoS OneSeptember 18, 2026Ravear Zhiqiang Wang, Fang Yang, Jianhua Suiwas quantified by measuring luminescence using the Bright-Glo™ Luciferase Assay System Kit (Promega, E2620). MC38-mB7-H4 stable cells were established by transducing MC38 cells with lentiviruses expressing the

(15) Convergent use of aminopeptidase N receptor by hedgehog merbecovirusesNature MicrobiologySeptember 16, 2026Peng Liu, Qianhui Zhu, Jingyi Liu, Zhixing Ma, Guocui Mou, Yuanling Yu, Chen Liu, Chengbao Ma, Junyu Si, Xiao Yang, et al.luciferase activity was measured using the Bright-Glo Luciferase Assay System (Promega, catalogue no. E2620) on a Thermo Varioskan LUX or GloMax 20/20 Luminometer (Promega). For pseudovirus entry assays in primary

(16) Suidae iPSC-derived macrophages as models for investigating susceptibility and resilience to African swine fever virusStem Cell ReportsSeptember 8, 2026Tom M. Watson, Lynnette C. Goatley, Stephen Meek, Lel Eory, Sylvia Kohler, Nicholas Berkley, Sofia Sternberg, Melany Jackson, Amy Findlay, Isabella Hoskins, et al.(Promega #E2661) and transferred to white 96 well plates. An equal volume of Bright-Glo reagent (Promega, #E2610) was added and incubated for 5 min. The plates were read on a Promega GloMax Discovery microplate reader,

(17) Microfluidic-based rapid generation of chimeric antigen receptor T cells improves low-multiplicity-of-infection transduction efficiency while preserving functional activityJ Transl MedSeptember 5, 2026Ying Jiang, Guidong Zhu, Haiyan Zhang, Dongqi TangResidual viable target-cell signal was quantified using the Bright-Glo Luciferase Assay System (Promega; E2610) on a microplate luminometer (Molecular Devices, San Jose, CA). Specific lysis was calculated as 1 -

(18) Emergence of neutralizing RBD antibodies following Omicron infection with limited activity against ancestral SARS-CoV-2iScienceAugust 21, 2026Michael S. Piepenbrink, Yao Ma, Simran Panjwani, Alexandra R. Blake, Allison M. Bell, James L. Kizziah, Sara H. Mahmoud, Gregory C. Ippolito, Nathan B. Erdmann, Paul A. Goepfert, et al.(Fc) capture kit,Cytival Life Sciences,Cat#BR100839 Bright-Glo™ Luciferase Assay System,Promega,Cat#: E2620 ,, Deposited data,Deposited data,Deposited data ,, 1332D4-S(KP3.1.1) Structure,This study,PDBID

(19) SARS-CoV-2 Neutralization Breadth Is Shaped by Variant Exposure and Immune HistoryJ Med VirolAugust 13, 2026Greta Vizgirda, Alexander P. Underwood, Ulrik Fahnøe, Christina Sølund, Signe Lysemose Villadsen, Andrea Galli, Nina Weis, Jens Bukhquantified by measuring luminescence using the Bright‐Glo Luciferase Assay System (Promega; Cat. No. E2610) and the FLUOstar Omega microplate reader (BMG Labtech). Infection was considered positive when luminescence

(20) Directed evolution of a stem-helix-targeting antibody enables MERS-CoV cross-neutralization through enhanced binding affinityPLoS PathogAugust 7, 2026Panpan Zhou, Meng Yuan, Yuexiu Zhang, Oliver Limbo, Ge Song, Fangzhu Zhao, Hejun Liu, Wan-Ting He, Tazio Capozzola, Sean Callaghan, et al.well. After 48h of incubation, luciferase activity was measured by BrightGlo substrate (Promega Cat# E2620) according to the manufacturer’s instructions. Fifty percent maximal inhibitory concentrations (IC50s)

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Bright-Glo Luciferase Assay System

Bright-Glo Luciferase Assay System from Promega

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Promega

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Bright-Glo™ Luciferase Assay From Promega

Bright-Glo™ Luciferase Assay System From Promega

Bright-Glo Luciferase Assay System from Promega

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