Bright-Glo Luciferase Assay System E2620 from Promega

Product Specs
ItemBright-Glo Luciferase Assay System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE2620
ReactivityMammalian
Detection TargetLuciferase
SpeciesMammalian
Quantity100ml
Sample TypeMammalian cells
MethodLuciferase
SensitivityUp to tenfold more light intensity than other homogeneous luciferase assay reagents results in increased sensitivity.

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Sialic acid cis-ligand dynamics modulate Siglec-7 and -9 function and affect Siglec-7/9 co-blockade to potentiate natural killer cell anti-tumor activityOncoimmunologyDecember 31, 2026Jesper van Eck van der Sluijs, Anne H. C. Valk, Eline J. H. van Houtum, Esther D. Kers-Rebel, Maaike Looman, Willemijn Hobo, Anna E. Marneth, Harry Dolstra, Lune van den Boogaard, Christian Büll, et al.and 5% CO2, followed by luminescence measurement using Bright-Glo™ Luciferase Assay System (Promega, E2620) according to the manufacturer’s protocol on a Victor3 Multilabel Plate Reader (Perkin Elmer). Western blot

(2) Intranasal Replicating Adenovirus type 4-Vectored SARS-CoV-2 Vaccines Induce Durable and Efficacious Responses in Preclinical TestingiScienceApril 17, 2026Breanna Kim, Rachel Roenicke, Peyton M. Roeder, Isabel Steinberg, Andy Patamawenu, Mitra Harrison, Freya van’t Veer, Emma Koory, Jane Xie, Tulley Shofner, et al.Ltd,Cat#067020 AdEasy Viral Titer Kit,Agilent Technologies,Cat#972500 Bright Glo Luciferase Assay System,Promega,Cat#E2610 Moderna mRNA −1273 vaccine,Moderna US Inc,Cat#80777011096 Luminex MagPlex Magnetic Microspheres,Diasorin,https://us.diasorin.com/en/luminex/products-and-services/microspheres/magplex-microspheres ZR BAC DNA Miniprep Kit

(3) Thymelaea hirsuta (L.) Endl. extract attenuates NLRP3 inflammasome activation via modulation of ATPase activityFront PharmacolApril 15, 2026Seongjong Lee, Seoyeon Jang, Hangyeol Lee, Ali Khorchani, Abdelhamid Khaldi, Mi Jin Lee, Dong Keun Yi, Soo-Yong Kim, Sangho Choi, Hyungseok Seo, et al.DAPI (ab104139; Abcam, United Kingdom). For reporter assays, the Bright-Glo™ Luciferase Assay System (E2610), pGL4.32 [luc2P/NF-κB-RE/Hygro] vector (E8491), and the ADP-Glo™ Max Assay (V7001) were purchased from

(4) Intragenic Transcription from Defective HIV Proviruses Triggers Interferon Responses in Myeloid CellsbioRxivApril 7, 2026Jonathan Kilroy, Aparna Deokar, Samantha Patalano, Juan Fuxman Bass, Manish Sagar, Suryaram Gummuluru, Andrew J. HendersonMedia/supernatant was aspirated from these plates and 30 μL of BrightGlo reagent (Promega Catalog No. E2610) were added to lyse the cells and measure luciferase activity. A standard curve was generated by adding

(5) Receptor-guided AAV Tropism Engineering via MATCHbioRxivApril 1, 2026Nolan Graham, Satheesh Kumar, Joseph Rainaldi, Seoin Yang, Andrew Portell, Billy Santoso, Prashant MaliCK04-01) before luciferase activity was assessed using the Bright-Glo™ Luciferase Assay System (Promega, E2610) according to manufacturer’s protocols. Luciferase signal was then normalized to relative cell viability. Human

(6) TNF inhibits SARS-CoV-2 induced cell-cell fusion through activating the SDC4-RhoA signaling to promote actin bundles formationCell InsightApril 1, 2026Dong Duan, Xu Zheng, Yanqiu Zhou, Mengmeng Cui, Yunyi Li, Xiaoxian Cui, Yuying Yang, Min Chen, Huanyu Wu, Xin Chen, et al.EDTA-free protease inhibitor cocktail (Roche)). Lysates were mixed with Bright-Glo luciferase substrate (E2610, Promega), and relative luminescence units (RLU, 1-min read) were measured using a Synergy H1 plate

(7) Timosaponin AIII enhances CAR-T cell potency and prevents relapse through impairing CAR-TregsNature CommunicationsMarch 31, 2026Mingqi Hou, Wenjun Zhang, Ziping Qi, Guiming Li, Husheng Mei, Shuang Qi, Rui Jin, Yuedong Zhao, Xiaochen Tang, Bing Xiu, et al.Cell Viability Assay) was obtained from Vazyme (DD1101-02). The Bright-Glo™ Assay Reagent was obtained from Promega (E2620). The immobilon western HRP substrate was purchased from Millipore (WBKLS0500). The LANCETM cAMP 384

(8) Targeting oncogenic NTSR1 with liensinine reprograms Gq-mediated signaling to suppress lung adenocarcinomaChin MedMarch 25, 2026Yongfu Wang, Wei Liu, Pengzhuo Tao, Changmin Liu, Yizhen Yuan, Yajing Xue, Hanting Yang, Xiongfeng Liu, Xinyi Zhou, Shilin Chen, et al.After an 18-h incubation at 37 °C, 50 μL/well of diluted Bright-Glo Luciferase Assay reagent (Promega, E2610) was added and luminescence was measured on a plate luminometer immediately. In-cell ELISA for detection

(9) Engineering NK and T cells with metabolite-sensing receptors to target solid tumorsNature ImmunologyMarch 23, 2026Young-Min Kim, Min K. Tsai, Chang Sun, Olivia Laveroni, Reece Villarin Akana, Kristen Frombach, Livnat Jerbyeach well and incubated at 37 °C with 5% CO2 for 24 h. The next day, Bright-Glo solution (Promega, E2610) was added and incubated for 5 min at room temperature. Luminescence was measured using a Tecan Infinite

(10) Regulation of MRGPRX2-mediated mast cell function by competence-stimulating peptide 1 and pro-adrenomedullin peptideFrontiers in ImmunologyMarch 17, 2026Aetas Amponnawarat, Sangita Sutradhar, Chalatip Chompunud Na Ayudhya, Maram Bawazir, Ronit Sagi-Eisenberg, Hydar AliCatalog #ab104139) were purchased from Abcam. Bright-Glo Luciferase was purchased from Promega (Catalog #E2620). Dyngo-4a and Cycloheximide were obtained from Cayman Chemical (Catalog #29479 and #14126). Phycoerythrin

(11) Regulation of MRGPRX2-mediated mast cell function by competence-stimulating peptide 1 and pro-adrenomedullin peptideFront ImmunolMarch 17, 2026Aetas Amponnawarat, Sangita Sutradhar, Chalatip Chompunud Na Ayudhya, Maram Bawazir, Ronit Sagi-Eisenberg, Hydar AliCatalog #ab104139) were purchased from Abcam. Bright-Glo Luciferase was purchased from Promega (Catalog #E2620). Dyngo-4a and Cycloheximide were obtained from Cayman Chemical (Catalog #29479 and #14126). Phycoerythrin

(12) Epithelial sensing of vitamin A shapes intestinal antimicrobial defensebioRxivMarch 13, 2026Gabriella Quinn, Daniel C. Propheter, Kartik Kulkarni, Matthew Johnson, Gonçalo Vale, Jeffrey G. McDonald, Ann Johnson, Brian Hassell, Cassie L. Behrendt, Nikhil V. Munshi, et al.for Transcription Factors,Diagenode,Ref. C01010055 BrightGlo Luciferase Assay System,Promega,Ref. E2610 β-Galactosidase Enzyme Assay System,Promega,Ref. E2000 Deposited data,Deposited data,Deposited data scRNAseq

(13) Protease-Activated Receptor 1 as an Endogenous Model of Peptidergic Gαq-Gα12-Biased G Protein SignalingbioRxivMarch 5, 2026Braden S. Fallon, Robert A. Campbell, Justin G. Englisha PHERAstar FSX (BMG Labtech, RRID:SCR_027001) using the Bright-Glo Luciferase Assay (Promega, Cat# E2610). Bright-Glo reagent was diluted in an equal volume of assay buffer (20 mM HEPES in HBSS), and 10 μL

(14) Autologous neutralizing antibodies and polyfunctional T cells contribute to long-term HIV-1 post-intervention controlNature ImmunologyMarch 3, 2026Katie Fisher, Mauro A. Garcia, Giacomo S. Frattari, Chloé Naasz, Junlin Zhuo, Miriam Rosás-Umbert, Lisa L. Dietz, Anna Karina Juhl, Emma Falling Iversen, Rikke Olesen, et al.experiment). Viral infection was measured by luciferase production with Bright-Glo Reagent (Promega, E2610) 48 h later. For each pseudovirus, the percentage of infection (fu) was calculated as a fraction of

(15) CREB suppresses PGRP-SC2 to drive age-related immune senescence and gut dysbiosis in DrosophilaCell Death DiscoveryFebruary 24, 2026Saifei Wang, Bohan Qi, Peng Ma, Yao Zhang, Youjie Yin, Shuxin Chen, Hansong Dengassays CRE luciferase activity was measured using the Bright-Glo™ Luciferase Assay kit (Promega, Cat# E2610) according to the manufacture instructions. In brief, lysates from whole flies (n = 5) or guts from

(16) Dynamic estimation of metabolic state during CAR T cell productionCell Reports MethodsFebruary 23, 2026N. Suhas Jagannathan, Wei-Xiang Sin, Denise Bei Lin Teo, Faris Kairi, Yen Hoon Luah, Francesca Lorraine Wei Inng Lim, Michaela Su-Fern Seng, Shui Yen Soh, Yie Hou Lee, Lisa Tucker-Kellogg, et al.Dynabeads Human T-Expander CD3/CD28,Thermo Fisher Scientific,Cat# 11141D Bright-Glo™ Luciferase Assay System,Promega,Cat# E2610 ,, Experimental models: Cell lines,Experimental models: Cell lines,Experimental models: Cell lines ,, Lenti-X

(17) A 3D multi-compartment assembloid to study combined immune cell infiltration and cytotoxicityCell Reports MethodsFebruary 23, 2026Eban A. Hanna, Ashleigh J. Crawford, Wenxuan Du, Adrian Johnston, David Schell, Zeqi Wan, Ting-Hsi Chen, Fan Wu, Kehan Ren, Yeongseo Lim, et al.the supernatant was removed from each well, and 100uL of Bright-Glo luciferase assay reagent (Promega E2620) was added according to the manufacturer’s instructions. The plate was covered with foil to prevent

(18) Neutralization and ADCC reveal divergent spike-subdomain targeting across SARS-CoV-2 vaccine platforms in an African cohortiScienceFebruary 20, 2026Gerald Kevin Oluka, Joseph Ssebwana Katende, Laban Kato, Violet Ankunda, Jackson Sembera, Peter Ejou, Geoffrey Odoch, Angella Namuyanja, Pontiano Kaleebu, Jennifer Serwangacommercial assays ,, NK Isolation Reagent Kit,Milteny,130-092-657 Bright-Glo luciferase kit,Promega,Cat#: E2610 Nickel-Nitrilotriacetic acid (Ni-NTA),Qiagen,Cat# 30410 ,, Experimental models: Cell lines,Experimental models

(19) Targeting NPY5R—A Member of the NPY Receptor Family: Pharmacological and Transcriptomic Mechanisms of the Euphorbia Factor L2 Against Lung AdenocarcinomaPharmaceuticals (Basel)February 15, 2026Pengzhuo Tao, Wei Liu, Yongfu Wang, Yajing Xue, Changmin Liu, Yizhen Yuan, Kim Fey Leu, Shilin Chen, Chi Songwere treated for 18 h, and 50 μL of Bright-Glo Luciferase Assay Substrate (Promega, Madison, WI, USA, E2610) was then added to each well. Plates were thoroughly mixed, and luminescence was determined immediately

(20) Accessory microRNA byproducts expand RNA interference via microprocessor-mediated cleavage activationSci AdvFebruary 13, 2026Debora Mazzetti, Michal O. Nowicki, Himanshu Soni, Joshua D. Bernstock, Maya Groff, Luisa Esposito, Diego A. Hernandez, Lucia Altucci, Anna Krichevsky, Geoffrey Fell, et al.legend. Luciferase activity was detected using the Bright-Glo Luciferase Assay System (catalog no. E2610, Promega). Induction of NF-κB was obtained by the addition of TNF-α (10 ng/ml) to the culture medium. Western blot

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Bright-Glo Luciferase Assay System

Bright-Glo Luciferase Assay System from Promega

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Promega

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Bright-Glo™ Luciferase Assay From Promega

Bright-Glo™ Luciferase Assay System From Promega

Bright-Glo Luciferase Assay System from Promega

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