Bright-Glo Luciferase Assay System E2620 from Promega

Product Specs
ItemBright-Glo Luciferase Assay System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE2620
Quantity100ml
ReactivityMammalian
Sample TypeMammalian cells
MethodLuciferase
SensitivityUp to tenfold more light intensity than other homogeneous luciferase assay reagents results in increased sensitivity.
Detection TargetLuciferase
SpeciesMammalian

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Sialic acid cis-ligand dynamics modulate Siglec-7 and -9 function and affect Siglec-7/9 co-blockade to potentiate natural killer cell anti-tumor activityOncoimmunologyDecember 31, 2026Jesper van Eck van der Sluijs, Anne H. C. Valk, Eline J. H. van Houtum, Esther D. Kers-Rebel, Maaike Looman, Willemijn Hobo, Anna E. Marneth, Harry Dolstra, Lune van den Boogaard, Christian Büll, et al.and 5% CO2, followed by luminescence measurement using Bright-Glo™ Luciferase Assay System (Promega, E2620) according to the manufacturer’s protocol on a Victor3 Multilabel Plate Reader (Perkin Elmer). Western blot

(2) DNA-based delivery of incretin receptor agonists using MYO Technology leads to durable weight loss in a diet-induced obesity modelMol Ther Nucleic AcidsJune 16, 2026Linda Sasset, Andrew D. Cameron, Carleigh Sussman, Luisa Rubinelli, Debnath Maji, Robert Miller, Andy Thompson, Delcora Campbell, Melanie R. Walker, Marek M. Drozdz, et al.IRAs. After 3 h of incubation, cells were lysed with Bright-Glo Luciferase Assay System (Promega, cat# E2610) according to the manufacturer’s protocol, and the luminescence was measured using GloMax Navigator

(3) Algorithm-Optimized H5 Influenza mRNA Vaccine Induces Broad Immune ResponsesInt J Mol SciMay 19, 2026Liangliang Wang, Zhengda Peng, Chenchen He, Jie Zhang, Pengju Yu, Weijin Huang, Youchun Wang, Chenyan Zhaoh. After removing the supernatant, luciferase activity was measured using a luciferase assay system (E2620, Promega) with 2 min of shaking. Luminescence was quantified using a PerkinElmer EnSight(Waltham, MA,

(4) Distinct cytokines regulate gene expression and anti-tumor activity in regenerated CD8+ T cells from induced pluripotent stem cellsiScienceMay 15, 2026Kenta Kondo, Nobuhito Nitta, Koji Terada, Yusuke Sano, Mina Kumode, Seiji Nagano, Kyoko Masuda, Daisuke Okuzaki, Toru Miyake, Masaji Tani, et al.hours. The luciferase activity was measured using the Bright-Glo™ Luciferase Assay System (Promega, E2610) and expressed as relative light units (RLU). Target cells alone were cultured alone to measure the

(5) Genomic and phenotypic comparisons reveal lineage-specific traits of the probiotic Saccharomyces boulardiiiScienceMay 15, 2026Hannah Elena Duffey, Karl Alex Hedin, Hitesh P. Gelli, Troels Holger Vaaben, Morten Otto Alexander Sommerassays ,, QUANTI-Luc reagent,InvivoGen,Cat. No. rep-qlc4r2 Bright-Glo Luciferase assay,Promega,Cat. No. E2610 IL-8 ELISA kit,R&D Systems,Cat. No. DY208 TNFα ELISA kit,Abcam,Cat. No. ab181421 Yeast Live-or-Dye™

(6) Protocol for lentiviral generation and transduction of human-derived endometrial epithelial organoids to study uterine function and pathologiesSTAR ProtocMay 7, 2026Sydney E. Parks, Diana Monsivaisassays,Critical commercial assays,Critical commercial assays ,, Bright-Glo Luciferase Assay System,Promega,Cat# E2610 Direct-zol RNA Miniprep,Genesee,Cat# 11-331 ,, Experimental models: Cell lines,Experimental models:

(7) Multifunctional ACE2-nanobody fusion design for pan-specific neutralization and cardiovascular protection in SARS coronavirus infectionAntimicrobial Agents and ChemotherapyMay 6, 2026Traian Sulea, Matthew Stuible, Maria Moreno, Alex Pelletier, Martin A. Rossotti, Yuneivy Cepero-Donates, Jacqueline Slinn, Patrick Salois, Anh Tran, Melissa Hewitt, et al.was then measured by luminescence. Bright-Glo luciferase reagent (Promega, Madison, WI, USA, Cat no. E2620) was added to wells for 2 min before reading with a PerkinElmer Envision instrument. Half-maximal inhibitory

(8) Intranasal Replicating Adenovirus type 4-Vectored SARS-CoV-2 Vaccines Induce Durable and Efficacious Responses in Preclinical TestingiScienceApril 17, 2026Breanna Kim, Rachel Roenicke, Peyton M. Roeder, Isabel Steinberg, Andy Patamawenu, Mitra Harrison, Freya van’t Veer, Emma Koory, Jane Xie, Tulley Shofner, et al.Ltd,Cat#067020 AdEasy Viral Titer Kit,Agilent Technologies,Cat#972500 Bright Glo Luciferase Assay System,Promega,Cat#E2610 Moderna mRNA −1273 vaccine,Moderna US Inc,Cat#80777011096 Luminex MagPlex Magnetic Microspheres,Diasorin,https://us.diasorin.com/en/luminex/products-and-services/microspheres/magplex-microspheres ZR BAC DNA Miniprep Kit

(9) Thymelaea hirsuta (L.) Endl. extract attenuates NLRP3 inflammasome activation via modulation of ATPase activityFront PharmacolApril 15, 2026Seongjong Lee, Seoyeon Jang, Hangyeol Lee, Ali Khorchani, Abdelhamid Khaldi, Mi Jin Lee, Dong Keun Yi, Soo-Yong Kim, Sangho Choi, Hyungseok Seo, et al.DAPI (ab104139; Abcam, United Kingdom). For reporter assays, the Bright-Glo™ Luciferase Assay System (E2610), pGL4.32 [luc2P/NF-κB-RE/Hygro] vector (E8491), and the ADP-Glo™ Max Assay (V7001) were purchased from

(10) ACE2 utilization of HKU25 clade MERS-related coronaviruses with broad geographic distributionbioRxivApril 12, 2026Chen Liu, Young-Jun Park, Cheng-Bao Ma, Cameron Stuart, Risako Gen, Yu-Cheng Sun, Xiao Yang, Mei-Yi Lin, Qing Xiong, Jun-Yu Si, et al.(Relative light units, RLU) was measured at 18 hpi using the Bright-Glo Luciferase Assay Kit (Promega, E2620) and detected with a GloMax 20/20 Luminometer (Promega) or Varioskan LUX Multi-well Luminometer (Thermo

(11) NF-κB-Driven HIV-1 Gene Expression in Human Cells Is Independent of Poly(ADP-ribose) polymerase-1 FunctionbioRxivApril 11, 2026Denisse A. Gutierrez, Manuel Llanoanalyzed for luciferase activity with a luminescence kit (Bright-Glo™ Luciferase Assay System, Promega, E2620) as described in (64). In some experiments, ATP was measured in the same samples using the CellTiter-Glo® Luminescent Cell Viability Assay

(12) Intragenic Transcription from Defective HIV Proviruses Triggers Interferon Responses in Myeloid CellsbioRxivApril 7, 2026Jonathan Kilroy, Aparna Deokar, Samantha Patalano, Juan Fuxman Bass, Manish Sagar, Suryaram Gummuluru, Andrew J. HendersonMedia/supernatant was aspirated from these plates and 30 μL of BrightGlo reagent (Promega Catalog No. E2610) were added to lyse the cells and measure luciferase activity. A standard curve was generated by adding

(13) Sialic acid cis-ligand dynamics modulate Siglec-7 and -9 function and affect Siglec-7/9 co-blockade to potentiate natural killer cell anti-tumor activityOncoimmunologyApril 2, 2026Jesper van Eck van der Sluijs, Anne H. C. Valk, Eline J. H. van Houtum, Esther D. Kers-Rebel, Maaike Looman, Willemijn Hobo, Anna E. Marneth, Harry Dolstra, Lune van den Boogaard, Christian Büll, et al.and 5% CO2, followed by luminescence measurement using Bright-Glo™ Luciferase Assay System (Promega, E2620) according to the manufacturer’s protocol on a Victor3 Multilabel Plate Reader (Perkin Elmer). Western blot

(14) TNF inhibits SARS-CoV-2 induced cell-cell fusion through activating the SDC4-RhoA signaling to promote actin bundles formationCell InsightApril 1, 2026Dong Duan, Xu Zheng, Yanqiu Zhou, Mengmeng Cui, Yunyi Li, Xiaoxian Cui, Yuying Yang, Min Chen, Huanyu Wu, Xin Chen, et al.EDTA-free protease inhibitor cocktail (Roche)). Lysates were mixed with Bright-Glo luciferase substrate (E2610, Promega), and relative luminescence units (RLU, 1-min read) were measured using a Synergy H1 plate

(15) Receptor-guided AAV Tropism Engineering via MATCHbioRxivApril 1, 2026Nolan Graham, Satheesh Kumar, Joseph Rainaldi, Seoin Yang, Andrew Portell, Billy Santoso, Prashant MaliCK04-01) before luciferase activity was assessed using the Bright-Glo™ Luciferase Assay System (Promega, E2610) according to manufacturer’s protocols. Luciferase signal was then normalized to relative cell viability. Human

(16) Timosaponin AIII enhances CAR-T cell potency and prevents relapse through impairing CAR-TregsNature CommunicationsMarch 31, 2026Mingqi Hou, Wenjun Zhang, Ziping Qi, Guiming Li, Husheng Mei, Shuang Qi, Rui Jin, Yuedong Zhao, Xiaochen Tang, Bing Xiu, et al.Cell Viability Assay) was obtained from Vazyme (DD1101-02). The Bright-Glo™ Assay Reagent was obtained from Promega (E2620). The immobilon western HRP substrate was purchased from Millipore (WBKLS0500). The LANCETM cAMP 384

(17) Targeting oncogenic NTSR1 with liensinine reprograms Gq-mediated signaling to suppress lung adenocarcinomaChin MedMarch 25, 2026Yongfu Wang, Wei Liu, Pengzhuo Tao, Changmin Liu, Yizhen Yuan, Yajing Xue, Hanting Yang, Xiongfeng Liu, Xinyi Zhou, Shilin Chen, et al.After an 18-h incubation at 37 °C, 50 μL/well of diluted Bright-Glo Luciferase Assay reagent (Promega, E2610) was added and luminescence was measured on a plate luminometer immediately. In-cell ELISA for detection

(18) Engineering NK and T cells with metabolite-sensing receptors to target solid tumorsNature ImmunologyMarch 23, 2026Young-Min Kim, Min K. Tsai, Chang Sun, Olivia Laveroni, Reece Villarin Akana, Kristen Frombach, Livnat Jerbyeach well and incubated at 37 °C with 5% CO2 for 24 h. The next day, Bright-Glo solution (Promega, E2610) was added and incubated for 5 min at room temperature. Luminescence was measured using a Tecan Infinite

(19) Engineering programmable CAR and antigen pairing via drug-gated light activationNature CommunicationsMarch 19, 2026Ziliang Huang, Praopim Limsakul, Yiqian Wu, Tianze Guo, Yuxuan Wang, Zhuohang Wu, Linshan Zhu, Molly E. Allen, Longwei Liu, Yingxiao Wangactivity of the remaining target cells after co-culture using Bright-Glo Luciferase Assay System (Promega, E2610) following the manufacturer’s instructions. PEbody PAT CAR T killing assay: 0.1 million T cells (engineered

(20) Regulation of MRGPRX2-mediated mast cell function by competence-stimulating peptide 1 and pro-adrenomedullin peptideFrontiers in ImmunologyMarch 17, 2026Aetas Amponnawarat, Sangita Sutradhar, Chalatip Chompunud Na Ayudhya, Maram Bawazir, Ronit Sagi-Eisenberg, Hydar AliCatalog #ab104139) were purchased from Abcam. Bright-Glo Luciferase was purchased from Promega (Catalog #E2620). Dyngo-4a and Cycloheximide were obtained from Cayman Chemical (Catalog #29479 and #14126). Phycoerythrin

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Bright-Glo Luciferase Assay System

Bright-Glo Luciferase Assay System from Promega

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Promega

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Bright-Glo™ Luciferase Assay From Promega

Bright-Glo™ Luciferase Assay System From Promega

Bright-Glo Luciferase Assay System from Promega

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