Bright-Glo Luciferase Assay System E2620 from Promega

Product Specs
ItemBright-Glo Luciferase Assay System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE2620
ReactivityMammalian
Detection TargetLuciferase
SpeciesMammalian
Quantity100ml
Sample TypeMammalian cells
MethodLuciferase
SensitivityUp to tenfold more light intensity than other homogeneous luciferase assay reagents results in increased sensitivity.

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Sialic acid cis-ligand dynamics modulate Siglec-7 and -9 function and affect Siglec-7/9 co-blockade to potentiate natural killer cell anti-tumor activityOncoimmunologyDecember 31, 2026Jesper van Eck van der Sluijs, Anne H. C. Valk, Eline J. H. van Houtum, Esther D. Kers-Rebel, Maaike Looman, Willemijn Hobo, Anna E. Marneth, Harry Dolstra, Lune van den Boogaard, Christian Büll, et al.and 5% CO2, followed by luminescence measurement using Bright-Glo™ Luciferase Assay System (Promega, E2620) according to the manufacturer’s protocol on a Victor3 Multilabel Plate Reader (Perkin Elmer). Western blot

(2) Direct detection and quantification of HIV particles and their envelope trimers bound by combinations of broadly neutralizing antibodiesiScienceOctober 16, 2026Souradip Dasgupta, Aswin T. Srivatsav, Sameer Kumar, George K. Lewis, Anthony L. DeVico, Krishanu Raycommercial assays,Critical commercial assays ,, Bright-Glo™ Luciferase Assay System,Promega,Catalog #E2610 NuPAGE™ 4–12% Bis-Tris Protein Gels,Thermo Fisher Scientific,Catalog # NP0322PK2 Whatman® Optitran®

(3) Novel African Rhinolophus bat ACE2 sequences reveal the determinants of Afro-Eurasian sarbecovirus entryJournal of VirologySeptember 22, 2026Yueying Zhang, Shigeru Fujita, Masahiro Kajihara, Katendi Changula, Bernard Mudenda Hang'ombe, Shusuke Kawakubo, Spyros Lytras, Jumpei Ito, Akinori Kanai, Yutaka Suzuki, et al.respectively, and infected cells were lysed with a Bright-Glo Luciferase Assay System (Promega, Cat #E2620) 2 days post-infection. The luminescent signal was measured using a GloMax Explorer Multimode Microplate

(4) Intragenic transcription from defective HIV proviruses triggers interferon responses in myeloid cellsJournal of VirologySeptember 22, 2026Jonathan M. Kilroy, Aparna A. Deokar, Samantha Patalano, Juan I. Fuxman Bass, Manish Sagar, Suryaram Gummuluru, Andrew J. Hendersonmedia/supernatant was aspirated from these plates, and 30 µL of BrightGlo reagent (Promega Catalog No. E2610) was added to lyse the cells and measure luciferase activity. A standard curve was generated by adding

(5) A NanoLuc-Based Protease Biosensor for Highly Sensitive Detection of Intracellular Protease Activity: Applications to Apoptosis and Coronavirus InfectionBio ProtocSeptember 20, 2026Masashi Arakawa, Eiji Morita(Promega, catalog number: N1120) 2. Bright-GloTM luciferase assay system (Promega, catalog number: E2620) Lysis buffer components 1. NaCl (Nacalai Tesque, catalog number: 31320-05) 2. Tris (Tris[hydroxymethyl]aminomethane)

(6) Identification of Galectin-9 (Gal-9) as a B7-H4 binding partner and characterization of their glycosylation-dependent interaction that modulates T cell signaling within a multi-ligand/receptor networkPLoS OneSeptember 18, 2026Ravear Zhiqiang Wang, Fang Yang, Jianhua Suiwas quantified by measuring luminescence using the Bright-Glo™ Luciferase Assay System Kit (Promega, E2620). MC38-mB7-H4 stable cells were established by transducing MC38 cells with lentiviruses expressing the

(7) Suidae iPSC-derived macrophages as models for investigating susceptibility and resilience to African swine fever virusStem Cell ReportsSeptember 8, 2026Tom M. Watson, Lynnette C. Goatley, Stephen Meek, Lel Eory, Sylvia Kohler, Nicholas Berkley, Sofia Sternberg, Melany Jackson, Amy Findlay, Isabella Hoskins, et al.(Promega #E2661) and transferred to white 96 well plates. An equal volume of Bright-Glo reagent (Promega, #E2610) was added and incubated for 5 min. The plates were read on a Promega GloMax Discovery microplate reader,

(8) Microfluidic-based rapid generation of chimeric antigen receptor T cells improves low-multiplicity-of-infection transduction efficiency while preserving functional activityJ Transl MedSeptember 5, 2026Ying Jiang, Guidong Zhu, Haiyan Zhang, Dongqi TangResidual viable target-cell signal was quantified using the Bright-Glo Luciferase Assay System (Promega; E2610) on a microplate luminometer (Molecular Devices, San Jose, CA). Specific lysis was calculated as 1 -

(9) Emergence of neutralizing RBD antibodies following Omicron infection with limited activity against ancestral SARS-CoV-2iScienceAugust 21, 2026Michael S. Piepenbrink, Yao Ma, Simran Panjwani, Alexandra R. Blake, Allison M. Bell, James L. Kizziah, Sara H. Mahmoud, Gregory C. Ippolito, Nathan B. Erdmann, Paul A. Goepfert, et al.(Fc) capture kit,Cytival Life Sciences,Cat#BR100839 Bright-Glo™ Luciferase Assay System,Promega,Cat#: E2620 ,, Deposited data,Deposited data,Deposited data ,, 1332D4-S(KP3.1.1) Structure,This study,PDBID

(10) SARS-CoV-2 Neutralization Breadth Is Shaped by Variant Exposure and Immune HistoryJ Med VirolAugust 13, 2026Greta Vizgirda, Alexander P. Underwood, Ulrik Fahnøe, Christina Sølund, Signe Lysemose Villadsen, Andrea Galli, Nina Weis, Jens Bukhquantified by measuring luminescence using the Bright‐Glo Luciferase Assay System (Promega; Cat. No. E2610) and the FLUOstar Omega microplate reader (BMG Labtech). Infection was considered positive when luminescence

(11) Directed evolution of a stem-helix-targeting antibody enables MERS-CoV cross-neutralization through enhanced binding affinityPLoS PathogAugust 7, 2026Panpan Zhou, Meng Yuan, Yuexiu Zhang, Oliver Limbo, Ge Song, Fangzhu Zhao, Hejun Liu, Wan-Ting He, Tazio Capozzola, Sean Callaghan, et al.well. After 48h of incubation, luciferase activity was measured by BrightGlo substrate (Promega Cat# E2620) according to the manufacturer’s instructions. Fifty percent maximal inhibitory concentrations (IC50s)

(12) Terminus-specific antibody development and bioanalytical assay validation to quantify active DR10624 in pharmacokinetic studiesFront PharmacolAugust 3, 2026Zhenxing Zhou, Kexin Cao, Gaofeng Yao, Yuanyuan Liu, Wenwen Duan, Chengcheng Yang, Shimei Sheng, Yanshan Huangincubation, the cells were lysed using the Promega Bright-Glo™ Luciferase Assay System (catalog number: E2620; Madison, WI, USA) and luciferase activity was measured using the Molecular Devices I3 microplate reader

(13) Engineering viral protease-operated nanobodies for programmable and orthogonal control of protein functionNature CommunicationsJuly 30, 2026Mingguang Cui, Xiaoxuan Liu, Tien-Hung Lan, Tianlu Wang, Tatsuki Nonomura, Brendan McKee, Rui Wang, Yun Huang, Yubin ZhouHY-106161) were purchased from MedChemExpress. For luciferase assays, Bright-Glo Luciferase Assay System (E2610) was purchased from Promega. For SEAP assay, Diethanolamine Substrate Buffer (5X) (34064) and PNPP tablets

(14) Identification of Entacapone as a Novel β-Arrestin 1 Biased Antagonist of CXCR7MoleculesJuly 26, 2026Liangrui Shi, Yan Huang, Lian Li, Huan Li, Xin Li, Zenghao Bi, Junke Liu, Sanyin Zhang, Zhaotong Cong, Bojun Wang, et al.room-temperature PBS. According to the manufacturer’s instructions, Bright-Glo™ luciferase assay reagent (Promega, E2610) was diluted with 1 × PBS, and 50 μL was added to each well. The plate was incubated in a cell culture

(15) Strain-specific epistasis shapes fitness landscapes of APOBEC3G antagonism by HIV-1 Vif proteinsSci AdvJuly 24, 2026Caroline A. Langley, Michelle Lilly, Harmit S. Malik, Michael EmermanDEAE-dextran (20 μg/ml). At 72 hours postinfection, cells were lysed in Bright-Glo reagent (Promega, #E2610), and luciferase activity was measured on a LUMIstar Omega luminometer (BMG Labtech). Each condition

(16) Prioritizing neuroactive ligands using motif-guided virtual discovery and zebrafish profilingNPJ Drug DiscovJuly 21, 2026Ari B. Ginsparg, Jaqueline A. Martinez, Ishaan Patel, Angélique Buton, Laura T. Lee, Raheel Sarwar, Rocco Moretti, Stephanie Puig, Summer B. ThymeAfter incubation for 24 h at 37 °C, wells were treated with 5 μL of BrightGlo reagent (Promega - cat# E2610) and incubated in darkness at room temperature for 15 min. A back seal sticker (Perkin Elmer) was applied

(17) Genetic diversity of pangolin coronaviruses reveals a key immuno-evasive substitution at spike residue 519Journal of VirologyJuly 21, 2026Maximilian Stanley Yo, Yu Kaku, Yusuke Kosugi, Jarel Elgin Tolentino, Daisuke Kuroda, Yunlong Cao, Kei Satopost-infection, the infected cells were lysed with a BrightGlo luciferase assay system (Promega, Cat# E2620), and the luminescent signal produced by firefly luciferase reaction was measured using a GloMax Explorer

(18) Novel 3-Amino-2-methylquinazolinone NF-κB Inhibitors: Synthesis and Potential Anti-Inflammatory FunctionInt J Mol SciJuly 20, 2026Chrysoula Mikra, Vasiliki Petriki, Evangelos Tsioupros, Eleni Kougioumtzian, Konstantinos Michail, Stella Manta, Barry J. Campbell, Konstantina C. Fylaktakidou, Stamatia Papoutsopouloung/mL) for 3 h. Medium was removed, cells were washed with PBS, and Bright-Glo lysis reagent added (E2620—Promega; Southampton, UK). After 5 min incubation at room temperature, lysates were transferred to 96-well

(19) Capsid and integrase play essential apposing roles in viral ribonucleoprotein assembly during HIV-1 core morphogenesisiScienceJuly 17, 2026Ahinsa Ranaweera, Jonathan R. Andino-Moncada, Sarah E. Dillon, Satya P. Singh, Scott M. Stagg, Alan N. Engelman, Christopher Aiken, Ashwanth C. Francisassays,Critical commercial assays,Critical commercial assays ,, Bright-Glow Luciferase Assay System,Promega,Cat#E2620 HIV1 p24 ELISA kit,Abcam,Cat#ab218268 ,, Experimental models: Cell lines,Experimental models: Cell

(20) Cysteine Redox State Governs the Condensation Pathway of Hendra Virus W Protein and Differentially Impacts Type I IFN and NF-κB SignalingAdv Sci (Weinh)July 17, 2026Frank Gondelaud, Alexandre Lalande, Giulia Pesce, Auriane Bouchet, Carole Meunier, Christophe Bignon, Denis Ptchelkine, Yu Gu, Eva Ogire, Pierre-Yves Lozach, et al.was measured after 5 h of incubation at 37°C using the Bright‐Glo Luciferase Assay System (Promega, E2620). The medium was replaced with 50 µL per well of DMEM/10% FBS, and 50 µL per well of Bright‐Glo Luciferase

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Bright-Glo Luciferase Assay System

Bright-Glo Luciferase Assay System from Promega

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Promega

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Bright-Glo™ Luciferase Assay From Promega

Bright-Glo™ Luciferase Assay System From Promega

Bright-Glo Luciferase Assay System from Promega

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