Renilla Luciferase Assay System E2820 from Promega

Product Specs
ItemRenilla Luciferase Assay System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE2820
ReactivityRenilla
Detection TargetLuciferase
SpeciesRenilla
Quantity1,000 assays
MethodLuciferase
Sample Typemammalian cells
Sensitivity10^–19 moles of Renilla luciferase detectable.

Add to Compare List

Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) FAM134B isoform 2/RETREG1-2 defines a calnexin-TOLLIP-coupled ER-phagy pathway that restricts Ebola virus glycoprotein and is antagonized by VP40 through macro-autophagybioRxivApril 2, 2026Jing Zhang, Tao Wang, Jiaxin Wen, Jing Lan, Sunan Li, Yong-Hui Zhengp1, and p2 were lysed to quantify virus production by Renilla-Glo® Luciferase Assay System (Promega, E2820). Western blotting (WB). Transfected cells were lysed in NP-40 (Beyotime, P0013F) at 4°C After centrifugation

(2) Systematic multi-reference vertebrate ACE2 sequence similarity analysis predicts species susceptibility to SARS-related sarbecovirusesScientific ReportsMarch 18, 2026John A. Frank, Eric Xu Gan, William Brenham Hooper, Isabel Madeleine Ott, Akiko Iwasakiwere lysed, and luciferase activity was measured with the Renilla Luciferase Assay System (Promega E2820) according to the manufacturer’s instructions. Luciferase activity was measured using a BioTek Cytation 5

(3) IL-7/IL-15/IL-21 cytokine-fusion scaffold generates highly functional CAR T cells enriched in long-lived T memory stem cellsSci AdvMarch 13, 2026Erin B. Cole, Sara Lamcaj, Agnes V. Sydenstricker, Adilyn G. Voss, Christopher R. Hiner, Hong B. Hur, Manoj Kandpal, Natalia Valderrama Pena, Jian Hua Zheng, Ying Xiong, et al.by measuring relative luciferase (LucR) using the Renilla Luciferase System (Promega, reference no. E2820) as previously described (70). The percentage of HIV-1 suppression was calculated as (1 – CARRLU/HIV+RLU

(4) A multi-glycoprotein synergized recombinant virus system resolved the research dilemma of Epstein-Barr virus entry and facilitated the development of entry-specific drugs or vaccinesCurrent Research in Microbial SciencesMarch 9, 2026Tianyu Lu, Shufeng Tian, Jiapeng Luo, Zhe Zhou, Yanying Liang, Wei Xu, Shenglei Yu, Lingyun Shao, Huang Huang, Lu Lu, et al.in fresh medium for another 48 h. To detect the infection, Renilla Luciferase Assay System (Promega, E2820) was used. Briefly, cells were lysed using 100 μL of 1 × Lysis Buffer per well. Then, the cell lysate

(5) HTLV ‐1 Tax Reshapes the DNA ‐Binding Pattern of Transcription Factor IRF4 and Disrupts Host Gene RegulationCancer ScienceFebruary 25, 2026Shu Tosaka, Jun Mizuike, Motomu Yano, Kaoru Uchimaru, Makoto Yamagishiseparately with the Luciferase Assay System (Promega, #E1500) and the Renilla Assay System (Promega, #E2820) according to the manufacturer's instructions. Measurements were performed using a Centro LB 960 microplate

(6) SIRT6 Regulates Protein Synthesis and Folding Through Nucleolar RemodelingAging CellFebruary 17, 2026Daniel Stein, Christian Gallrein, Miguel Portillo, Shai Kaluski‐Kopach, Alfredo Garcia‐Venzor, Yuval Lachberg, Ekaterina Eremenko, Dmitrii Smirnov, Shiran Dror, Monica Einav, et al.luminescence readings were conducted using the Promega Renilla Luciferase Assay System kit (Promega E2820), according to the manufacturer's protocol. Protein refold capacity was calculated as the division of

(7) Derepression of transposable elements in the mouse prefrontal cortex disrupts social behaviorProc Natl Acad Sci U S ADecember 23, 2025R. Kijoon Kim, Corinne Smith, Natalie L. Truby, Shelbey R. Strandberg, Jessica L. Bell, Nic Carwile, Gabriella M. Silva, Rachael L. Neve, Theingi Aung, Xiaohong Cui, et al.normalization, followed by relative luminescence unit (RLU) using Renilla luciferase assay system (Promega, #E2820). Western Blot. Western blots were performed in cultured N2a cells and in PFC tissue homogenate. Protein

(8) HSP90 buffers deleterious genetic variations in BRCA1Molecular CellDecember 4, 2025Brant Gracia, Xing-Han Zhang, Patricia Montes, Tin Chanh Pham, Min Huang, Junjie Chen, Georgios Ioannis KarrasMycoAlert® Mycoplasma Detection Kit,Lonza Bioscience,Cat. #: LT07–318 "Renilla Luciferase Assay System, 1000 Assays",Promega,Cat. #: E2820 "SuperSignal ELISA Pico Chemiluminescent Substrate, 250 mL",ThermoFisher Scientific,Cat. #: 37069 Deposited

(9) SARS-CoV-2 polyprotein expression and the induction of double-membrane vesiclesJournal of VirologyNovember 25, 2025Meng Zhao, Yajie Zhang, Yakun Liang, Fuzhi Lei, Yuying Han, Zhenghong Yuan, Zhigang Yireplicon RNA-transfected cells was mixed with an equal volume of 2× passive lysis buffer (Promega, E2820) and analyzed for luciferase activity using the Renilla luciferase substrate (Promega, E2820) according

(10) Establishment of a stable replicon cell line of Tembusu virus (TMUV) for high-throughput antiviral screeningPoultry ScienceNovember 14, 2025Jing Wei Song, Xiao Qing Fu, Heng Xing Yuan, Peng Fei Mu, Shan Zhang, Lan Hao Liu, Pin Nietriplicate. Luciferase activity assay To detect Gluc activity, a Renilla Luciferase Assay System (Promega, E2820) and a GloMax Navigator System (Promega) were used according to the manufacturer's instruction. In brief,

(11) The miR172a‐ERF416/413 module regulates soybean seed traitsJournal of Integrative Plant BiologyNovember 1, 2025Meng Jin, Jia‐Qi Han, Lu‐Yao Zhang, Zhi‐Hao Jiang, Yue Liu, Jun‐Jie Wei, Ling‐Yi Zheng, Shang‐Shang Xiong, Yang Hu, Tong Cheng, et al.using a Luciferase 1000 Assay System (E4550; Promega, WI, USA) and a Renilla Luciferase Assay System (E2820; Promega, WI, USA), respectively. The final transcriptional activity was expressed as the ratio of LUC/REN

(12) Hepatitis E Virus ORF1 Polyprotein Harbors a Pocket‐Like Cavity That Is Vital for Virus Replication and Represents a Novel Antiviral TargetAdvanced ScienceOctober 2, 2025Xiaohui Ding, Dou Zeng, Dan Liu, Yingying Bian, Bin Li, Zheng Li, Qiudi Li, Shiquan Liang, Yunlong Si, Qili Yao, et al.Activity Assay Secreted Gluc activity was determined using the Renilla Luciferase Assay System (Promega, #E2820).[ 12 ] Briefly, 20 µL of cell culture supernatant was mixed with Renilla Luciferase Assay Reagent

(13) T cell epitope mapping reveals immunodominance of evolutionarily conserved regions within SARS-CoV-2 proteomeiScienceAugust 15, 2025Cansu Cimen Bozkus, Matthew Brown, Leandra Velazquez, Marcus Thomas, Eric A. Wilson, Timothy O'Donnell, Anna Kaminska, Denis Ruchnewitz, Douglas Geertz, Yonina Bykov, et al.MC10054-01; MC10038-01; MC10026-01; MC10022-01; MC10012-01 Renilla Luciferase Assay System,Promega,E2820 ,, Experimental models: Cell lines,Experimental models: Cell lines,Experimental models: Cell lines ,, ACE2+

(14) Cryo-EM structures of Nipah virus polymerase complex reveal highly varied interactions between L and P proteins among paramyxovirusesProtein & cellAugust 7, 2025Xue L, Chang T, Gui J, Li Z, Zhao H, Zou B, Lu J, Li M, Wen X, Gao S, et al.cell culture supernatant was harvested. After preloading 100 μL of Renilla Luciferase Assay Reagent (Promega, E2820) into a 96-well opaque, white plate, 20 μL of cell culture supernatant was carefully added and mixed

(15) MFSD6 is an entry receptor for enterovirus D68NatureMay 1, 2025Varanese L, Xu L, Peters CE, Pintilie G, Roberts DS, Raj S, Liu M, Ooi YS, Diep J, Qiao W, et al.the media was removed, and 60 μL of lysis solution from the Renilla Luciferase Assay kit (Promega, E2820) was added. Lysates were stored at −20°C. Once all cells were harvested, lysates were thawed and 20

(16) Distinct pathogenic mutations in ARF1 allow dissection of its dual role in cGAS-STING signallingEMBO ReportsMarch 24, 2025Johannes Lang, Tim Bergner, Julia Zinngrebe, Alice Lepelley, Katharina Vill, Steffen Leiz, Meinhard Wlaschek, Matias Wagner, Karin Scharffetter-Kochanek, Pamela Fischer-Posovszky, et al.and GAPDH-Rluc activity was measured utilizing the Promega Renilla luciferase assay system (Promega, E2820), following the manufacturer’s instructions. Measurements were performed with the Orion II microplate

(17) Druggable genome screens identify SPP as an antiviral host target for multiple flavivirusesProceedings of the National Academy of SciencesFebruary 25, 2025Wenjie Qiao, Xuping Xie, Pei-Yong Shi, Yaw Shin Ooi, Jan E. Caretteactivity was measured at the indicated time points using the Renilla Luciferase Assay System (Promega, E2820) or Nano-Glo Luciferase Assay System (Promega, N1120) with a GloMax luminometer. Effective concentrations

(18) miR-193b-3p suppresses lung cancer cell migration and invasion through PRNP targetingJ Biomed SciFebruary 20, 2025Hsiang-Ling Ho, Shin-Chih Lin, Chao-Wei Chiang, Ching Lin, Che-Wei Liu, Yi-Chen Yeh, Mei-Yu Chen, Teh-Ying Chouusing the Luciferase Reporter System (Promega, Cat. #E1501), with Renilla luciferase (Promega, Cat. #E2820) as a control. Firefly luciferase activity was normalized to Renilla luciferase activity. Primary and

(19) TAZ (WWTR1) Interactome Analysis using nanotrap based affinity purification-mass spectrometryJournal of Cell ScienceFebruary 15, 2025Jonathan Kelebeev, Anastasia MacKeracher, Tetsuaki Miyake, John C. McDermottTechnologies) using luciferase assay substrate (Promega, #E1501) or Renilla assay substrate (Promega, #E2820). Luciferase activity values were normalized to Renilla activity values in the same cell extracts and

(20) Replication-incompetent VSV-based vaccine elicits protective responses against SARS-CoV-2 and influenza virusScience advancesJanuary 31, 2025Adeleke RA, Sahler J, Choi A, Roth K, Upadhye V, Ezzatpour S, Imbiakha B, Khomandiak S, Diaz A, Whittaker GR, et al.luminescence produced by the virus-encoded Renilla luciferase using the Renilla luciferase assay kit (Promega, E2820). Plaque reduction neutralization test Half-log dilutions of serum samples (60 μl per well) from immunized

Write a Review

(1)

3/26/2018Renilla Luciferase Assay System Alex RayOur lab utilizes this system to detect HIV infection levels using virus that expresses luciferase. The assay system is very sensitive and easy to get up.
(1)

3/1/2017Renilla Luciferase Assay System Review Lana JagannathanOur lab uses HIV molecular clones that express Renilla luciferase reporter. We infect cells in vitro, or perform in vivo experiments where we infect our mice with these viruses. As a read out, we test... Read Review
(1)

2/27/2017Renilla Luciferase Assay Kit Nina FlerinOur laboratory investigates different ways to improve immune responses against HIV, including enhancement of the capacity of effector cells, such as NK cells, to kill HIV-infected cell. In our ... Read Review

Renilla Luciferase Assay System

Renilla Luciferase Assay System from Promega

Description

Promega

Citations (124)

Reviews

Renilla Luciferase Assay System from Promega

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map