Promega
Dual-Luciferase Reporter Assay System
E1910
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This reporter system was used in conjunction with an SRF-RE/firefly luciferase reporter construct to monitor the ability of a pharmaceutical compound to stimulate G12/G13 signalling in the AT1 receptor using A7r5 cells. The kit is easy to use and accurate.
G12/13 signalling measured by luciferase luminescence.
AT1 receptor-transfected A7r5 cells
AT1 receptor-transfected A7r5 cells were additionally transfected with an SRF-re/firefly luciferase constructs and a renilla luciferase construct. After incubation and stimulation with a compound to induce G12/13 signaling, the Dual-Luciferase Reporter Assay System was used according to manufacturer specifications and read on a Mithras LB 940 reader.
All reagents were prepared fresh
A reproducible dose dependent curve for G12/13 signaling was created using this system.
N/A
Easy to use with accurate results due to renilla control.
No downsides.
Would highly recommend. Very easy to use and highly reproducible results.