Promega
Dual-Luciferase® Reporter Assay System
E1960
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The Dual-Luciferase Reporter (Promega) Assay System provides an efficient means of performing two reporter assays. In the assay, activities of firefly and Reilla luciferases are measured sequentially from a single sample.
Luciferase Reporter Assay
HEK293 Whole Cell Lysate
Cells were first transfected with plasmid (Luciferase Reporter Vector ) of interest along with Renilla luciferase vector. After 36 hours of transfection, cells lysed with 50 microlitre (per well/24 well plate) of 1 X Passive Llsis buffer. 10 microlitre of each sample has taken in 1.5 ml centrifuge tube and 50 microlitre of Luciferase Assay Reagent II added to generate luminescent signal, which was measured by luminometry. Immediately, 50 microlitre of Stop & Glo reagent was added to the same tube to quench the reaction and initiate the Renilla luciferase reaction, which was also measured by Luminometry. The relative luciferase activity was measured by normalizing with Renilla luciferase activity.
50 microlitre of Luciferase Assay Reagent and 50 microlitre of STOP & Glo is enough for measurement
The kit produces high counts of luminometry measurements of both firefly luciferase and renila lunicferase activity.
N/A
Measurement reading is high when compare to using other kits.
Nothing
Good kit for Dual Luciferase Assay
Product Name: Dual-Luciferase Reporter Assay System 10-PackSupplier Page