Anti-Vimentin antibodies are used for the immunodetection of the protein encoded by the VIM gene. In humans, the canonical protein has a reported length of 466 amino acid residues and a mass of 53.7 kDa. Its subcellular localization is in the cell membrane, nucleus, and cytoplasm. It is reported to be highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. A member of the Intermediate filament protein family, it is known to be involved with Bergmann glial cell differentiation and the cellular response to lipopolysaccharides. Post-translational modifications have been described, including O-glycosylation and phosphorylation.
The vimentin marker can be used to characterize Interstitial Like Cell, Fibroblasts, and Type 2 Medullary Thymic Epithelial Cells.* Other names for this target antigen include epididymis secretory sperm binding protein. Gene orthologs have been identified in the mouse, rat, bovine, frog, zebrafish, chimpanzee and chicken species.
*HuBMAP Human Reference Atlas v1.4