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Biocompare
Next Generation Screening and Functional Validation of Antigen-Specific TCRs
Next Generation Screening and Functional Validation of Antigen-Specific TCRs
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Alex Chenchik, Ph.D.
President and CSO, Cellecta Inc.
April 20, 2026
Chapters
Introduction
TCR and BCR Immune Receptors: Universal Diagnostic System
Technological Platform to Study Immune Receptors
Adaptive Immune Receptor (AIR) Profiling Assays
AIR-RNA Assay Outline
Single-Cell AIR TCR/BCR Phenotyping Assay in Sorted T/B cells
Bulk AIR Repertoire Profiling. Quantification of AIR profiling Data.
Comprehensive AIR-RNA Repertoire Analysis in Whole Blood and PBMC
TRB Clonotype Repertoire Analysis in Blood Samples
How to Identify Activated Immune Receptor Clonotypes?
How to Identify Activated Immune Receptor Clonotypes?
Characterization of Immune Responses after Vaccination
DriverMap Genome-wide 19K Expression Profiling
AIR TCR/BCR Repertoire Analysis in Blood RNA Samples
Prediction of Vaccine Epitopes with ImmuneWatch DETECT Algorithm
Screening of Functional TCR-Peptide Interactions
Screening of MART-1 Peptide-Activated TCRs in Donor PBMC
Selection of MART-1 Activated TCR Clonotype Candidates
Screen for Low-frequency TCRs with NY-ESO-1 Stimulation
Detection of Functional TCR-Epitope Interactions with Jurkat-NFAT-GFP and K562-HLA cells
Functional validation with Jurkat-K562 Interaction Assays
Discovery of Functional TCR-Epitope Interactions Stimulation of PBMCs with Peptide(s) in vitro
High-throughput Screening of Functional TCR-Epitope Interactions with FluoroSPOT Microwell Array