Great Vimentin Antibody for Western Blot

July 09, 2018

Central Laboratory
Shin-Kong Wu Ho-Su Memorial Hospital
Principal Investigator

Overall

Quality of Results

Ease-of-Optimization

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Company:

GENETEX

Product Name:

Vimentin antibody

Catalog Number:

GTX100619

This antibody was used to detect Vimentin expression in MG63 cell line by western blot analysis. Great antibody, and worth the cost.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human osteosarcoma cell line whole cell lysate

Primary Incubation

1:100 dilution overnight at 4 degree in TBST buffer

Blocking Agent

WB:5% non-fat dry milk in TBST

Secondary Incubation

1:100 dilution overnight at 4 degree in TBST buffer

Tertiary Incubation

N/A

Detection

HRP substrate

Results Summary

This antibody perfectly detected Vimentin expression by western blot analysis in human osteosarcoma cell line (MG63).

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

High sensitivity

The Bad

Minor background signals

The Bottom Line

We will continue to use this antibody

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