Santa Cruz Biotechnology
Vimentin Antibody (V9)
sc-6260
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The purity of the stromal preparations was confirmed by positive cellular staining for vimentin in human primary endometrial cells. Therefore, Vimentin Antibody (V9) was used to check the purity of stromal cells.
Immunofluorescence
Primary human endometrial cells
1:250
5% BSA
FITC labelled antibody 1:500
NA
Florescent microscope
By using Vimentin Antibody (V9), it was found that most of the cells are vimentin positive and this result indicates that these cells are pure stromal cells. was used to check the purity of stromal cells.
No need of optimization
Overall this antibody stains all the vimentin-positive stromal cells present in the cell culture.