Abcam
Anti-Vimentin antibody
ab8978
N/A
Info:Supplier Page View Product Specs
Browse Similar Products: Vimentin Antibodies
Epicardial cells were differentiated from hESC cells and grown in 2D monolayers. Cells were treated with TGF-beta to induce epithelial to mesenchymal transition and then fixed and stained for vimentin. Vimentin staining was strongly positive in cell cytoplasm.
Immunofluorescence
Human embryonic stem cell derived epicardial cells
5ug/ml in 10% normal goat serum, overnight at 4 degrees
10% normal goat serum, 1 hour, room temperature
Goat Anti-mouse AF488 IgG H&L (ab150113) (1:500)
Olympus Fluorescent microscope, FITC channel
TGF-beta treated epicardial cells stained positive for vimentin in the cytoplasm. Staining was easy to optimize with little effort and showed strong signal relative to background.
Fixed cells were permeabilized with 0.25% Triton-X, 15 min, room temperature.
Easy to optimize, strong signal relative to background.
Expensive for size of product.
Good choice for vimentin staining, will be using this product again.