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Biocompare
High content live cell assays for immunology
High content live cell assays for immunology
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Guido Stadler, Ph.D.
Director of Live Cell Biology,
Celldom
April 20, 2026
Chapters
Introduction
Overview
Next-Generation Live Cell Assay Platform
Anatomy of a cloneXpress cell culture plate
Priming submerges the microwell array in culture medium
Simple cell loading
cloneXplorer: live cell imager with clone picker
Timelapse Image Analysis
Recovery of cells from microwells based on specified characteristics
Image Documentation at every step
T cell activation measured by IFN-γ secretion
No wash kinetic Fluorospot assay
TCR Screening of Antigen Libraries
Discover and validate TCRs in as little as 1 month
Isolation of antigen-specific T cell lines
TCR-seq and validation of isolated T cell lines
CAR-T potency assay
Killing analysis of single T cells
Key features of Celldom live cell assays
Validated immunology applications
Acknowledgments