Abcam
Recombinant Anti-Vimentin antibody [EPR3776]
ab92547
N/A
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Currently working with cell culture and doing immunocytochemistry for specific cell markers, such as vimentin, to confirm the cell type after different treatments.
Immunofluorescence
Amnion epithelial and mesenchymal cells, chorion trophoblast cell
1:300, overnight incubation at 4 degrees, rocking
3% BSA-PBS
1:1000, 1 hour, Rabbit Alexa Fluor 488
DAPI in PBS, 5 min
Keyence microscope
Dilution and incubation time works fine. Expected too see "filaments like" in green. Looks good in the cell mentioned above even after different types of treatments.
Easy to use; good quality.
None
Good quality antibody; signal is good even with 1:300 dilution. A good market for these types of cells.