Time Course Effect of Vimentin by Chemoattractant

Department of Pharmacology and Toxicology
Augusta University
Postdoctoral Researcher

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Company:

Santa Cruz Biotechnology

Product Name:

Vimentin

Catalog Number:

SC-6260

Clone Number:

V9

Dichloroacetate inhibition of PDK frees up the mitochondrial gatekeeping enzyme PDH, which is then able to convert pyruvate to acetyl-CoA and initiate normal oxidative phosphorylation via the TCA cycle. Here we checked the time course effect of Vimentin by chemoattractant.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Rat tissue lysate

Primary Incubation

1:1000 dilution in 5% skim milk prepared in 1X TBST

Blocking Agent

5% skimmed milk

Secondary Incubation

1:4000 5% skim milk prepared in 1X TBST

Tertiary Incubation

None

Detection

ECL

Results Summary

We found time dependent increase of vimentin by chemoattractant.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

No background and very strong signal

The Bad

None

The Bottom Line

This antibody signal is strong. Highly recommended to others.

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