Great Vimentin Antibody for Western Blot

May 09, 2022

Nanomedicine
Lazar Research Laboratories
Clinical Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Recombinant Anti-Vimentin antibody

Catalog Number:

ab92547

Clone Number:

N/A

I am very satisfied with this antibody. I tested it on endothelial cells (A549) stimulated with TGF-b for 24 hours in starvation medium (F-12k). The bands have been very well detected.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Epithelial cells

Primary Incubation

1:1000, 4 °C, overnight

Blocking Agent

BSA 5% TBST

Secondary Incubation

1:10000

Tertiary Incubation

N/A

Detection

Chemiluminescence

Results Summary

I have never had a problem with this antibody. The bands are very strong, I recommend diluting the antibody even 1: 10000.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Clear band

The Bad

N/A

The Bottom Line

I recommend to buy/use it and to dilute up to 1:10000.

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