Santa Cruz Biotechnology, Inc.
Vimentin
sc-6260
V9
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Vimentin phosphorylation and assembly are regulated by the small GTPase Rab7a. This antibody is highly specific for Vimentin.
Western Blot
Cell lysate
1:1000 dilution in 5% skim milk prepared in 1x TBST
5% skim milk
1:4000 5% milk
None
ECL
Rab7a silencing causes an increase of vimentin present in the insoluble fraction (assembled). Also, expression of Charcot–Marie–Tooth 2B-causing Rab7a mutant proteins induces vimentin phosphorylation and increases the amount of vimentin in the soluble fraction. Thus, modulation of expression levels of Rab7a wt or expression of Rab7a mutant proteins changes the assembly of vimentin and its phosphorylation state indicating that Rab7a is important for the regulation of vimentin function.
N/A
No background and strong signal
This antibody is highly specific and works great for western blot. Highly recommended.