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Date Published
Anti-CD66b BV421 Works Great
(1)
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April 14, 2021
This antibody was used to stain for CD66b expression in human peripheral blood. It was chosen because it is a commonly used clone for CD66b,...
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Great Alexa Fluor 546 Goat Anti-Rabbit
(1)
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Thermo Fisher Scientific
April 14, 2021
We used this antibody as secondary antibody for IF in cell lines and mice tissue sections.
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CD45.2 Antibody Gives Good Results
(1)
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April 14, 2021
CD45.2 is routinely used a congenic marker in mouse immunology as it is found all on all hematopoietic cells. We use this marker to identify...
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Great Gzmb+ Staining
(1)
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Thermo Fisher Scientific
April 14, 2021
We wanted to examine cytotoxic T cells in the healthy vs diseased state, so we used this antibody to identify Gzmb+ T cells in the spleen.
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Robust and Easy to Use Kit
(1)
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RayBiotech
April 13, 2021
Our lab used these kits on over 70 samples of SARS-CoV-2 convalescent plasma and they worked great. Little optimization was required outside...
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Consistent and Easy to Use
(1)
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Mabtech AB
April 12, 2021
These kits were used with SARS-CoV-2 convalescent sera samples as well as a negative control sera pool and a positive control sera pool. Our...
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Great Antibody to Analyze CD69 Expression
(1)
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April 12, 2021
Acute T cell activation induces strong expression of CD69 as a marker of early activation. We use this antibody in our flow cytometry ...
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Hoechst 33258 Solution 1 mg/mL in H2O
(1)
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Thermo Fisher Scientific
April 12, 2021
Cells were extensively washed with PBS1x before adding Hoechst 33258. A dilution of 1 uL/ml is working. If adding more, it is better to ...
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Works Well to Detect CTLA-4 Expression
(1)
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Thermo Fisher Scientific
April 12, 2021
As a cancer immunology lab, we study exhaustion in T cells within the tumor microenvironment. CTLA4 is a classic marker of exhaustion as it ...
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CoV2 NC ELISA Kit Review
(1)
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RayBiotech
April 12, 2021
We are developing a COVID 19 N Ag rapid test. This kit provides a limit of detection comparator.
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APC Anti-mouse Works Great
(1)
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BioLegend
April 12, 2021
To titrate the antibody, 1 x 10^6 splenocytes from C57BL/6 mouse infected with LCMV-C13 (day 8) were stained with: AF700 anti-CD8a Ab in the...
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Works Great for Measuring S6 Ribosomal Protein Expression
(1)
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Cell Signaling Technology
April 12, 2021
Aim of the study was to test the time course effect of a specific growth factor on S6 Ribosomal protein levels in mouse primary cells. The ...
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MIG ELISA for Cell Culture Supernatants and Serum
(1)
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RayBiotech
April 11, 2021
This kit was ordered for use in detecting Mouse MIG from cell culture supernatants and mouse serum.
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DAPI Solution Works Great
(1)
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Thermo Fisher Scientific
April 11, 2021
Cells were washed with PBS1x before the addition of DAPI (1uL/1ml) in 1xPBS. 30 minutes of incubation was followed by wash with PBS1x and ...
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Good Quality Xanthine Oxidase Antibody
(1)
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Abcam
April 10, 2021
Human cells were growth-arrested, treated with a specific growth factor; protein extracts were made and resolved in a 10% SDS PAGE. Resolved...
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Antibody Stains HLA-ABC Very Well
(1)
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Novus Biologicals, a Bio-Techne Brand
April 08, 2021
Our group used this antibody to detect the HLA-ABC expression in frozen tissue sections (ulcer) by immunfluorescence.
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Great FITC Anti-Human CD16 Antibody
(1)
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Bioss Inc.
April 08, 2021
I used this antibody to detect the CD16 positive cells in frozen tissue sections (ulcer) using IF.
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Works Well for Immunodetection of CD36 Protein
(1)
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MyBioSource.com
April 08, 2021
To measure Caspase1 levels in a mouse cell line treated with a growth factor, mouse cells at log phase were treated with the growth and ...
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Great Non-Ionic Surfactant and Emulsifier
(1)
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MilliporeSigma
April 08, 2021
Worked great with buffer for cell lysis in immunoprecipitation. Include in your wash buffer as well to get rid of access antibodies.
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Great Permeabilizing Agent for Immunofluorescence
(1)
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MilliporeSigma
April 08, 2021
Really good permeabilizing agent. Works with antibodies that do not seem to work with Triton X. Also gentle with cellular structures and ...
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Good Monoclonal anti-IgA
(1)
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Thermo Fisher Scientific
April 08, 2021
Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against ...
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Works Well for Staining of Human Keratinocytes
(1)
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Miltenyi Biotec
April 07, 2021
For cyropreserved skin stainings our group always use this antibody to vizualize the epidermis. This antibody works very well without ...
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Anti-CD29 Antibody Works Great
(1)
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Novus Biologicals, a Bio-Techne Brand
April 07, 2021
We wanted to look at expression levels of different integrins in venous leg ulcer, so we used this antibody to stain for CD29.
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Vimentin Antibody Works Well
(1)
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Novus Biologicals, a Bio-Techne Brand
April 07, 2021
In the present study, Vimentin expression was detected in venous ulcer. Selection of antibody was based on previous experience.
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Miltenyi Antibody Stains CD39 Well
(1)
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Miltenyi Biotec
April 07, 2021
We used this CD39 antibody to detect certain immune subsets in venous ulcer using immunofluorescence.
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Detection of CD63 Positive Cells in Ulcus
(1)
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Miltenyi Biotec
April 07, 2021
The aim of my research is to study the compositon of immune cells and different proteins in venous ulcus. In my experiment we used a novel ...
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Great Quality D + Glucose
(1)
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MilliporeSigma
April 07, 2021
Worked great with cell culture media formulations. Also used in making buffer for live cell imaging of human primary and mouse primary cells...
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Good Specificity for Immunodetection of FLT4
(1)
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Thermo Fisher Scientific
April 06, 2021
SDS-PAGE resolved human protein extracts from cell line exposed to different time periods of a growth factor were immunoblotted and probed ...
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Immuno-Detection of CPT1B Works Well
(1)
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Novus Biologicals, a Bio-Techne Brand
April 06, 2021
On-target siRNA transfected human cells treated with a growth factor for a specific time point were subject to screen the effect of siRNA on...
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Quality Imidazole for Buffers
(1)
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MilliporeSigma
April 06, 2021
Use it in buffers to elute His tag proteins from nickel column. The reagent is very pure and gives optimal elution when used in right ...
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Great Antifade for Super-Resolution Microscopy
(1)
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Thermo Fisher Scientific
April 06, 2021
ProLong Diamond antifade is great in preserving fluorescence across whole visible and IR wavelength. Glycerol based liquid mount can be ...
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The Antibody Stains CD19+ Cells Well
(1)
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BioLegend
April 01, 2021
We wanted to examine B cells in the healthy vs diseased state, so we used this antibody to identify B cells in the blood.
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Anti-Human CD14-FITC Monoclonal Antibody for IF
(1)
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Miltenyi Biotec
April 01, 2021
In this project we are studying the immune infiltration in venous ulcer . In the experiment we used a staining technique where you can stain...
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Very Good CD107a Antibody for Immunofluorescence
(1)
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Novus Biologicals, a Bio-Techne Brand
April 01, 2021
We are seeking to understand the pathogenesis of venous ulcer. Here, we analyzed the presence of CD107a in a frozen tissue section.
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MACSQuant® Tyto® Cell Sorter Works Well
(1)
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Miltenyi Biotec
March 30, 2021
We had great success using the Tyto to sort for Nuclei from multiple tissue types (both human and mouse). The Tyto is extremely user ...
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Excellent Anti-CD1a Antibody
(1)
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Novus Biologicals, a Bio-Techne Brand
March 30, 2021
The aim of my research is to study the pathogenesis of venous ulcer. Here, we determined the presence of CD1a cells in a frozen tissue ...
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Best Antibody for Immunodetection of ACC in Humans
(1)
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Cell Signaling Technology
March 30, 2021
Mammalian smooth cells in the log phase of growth were synchronized, and for a different period, these cells were treated with a specific ...
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Good Antibody for Immunodetection of p53
(1)
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Cell Signaling Technology
March 30, 2021
Cells from human cell line was subjected to specific growth factor treatment, and cell extracts were prepared. An equal amount of proteins ...
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Anti-CD66b FITC Works Great
(1)
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BioLegend
March 28, 2021
This antibody was used to stain for CD66b expression in human peripheral blood. It was chosen because it is a commonly used clone for CD66b,...
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Best Immunodetection of Phospho-CREB Protein Levels
(1)
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R&D Systems, a Bio-Techne Brand
March 28, 2021
Aim of the experiment was to measure the study state levels of Phospho-CREB in control and growth factor treated human cell line. The ...
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Not the Best p53 Antibody
(1)
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MyBioSource.com
March 28, 2021
To test the time course effect of particular growth factor on p53 expression in mouse, the primary cell lines were growth-arrested for 12 ...
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The AKTA Pure Enables Automated Protein Purification
(1)
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March 28, 2021
The AKTA pure allows for automated purification when an appropriate method is configured. It can load and elute up to 7 samples in one run.
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Biorad Prestained Protein Standard is a convenient for SDS-PAGE standard
(1)
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Bio-Rad
March 28, 2021
When loading about 10ul of ladder onto an SDS-PAGE gel you get nice clean bands with clear resolution. Being prestained saves time and ...
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Works Great to Fix and Permeabilize Cells
(1)
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Thermo Fisher Scientific
March 26, 2021
The goal was to fix and permeabilize cells for staining intracellular protein for flow cytometry analyses.
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CCL2 Expressed by Fibroblasts
(1)
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March 26, 2021
The goal was to detect the expression of CCL2 in skin fibroblasts. This antibody worked well.
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Great Alexa Fluor 488 Goat Anti-Mouse antibody
(1)
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Thermo Fisher Scientific
March 26, 2021
We have been using this Alexa Fluor 488 Goat Anti-Mouse as secondary antibody for IF or ICC.
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Excellent Anti- Phospho-Cofilin (Ser3) antibody for Immunodetection in human cell
(1)
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Thermo Fisher Scientific
March 26, 2021
Aim of the study was to test the effect of treatment of a growth factor to cells on phospho-Cofilin levels. The cells were treated with the ...
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Excellent Anti- CUL1 Antibody
(1)
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Cell Signaling Technology
March 26, 2021
Aim of the study was to test the effect of an on-target siRNA on CUL1 protein levels in human cells. The transfected cells were transfected ...
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Mouse anti-Human Kappa Light Chain Secondary Antibody - APC - FACS stain
(1)
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Thermo Fisher Scientific
March 25, 2021
Two cell lines were stained in 3 different dilutions to establish working conditions.
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Thy1.2+ Cells Works Well
(1)
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BioLegend
March 25, 2021
We wanted to examine T cells in the healthy vs diseased state, so we used this antibody to identify T cells in the blood.
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Injector
Kits
Labels / Dyes / Stains
Ladders and Markers
Light Sources
Liquid Handling Equipment
Luminometer
Magnetic Beads
Magnets
Mass Spectrometer
Master Mixes
Media
Meter
Microplate Washer
Microscopes
Microtome
Mixer
PCR Equipment
Pipettor
Plate Reader
Precast Gels
Primary Cells
Proteins
Purification Equipment
Refrigerator / Freezer
Rocker
Sample Prep Equipment
Scanners
Services
Shaker
Slides
Software
Sonicator
Spectrometer
Sterilizer
Stirrer
Substrates
Transfection Equipment
Vacuum Manifolds
Vortexer
Water Bath
Applications
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Western Blot
Flow Cytometry
ELISA
Immunofluorescence
Immunoprecipitation
Immunocytochemistry
Immunohistochemistry
Block/Neutralize/Inhibit
Cell Isolation
Dot Blot
ChIP
Purification
Staining
Cell Culture
DNA Purification
PCR
RNA Analysis
RNA Purification
qPCR
Clean-up
Microscopy
Cloning
RT-PCR
cDNA Synthesis
Transfection
Electrophoresis
RT-qPCR
Cell Counting
Protein Purification
Next Gen Sequencing (NGS)
Library Construction
Genotyping
Gel Imaging
Protein Assay
Reporter Assay
Cell Lysis
Multiplex
Gel Staining
RNA-Seq
Live Cell Imaging
Cell Differentiation
Ligation
Concentration
HPLC
Sequencing
Chromatography
Mass Spectrometry
Labeling
Microarray
Mutagenesis
FISH
CRISPR
TUNEL Assay
In Vivo Imaging
EMSA
Cell Fractionation
Immunoassay
In Vitro Transcription
ChIP-Seq
Northern Blot
3D Imaging
RNAi
ELISPOT
PCR Cloning
Particle Analysis
Southern Blot
Protein Modification
DNA Analysis
Expression
Cell-Based Assay
Gene Editing
In Vivo Testing
Protein Labeling
Other
Species
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Human
Mouse
Rat
Non-Human Primate
Rabbit
Sheep
Hamster
Chicken
Bovine
Canine
Porcine
Horse
Monkey
C. elegans/Worm
Insect
Amoeba/Protozoa
Bacteria/Archaea
Arabidopsis
Yeast/Fungi
Squirrel
Fish
Virus
Other Invertebrate
Xenopus/Amphibian
Drosophila
Goat
Rhesus
Tissue
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Adipose
Aorta
Artery
Bladder
Blood
Blood Vessel
Bone
Bone Marrow
Brain
Breast
Bronchus
Cerebral Cortex
Colon
Cornea
Coronary Artery
Dermis
Duodenum
Ear
Embryo
Endometrium
Epidermis
Eye
Foreskin
Gingiva
Hair
Heart
Hippocampus
Hypothalamus
In Vivo
Intestine
Kidney
Large Intestine
Liver
Lung
Lymph Node
Mammary Gland
Mouth
Muscle
Ovary
Pancreas
Peripheral Blood
Peritoneum
Placenta
Prostate
Rectum
Retina
Skeletal Muscle
Skin
Small Intestine
Spinal Cord
Spleen
Stomach
Striatum
Synovium
Tail
Teeth
Testis
Thymus
Thyroid Gland
Tonsil
Trachea
Umbilical Cord
Umbilical Vein
Uterus
Vein
Cell Type
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Adipocyte
Astrocyte
B Cell
CD8+ T Cell
Competent Cells
Dendritic Cell
Embryonic Stem Cell
Endothelial Cell
Eosinophil
Epithelial Cell
Fibroblast
Glial Cell
Granulocyte
Helper T Cell
Hematopoietic Stem Cell
Hepatocyte
HUVEC
Induced Pluripotent Stem Cell
Keratinocyte
Leukocytes
Lymphocyte
Macrophage
Mesenchymal Stem Cell
Microglia
Microvascular Endothelial Cell
Monocyte
Myoblasts
Myocyte
Neuron
Neutrophil
NK Cell
NKT Cell
Oligodendrocytes
Osteoclast
PBMC
Platelets
Progenitor Cell
Red Blood Cell
Regulatory T Cells
Skeletal Muscle Cell
Smooth Muscle Cell
Splenocyte
Stem Cell
Stromal Cell
Synoviocyte
T Cell
Thymocytes
Tumor Cells
Cell Line
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HeLa
PC3 (PC-3)
HEK293
HEK293T (HEK-293T)
CHO
Jurkat
3T3
Caco-2
HepG2
THP-1
U2OS
PC12 (PC-12)
HL60 (HL-60)
A375 (A-375)
MOLT4 (MOLT-4)
MCF-7
A431
MDA-MB-231
A549
N2a
Daudi
HCT-116
Raji
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