Santa Cruz Biotechnology
CUL-2 Antibody (C-4)
sc-166506
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Human cells were treated with growth factor for a different period's cells were lysed in RIPA buffer, and proteins were extracted. The extracted proteins were resolved in 10% denaturing gel and western transferred in the nitrocellulose membrane. Further, the membrane was probed with culin2 antibody to test the effect of growth factor treatment on culin2 expression levels. The Santa Cruz Biotechnology culin2 antibody selection was based on our previous experience.
Western Blot
Human whole-cell protein lysate
Dilution (1:500) in 5% skimmed milk prepared in 1x TBST, overnight incubation at 4 degree Celsius
5% skimmed milk made in 1x TBST
Goat anti-mouse IgG HRP, Dilution (1:4000) in 5% skimmed milk made in TBST, 1hr at RT
None
Enhanced chemiluminescent detection in X-Ray film
A very faint band of CSN3 protein was detected by this antibody. Non-specificity of this antibody is extreme and bright signals of other epitopes were also detected. However, there was no time-dependent effect of expression of culin2 protein was observed.
Although culin2 band was detected antibody also detect non-specific bands.
Poor antibody for Immunoblotting culin2 in human cells