Reliable Anti-mouse CD8a-PerCP/Cy 5.5 Antibody

Vaccine Immunology Laboratory
National Institute of Immunology, India
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

PerCP/Cyanine5.5 anti-mouse CD8a Antibody

Catalog Number:

100733

Clone Number:

53-6.7

We used this product to study immune cells in control and treated mouse splenocytes single-cell suspension. Specifically, Cytotoxic T cells (CD8+) were distinguished using this antibody It fits well in our antibody cocktail for immuno-phenotyping. The antibody is specific and works well.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

mouse splenocytes

Primary Incubation

1:200 at 4 degree C for 20 minutes

Blocking Agent

Fc-block

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

Distinct CD8 positive and negative cloud was observed,

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Reliable and good signal

The Bad

None

The Bottom Line

The good antibody and also recommended for multi-color immunophenotyping

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