Excellent Anti-mouse/Human B220-AF700 Antibody

May 10, 2021

Vaccine Immunology Laboratory
National Institute of Immunology, India
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Biolegend

Product Name:

Alexa Fluor® 700 anti-mouse/human CD45R/B220 Antibody

Catalog Number:

103231

Clone Number:

RA3-6B2

To distinguish T and B cells in mouse splenocytes, we have used this antibody.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

mouse splenocytes

Primary Incubation

1:400 at 4 degree C for 20 minutes

Blocking Agent

Fc-block

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

B cells are detected as distinct cloud positive for AF700 staining in red laser, confirming B cells' presence in mouse splenocytes single cells suspension.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Good signal and can be used at lower dilutions

The Bad

None

The Bottom Line

Highly recommended

Share your experience with other scientists. Write a Review! »

Join the discussion