Insulin B Antibody for Immunocytochemistry

May 10, 2021

Natural Sciences
Sungkyunkwan University
Graduate Student

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Company:

Santa Cruz

Product Name:

Insulin B antibody (C-12)

Catalog Number:

sc-377071

Clone Number:

N/A

I used this antibody to check for Insulin B expression in miRNA transfected Capan-1 cells via Immunocytochemisty. Fixation: 4% PFA - 10 minBlocking: 2% BSA - overnightPrimary antibody dilution: 1:100Secondary antibody dilution: 1:300 (Goat anti-Mouse IgG (H+L) Cross-Adsorbed ReadyProbes Secondary Antibody, Alexa Fluor 594 - Invitrogen)

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Capan-1

Primary Incubation

1:100 (Anti-Insulin B- Santa Cruz, Anti- Neurogenin 3 - Novus)- overnight

Blocking Agent

Bovine Serum Albumin (2%)

Secondary Incubation

1: 300 (Goat anti-Mouse IgG (H+L) , Alexa Fluor 594) and (Goat anti-Rabbit IgG (H+L) , Alexa Fluor 488)

Tertiary Incubation

DAPI

Detection

Fluorescence microscope

Results Summary

When compared to the cells without transfection, I could see Insulin B expression in miRNA transfected cells.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Reliable and good signal

The Bad

A bit expensive

The Bottom Line

Anyone looking for Insulin B expression

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