Really Good ATG5 Antibody

May 11, 2021

Department of Microbiology & Immunology
National Cheng Kung University
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

GeneTex

Product Name:

ATG5 antibody

Catalog Number:

GTX113309

Clone Number:

N/A

This antibody was used to detect ATG5 protein expression after conditioned medium (CM) treatment in MEF cells by western blot analysis.

Experimental Design and Results Summary

Applications

Western Blot

Sample

MEF cell extracts

Primary Incubation

1:5000 o/n at 4 degree

Blocking Agent

5% skimmed milk in TBST

Secondary Incubation

1:10000 for 2 hr at RT

Tertiary Incubation

N/A

Detection

HRP substrate

Results Summary

Clear signal in correct molecular weight.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

High sensitivity

The Bad

No

The Bottom Line

We will continue to use this antibody.

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