BD
PE Mouse Anti-Human CD127
557938
HIL-7R-M21
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Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody secreting cells (ASC) and B cells in health and disease. Here, we aimed at staining CD127 in isolated PBMCs from peripheral blood of a healthy donor (HD).
Flow Cytometry
Isolated PBMCs from HD peripheral blood
surface staining with CD127 PE (1/10), 20 min at 4C
Fc block
N/A
Flow cytometry
We could stain CD127 that is expressed in T cells in the periphery.
Very bright
NA
Good option