Very Good Staining for MHCII

May 03, 2021

Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Brilliant Violet 711™ anti-mouse I-A/I-E Antibody

Catalog Number:

107643

Clone Number:

M5/114.15.2

We are interested in characterizing the immune populations in secondary lymphoid organs and tumors under different knockout condition.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse MC38 Tumor

Primary Incubation

4deg 30min 1:500

Blocking Agent

Fc block

Secondary Incubation

None

Tertiary Incubation

None

Detection

Cytek Aurora

Results Summary

Very distinct population that are MHCII+ and CD80+ in the tumor microenvironment.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Very consistent quality.

The Bad

None

The Bottom Line

Very good clone for staining MHCII.

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