Highly Recommended Assay Kit for COVID-19 Spike ACE2 Binding

Stanford University
Pediatrics
Lab Director / Manager

Overall

Quality of Results

Ease-of-Optimization

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Company:

RayBiotech

Product Name:

COVID-19 Spike-ACE2 Binding Assay Kit; 1 x 96-Well Strip Microplate Kit

Catalog Number:

CoV-ACE2S2-1

I used supernatant from my in-vitro cultures and the assay worked well.It doesn't matter if you run 1 slide or 4, it will take the same time so I did run all the slides at the time, and in that way you can run only one set of standards which allow you to run some extra samples.Things I didnt like: my slides came with a green handwriting label in each slide, that interfered with the final result of the test, since it creates a red shadow that you can see in the pictures.This is something not specifically related with the kit, but every package I receive for RayBiotech comes with a "storage temperature:-20C" sticker on the sipping box, but part of the kit should be at 4C. So be careful when you receive your package. The package came when I was not in the lab and my coworker didn't check, so my whole kit end up at -20 overnight. I mentioned that to the company and they said the kit will be ok, but it was not. I had to order a third one.

Experimental Design and Results Summary

Application

Medical

Starting Material

N/A

Tips

Label each slide with a tiny dot in a corner to recognize the up/down.

Results Summary

It worked. But is kind of annoying and time consuming to place correctly each dot in the correct position one by one in order to be able to run the analysis. All my slides had one raw in a weird position, so I had to place manually the area for analysis. I dont know if this is something you should expect or my kit was defective.

DOI or PMID #

N/A

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Summary

The Good

It is clear when works and when doesnt work.

The Bad

Time consuming, you should be extra careful to not swap your sample from the slide when reading it in the equpment.

The Bottom Line

Great signal, fast shipping, reasonable price, time consuming, recommended.

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