Biolegend
PE/Dazzle™ 594 anti-human CD4 Antibody
344640
SK3
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Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody secreting cells (ASC) and B cells in health and disease. Here, we aimed at staining CD4 T cell subsets in isolated PBMCs from peripheral blood of a healthy donor (HD).
Flow Cytometry
Isolated PBMCs from HD peripheral blood
surface staining with CD4 PEDazzle 594 (1/1000), 20 min at 4C
Fc block
N/A
Flow cytometer.
We could detect CD4 T cells (CD3+CD4+).
The antibody works well.
Highly recommended.