Unspecific Staining in Human Pancreas Tissue Section

May 16, 2021

BlueskyReddit
Type 1 Diabetes Center
La Jolla Institute for Immunology
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Biolegend

Product Name:

Alexa Fluor® 647 anti-Tyrosine Hydroxylase Antibody

Catalog Number:

818008

Clone Number:

2/40/15

We used the anti-Tyrosine Hydroxylase antibody to stain for the sympathetic neurons in human pancreatic tissues. These are FFPE tissue sections, we used a standard protocol with citrate buffer pH 6 for antigen retrieval and blocked with 10% normal goat serum.The end result is that no Neurons were detected and the whole tissue lit up, so unspecific staining at least in our case.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Human pancreatic tissues

Primary Incubation

1:100, overnight at 4 degrees

Blocking Agent

10% normal goat serum

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Axioscanner

Results Summary

We used the anti-Tyrosine Hydroxylase antibody to stain for the sympathetic neurons in human pancreatic tissues. These are FFPE tissue sections, we used a standard protocol with citrate buffer pH 6 for antigen retrieval and blocked with 10% normal goat serum.The end result is that no Neurons were detected and the whole tissue lit up, so unspecific staining at least in our case.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

N/A

The Bad

Unspecific staining

The Bottom Line

-Does not work on human pancreas tissue-Unspecific staining

Share your experience with other scientists. Write a Review! »

Join the discussion