R&D Systems
p38 alpha Antibody
AF8691
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Browse Similar Products: p38 Western Blot Antibodies
We were looking for an antibody to test the time-dependent role of a growth factor on p38 protein level in mammalian smooth cells. p38 is a well-known MAPK which regulates many genes. Our antibody search was ended with the R&D Systems, and it was inspiring to see the antibody specificity which was indicated in the flyer. Thus, to complete our goal, the log phase of growth, arrested cells were treated with specific growth factor for a different period. From these cells the protein extract was prepared and resolved in 10 % SDS-PAGE. The resolved protein was western transferred on the nitrocellulose membrane and probed for p38 using R&D Systems Ab.
Western Blot
Mammalian whole-cell protein lysate
Dilution (1:1000) in 5% skimmed milk prepared in 1x TBST, overnight incubation at 4 degree Celsius
5% skimmed milk made in 1x TBST
Goat anti-mouse IgG HRP Ab, dilution (1:4000) in 5% skimmed milk made in 1x TBST, 1hr at RT
None
Enhanced chemiluminescent detection in X-Ray film
R&D Systems antibody has detected only a single band of p38 with high specificity in the mammalian protein sample.
A specific band of p38 were observed
State of the art R&D Systems antibody for Immunodetection of p38 in mammalian cells