Bacterial OMV Isolation Kit

University of Texas Medical Branch at Galveston
OBGYN
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Systems Biosciences

Product Name:

ExoBacteria™M OMV Isolation Kit

Catalog Number:

Cat # EXOBAC100A-1

I needed this for isolation of OMVs from bacterial cells. Works great, and the steps are very easy to do.

Experimental Design and Results Summary

Application

OMV isolation

Starting Material

N/A

Tips

You just need time to prepare your bacterial culture. After that, 30 mL of culture media will give you around 1.5 mL of OMVs.

Results Summary

We have good sized OMVs from bacteria, and these have consistent range of concentrations and diameters. These are also immunogenic based on functional tests we did.

DOI or PMID #

N/A

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Summary

The Good

Fast and reliable isolation procedure. May adjust final elution buffer concentration depending on target concentration.

The Bad

Experiments are limited to 30 mL of bacteria only.

The Bottom Line

Expensive, but really works!

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