Thermo Fischer Scientific
FOXP3 Monoclonal Antibody (FJK-16s), FITC, eBioscience™
11-5773-82
FJK-16s
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In different healthy and pathological contexts, CD4 T cells differentiate into a range of specialized effector and regulatory subsets. In mouse models of aging and autoimmune diseases we study the plasticity and functions of CD4 T regulatory cells (Tregs). We identified Tregs with an anti-FOXP£ FITC antibody in old C57Bl/6J mice.
Flow Cytometry
Splenocytes of wild-type 24 months-old C57Bl/6J mouse, incubated in complete culture medium at 37 °C with (Restimulated) or without (unstimulated) PMA/ionomycin for 4h and brefeldin A for the 3 last hours.
Washed twice in PBS and incubated with L/D fixable aqua dye 20 min at 4°C, then washed twice in PBS.
2.4G2
Surface staining with anti-CD3 BUV805, anti-CD4 BUV495, etc., in Cell Staining Buffer (Biolegend) incubated 30 min at 4°C.
Cells were fixed and permeabilized with commercial kit (TrueNuclear Biolegend) for 45 min at 4°C with fixative, 15 min at 4°C with permeabilization buffer, and stained for cytokines and anti-FOXP3 FITC 1/25 in permeabilization buffer.
Blue laser on Symphony cytometer
FOXP3+ cells were detected. The staining sepated well the positive population, even on activated cells
N/A
Easy to use and bright staining.
Expensive
Good antibody but expensive because it requires high concentration.