Low CD64-FITC Quality

Singh Center for Nanotechnology

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

CD64 (FcgammaRI) Antibody

Catalog Number:

139315

Clone Number:

N/A

I used this antibody for flow cytometry, and the signal quality was really low. 10^6 cells were stained with CD64 at 1:100 dilution on ice for 30 min. Stained cells were analyzed immediately by LSR II Flow Cytometer.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Macrophages

Primary Incubation

1:100 30 min 4 °C

Blocking Agent

5% FBS; RT, 30 min

Secondary Incubation

None

Tertiary Incubation

None

Detection

LSR II Flow Cytometer

Results Summary

This antibody gives a really weak signal. I tried this antibody three times and I have never got great results.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Signal detected.

The Bad

Low signal.

The Bottom Line

Not recommended.

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