BD Biosciences
BD OptiBuild™ BUV496 Rat Anti-Mouse CD4
741051
RM4-4
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In different healthy and pathological contexts, CD4 T cells differentiate into a range of specialized interleukin-secreting subsets. In mouse models of aging and autoimmune diseases we study the plasticity and effector functions of CD4 T helper cells. We used anti-CD4 to analyze the CD4 T cell population in old C57Bl/6J mice.
Flow Cytometry
Splenocytes of wild-type 24 months-old C57Bl/6J mouse, incubated in complete culture medium at 37 °C with (Restimulated) or without (unstimulated) PMA/ionomycin for 4h and brefeldin A for the 3 last hours.
Washed twice in PBS and incubated with L/D fixable aqua dye 20 min at 4°C, then washed twice in PBS.
2.4G2
Surface staining with anti-CD3 BUV805, anti-CD8 FITC, anti-CD4 BUV495 1/150, etc., in Cell Staining Buffer (Biolegend) incubated 30 min at 4°C.
Cells were fixed and permeabilized with a commercial kit to counterstain for cytokines.
UV laser on Symphony cytometer
We could identify and CD4 T cells, even after stimulation. The antibody works well and does not give compensation problems.
N/A
Bright even after stimulation of the cells.
It is expensive.
Can be used advantageously to expend panels with the use of UV laser. Good option to identify cells even after stimulation.