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Brilliant Violet 650™ anti-mouse Ly-6C Antibody
128049
HK1.4
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Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of B cells and antibody-secreting cells (ASC) in health and disease, in this experiment we are looking for markers of PC.
Flow Cytometry
Mouse splenocytes
Surface staining with Ly-6C BV650 (1/800), 20 min at 4C
Fc block (2.4G2 clone)
N/A
Flow cytometer
In this work, we were looking for surface markers that are differentially expressed by B cells (CD19+) and plasma cells (PC). But in order to test the antibody and knowing that some CD4 T cells express Ly6C, we use CD4+ T cells as positive control.
Accessible and high quality.
Antibody has great signal. I can recommend it.