Mini Magnetic Columns for Negative Selection Isolation

July 29, 2022

BlueskyReddit
University of Texas Medical Branch at Galveston
OBGYN
Graduate Student

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Company:

Miltenyi Biotec

Product Name:

MS Columns

Catalog Number:

130-042-201

We are isolating trophoblasts from placental tissues, and the prefinal step involves the separation of trophoblasts from stromal cells. The columns allow for negative selection using magnetic beads conjugated to stromal antibodies, but not all of them are filtered through.

Experimental Design and Results Summary

Application

Cell isolation

Starting Material

N/A

Tips

Prevent bubbles from entering the column. Clogs may occur if suspension has contaminants that may block the path (e.g., blood clots, debris aggregates etc). May push down with plunger to increase filtration speed.

Results Summary

Despite negative selection, we still get contaminating stromal cells after the initial run through. This is not significant, although other factors such as the antibody used may affect isolation as well.

DOI or PMID #

N/A

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Summary

The Good

Low volume applicable. Small footprint. Quick and easy.

The Bad

May not totally isolate the desired population.

The Bottom Line

The benefits of using magnetic selection as a cell isolation technique is still greater than its drawbacks. The column is a much faster and more convenient alternative to other cell isolation techniques (e.g., FACS sorting).

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