BD Biosciences
PE-Cy™7 Rat Anti-Mouse CD127
560733
SB/199 (RUO)
Info:Supplier Page
Browse Similar Products: CD127 Antibodies
In different healthy and pathological contexts, CD4 T cells differentiate into a range of specialized interleukin-secreting subsets. In mouse models of aging and autoimmune diseases we study the plasticity and effector functions of CD4 T helper cells. We analyzed naïve and memory T cells with an anti-CD127 PE Cy7 antibody in old C57Bl/6J mice.
Flow Cytometry
Splenocytes of wild-type 24 months-old C57Bl/6J mouse, incubated in complete culture medium at 37 °C with (Restimulated) or without (unstimulated) PMA/ionomycin for 4h and brefeldin A for the 3 last hours.
Washed twice in PBS and incubated with L/D fixable aqua dye 20 min at 4°C, then washed twice in PBS.
2.4G2
Surface staining with anti-CD3 BUV805, anti-CD4 BUV495, anti-CD127 PE Cy7 1/800, etc., in Cell Staining Buffer (Biolegend) incubated 30 min at 4°C.
Cells were fixed and permeabilized with a commercial kit to counterstain for cytokines.
Yellow-green laser on Symphony cytometer
CD127+ and CD127- memmory CD44+ T cells were distinguished. CD127 on naïve CD44- cells is downregulated upon stimulation.
N/A
Excellent value for money.
Need to be carefully with spill over from other far-red antibodies.
Good option, to be recommended.