Excellent Anti-Mouse CD3 Antibody (FITCH)

October 31, 2022

BlueskyReddit
LP2M
University of Cote d'Azur (UCA)
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

FITC anti-mouse CD3 Antibody

Catalog Number:

100203

Clone Number:

17A2

Info:Supplier Page View Product Specs

Browse Similar Products: CD3 Antibodies

Our work requires is focused on the mouse lymphocytes T population. This antibody provides an excellent marker for FACS staining of T cells

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Primary mouse cells (spleenocytes/isolated cells)

Primary Incubation

1:100 per 200-500k cells in 100ul

Blocking Agent

PBS + FCS 2%

Secondary Incubation

None

Tertiary Incubation

None

Detection

FACS Aria II

Results Summary

Excellent staining for total T cells in both total spleenocytes or isolated cells. No aspecific staining has been observed so far.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Excellent staining.

The Bad

None

The Bottom Line

This antibody has excellent specificity and the cost is relatively low compared with other suppliers for the same clone.

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