CD182 Antibody with Very Strong Signal

November 07, 2022

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE anti-mouse CD182 (CXCR2) Antibody

Catalog Number:

149304

Clone Number:

SA044G4

Info:Supplier Page View Product Specs

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Used to study the plasma membrane localization and receptor trafficking of CXCR2. PE signal is very strong.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse bone marrow cellls

Primary Incubation

1:60 30 minutes on ice, 50uL, 5e6 cells.

Blocking Agent

FC block, 15 minutes on ice

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

YETI analyser

Results Summary

Upon neutrophil priming with GM-CSF and TNF-alpha, CXCR2 is shed from the cell surface. Cells were stained with antibody cocktail to identify live neutrophils and CXCR2.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Very reliable.

The Bad

Use at high concentration.

The Bottom Line

Essential antibody for the study of CXCR2 in mouse neutrophils.

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