Caspase-Glo® 3/7 Assay for Apoptosis Detection

November 04, 2022

Robert Bosch Gesellschaft für medizinische Forschung mbH
IKP/RBCT
Graduate Student

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Company:

Promega

Product Name:

Caspase-Glo® 3/7 Assay Systems

Catalog Number:

G8090, G8091, G8093 and G8092

We regularly detect apoptosis in several human cancer cell lines using the Caspase-Glo® 3/7 Assay from Promega. The protocol is easy to handle and the measured luminescence is robust, reliable and very sensitive (even with small amounts of cell suspension).

Experimental Design and Results Summary

Application

Apoptosis detection

Starting Material

Human cells in a white-bottom 96-well plate, conventional luminometer.

Tips

We obtained good results even with 10 % of the stated glo substrate volume. Just make sure the ratio of cell medium and substrate remains 1:1 (e.g. 10 µl cells + 10 µl substrate). Can easily be combined with generation of Western Blot samples.

Results Summary

We obtained robust cell death data for several treatments/conditions in human cancer cell lines e.g. in a recent project about the synergism of the BH-3 mimetic ABT-199 and proteasome inhibitor Bortezomib.

DOI or PMID #

10.1158/1538-7445.AM2022-3706

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Summary

The Good

Fast protocol. Robust signal. Very sensitive.

The Bad

Pricy. In some cases data normalization is needed in addition (e.g. transfections).

The Bottom Line

The Caspase-Glo® 3/7 Assay Systems are a quick and easy way to obtain robust cell death data even if you have few cells to spare.

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