Great PI Staining Buffer

Houston Methodist Research Institute
Nanomedicine
Postdoctoral Researcher

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Company:

BD Pharmingen

Product Name:

PI/RNase staining Buffer

Catalog Number:

550825

The goal of our research is to check the drug effect on the MDA-MB-231 cell cycle. We used this PI staining buffer to stain fixed cell and analyzed by flow cytometry. The result is great. We can clearly tell cell cycle stages.

Experimental Design and Results Summary

Application

Cell cycle analysis

Starting Material

N/A

Tips

Use cold 70% ethanol drop by drop into the loosed cell pellet while vortex.

Results Summary

Using this buffer to analyze the cell cycle by flow cytometry, we find the MDA-MB-231 cell population was arrested in the G2 phase after drug treatment.

DOI or PMID #

N/A

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Summary

The Good

Reliable.

The Bad

N/A

The Bottom Line

Great PI staining buffer for cell cycle analysis. Highly recommend!

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