pBluescript® II SK(-) Phagemid Kit
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Agilent Technologies
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Product
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pBluescript® II SK(-) Phagemid Kit
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Company
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Agilent Technologies
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Price
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More Information
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View Company Product Page
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Accession Number
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Request Info
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Applications
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• High-resolution restriction mapping
• Creation of exo/mung nested deletions
• Single-stranded rescue
• Double- and single-stranded sequencing
• In vitro RNA transcription
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Copy Number
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Request Info
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Host
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Bacteria
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Inducer
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Request Info
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MCS
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Cloning capacity of 12 kb
12 unique cloning sites in amino-terminal region of lacZ gene
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Origin of Replication
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pUC ori
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Promoter
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Expression of fusion proteins from lac promoter
In vitro RNA transcription with T3 or T7 RNA polymerase
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Quantity
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1 kit
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Recombination Site
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Request Info
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Reporter
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Request Info
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Resistance
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Ampicillin-resistance in E. coli
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Selectable Marker
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Blue/white color screening
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Type
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3.0 kb
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Vector Name
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pBluescript® II Vector
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Catalog Number
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212206
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Description
High-resolution restriction mapping. Creation of exo/mung nested deletions. Single-stranded rescue. Double- and single-stranded sequencing. In vitro RNA transcription
'f1 origin in (-) orientation, Sac-->Kpn, polylinker orientation, Contains: 20 µg pBluescript II SK(-) phagemid vector, Host Strain: XL1-Blue MRF'
MCS: KpnI, ApaI, Eco0109I, XhoI, SalI, Bsp106I, HindIII, EcoRV, EcoRI, PstI, SmaI, BamHI, SpeI, XbaI, NotI, EagI, SacII, BstXI, SacI
Selectable Marker: ampicillin
Specifications/Features
Kit Contents:
f1 origin in (-) orientation, Sac-->Kpn, polylinker orientation, Contains: 20 µg pBluescript II SK(-) phagemid vector, Host Strain: XL1-Blue MRF'
Vector Size:
3.0 kb