Polymerase chain reaction (PCR) amplifies DNA by copying the nucleic acid strands numerous times in vitro. PCR depends on the availability of thermostable enzymes in order to cycle between the temperatures needed to melt and anneal the DNA strands. PCR is used for numerous applications including DNA sequencing, cloning, library generations, mutagenesis, expression profiling, and more. PCR requires instrumentation, reagents, and consumables like thermal cyclers, PCR kits, buffers, enzymes, primers, bases, and so forth. In selecting products, it is helpful to work with a vendor that will provide optimized protocols, documentation, and troubleshooting assistance, since the systems can be complex with many sources of error.
Thermocycler manufacturers offer guidance in this Q&A.
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dPCR, an advanced method for NA quantification, is increasingly used
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Choose the CFX Opus real-time PCR system for the reliability you depend on from Bio-Rad with improved connectivity and performance for your modern lab. The CFX Opus system is the next evolution in qPCR from Bio-Rad. With improved thermal performance ...
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The QX Continuum Droplet Digital PCR (ddPCR) System combines the hallmark performance of ddPCR technology with the user-friendly experience of qPCR. It provides cost-effective absolute quantification of up to four targets from a single sample well, ...
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We study how certain mutations affect viral replication. We construct our mutant cDNA infectious clones by PCR and this DNA polymerase mx is extremely convenient to this end. In this case, my reaction consisted of mixing my template DNA (a Dengue ...
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I recommend this because it saves a lot of time, and works well. I didn't recycle the gel product.
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