Protein purification is a staple of most pharmaceutical, biotech, and basic research labs. Multifarious protein purification methods and systems, from immunoprecipitation to chromatography are available, all of which require meticulous sample handling and solid research tools. Fundamental considerations for devising a protein purification plan include finding the right method, reagents, and instruments. Researchers require distinct tools which depend on their end goal, such as purifying proteins for antibody production or for therapeutics. Homogenizers, buffers, and protease inhibitors are needed to extract protein from its source. Products for extracting organelle proteins are also distributed to avoid cellular total protein contamination. To increase working quantity extracted proteins can be concentrated with products such as dialysis membranes. HPLC or GC chromatography are fast, high throughput methods of protein purification. Low-scale systems, like affinity kits are available for smaller sample volume. Researchers can acquire assistance by investigating listed protein purification service and user guide providers.
The biotin-streptavidin bond is the strongest noncovalent biological interaction...
Immunoprecipitation (IP) is a frequently used method for purifying specific ...
Wednesday, April 27, 2011
Sometimes there is no better substitute for getting to your protein(s) of interest than simply ...
There are at least three reasons to prepare samples carefully prior to high-pressure liquid ...
Thursday, September 20, 2012
Rigaku's Minstrel DT UV is an ultraviolet and visible crystal imaging and protein crystal monitoring system. Providing the highest optical resolution for combined visible and UV spectrum imaging, the Minstrel DT UV allows users to detect smaller ...
Tuesday, January 31, 2012
Quantitation of proteotypic peptides of plasma by SRM-MS allows specific, internally-standardized measurement of protein biomarkers and can achieve sub-nanogram/ml detection levels when specific anti-peptide antibodies are used to enrich target ...
For batch processing of up to 50 microplates, the Infinite M1000 PRO microplate reader can be equipped with an integrated Stacker module.
Monday, February 27, 2012
GE Healthcare’s HiTrap™ TALON crude, His SpinTrap™ TALON microspin, His MultiTrap™ TALON and His GraviTrap™ TALON pre-packed with TALON Superflow™ ...
GE Healthcare’s rProtein A GraviTrap™, Protein G GraviTrap and rProtein A/Protein G GraviTrap, gravity-flow columns enable single-step, efficient ...
Bio-Rad Laboratories has published new methods for using the Profinity eXactTM fusion-tag system to express recombinant proteins in eukaryotic and ...