Chromatography is the broad term for laboratory techniques designed for the separation or analysis of complex mixtures. The mixture is dissolved in a fluid called the mobile phase, which carries it through a structure holding another material called the stationary phase. Different rates of migration cause the various constituents of the mixture to travel at different speeds, causing them to separate. Chromatography is used in many research and clinical labs in applications such as isolating new compounds and analyzing differences between compounds. Chromatography equipment includes all the components needed for separation: columns, frits, flow cells, pumps, detector, collectors and software to complete systems used for High Performance Liquid Chromatography (HPLC), Gas chromatography (GC) and Liquid Chromatography and Mass Spectrophotometers (LC-MS). It is important to consider the nature of the sample when choosing chromatography equipment. The current platform being used and it’s compatibility with other components, and the space available in the laboratory are other factors to consider when selecting proper equipment. Chromatography is often the first step in an assay; downstream applications depend on the purity and configuration of its product.
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This video illustrates the principles of Ion Exchange Chromatography. Ion Exchange Chromatography is used to purify biomolecules and separate the molecules according to differences in their net surface charge.
This video illustrates the principles of Affinity Chromatography. Affinity Chromatography depends upon the reversible adsorption of biomolecules through biospecific interactions on the ligand. GE Healthcare Life Sciences provides a wide range of ...
Ed Thompson, CEO at Pharmaceutical Manufacturing Research Services, Inc. (PMRS), describes how using Agilent OpenLAB Enterprise Content Manager (ECM) eliminates the bottlenecks and human error associated with a paper-based supply of documentation to ...
LECO Corporation has introduced Internet-based training classes for its Separation Science instrumentation.
Friday, November 04, 2011
TriMarkPublications.com cites in its newly published "Mass Spectroscopy for Clinical Laboratory Analysis" report that the mass spectroscopy market is ...
Tuesday, February 08, 2011
Agilent Automation Solutions has announced a new white paper entitled 'An introduction to High-throughput Microchromatography' which introduces a ...