RNA interference (RNAi) is the process by which the translation of a protein is prevented by selective degradation of its encoded mRNA. In nature, this mechanism likely evolved for cells to eliminate unwanted foreign genes as a defense against viruses. In research, this technique is used for loss-of-function studies. RNAi has had significant impact on the ease, speed, and specificity with which the loss of gene function analysis can be done in mammalian cells and animal models. RNAi technology has the ability to validate target genes and functionally assess relevant disease genes. Thus leading to the development of effective therapeutics. The discovery of RNA interference by Craig Mello and Andrew Fire earned them the 2006 Nobel Prize for Physiology or Medicine. Highlighting the importance of this technology on the future of disease research and drug development.
The most widely employed method of achieving post-transcriptional gene silencing...
The TaqMan® MicroRNA (miRNA) Assays from Applied Biosystems were developed to ...
Thursday, October 17, 2013
RNAi is simple in concept yet nuanced in implementation. Key to success is optimization. The right conditions ...
Whether long, small or micro, noncoding RNAs are not translated into proteins, yet fulfill important ...
Stephanie Culbertson discusses success with Lentiviral based RNAi. Watch Video
Friday, February 15, 2008
Stephanie Fulmer discusses the addition of Triton X-102 to wash buffers for the miRNA and gene expression microarray applications. Watch Video
Thursday, February 14, 2008
Mark Landers discusses miRNA Expression in Cancer. Watch Video